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(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
(2R,3S)-3-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH + H+
(2R,3S)-3-isopropylmalate + NAD+
2-ketoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxovalerate + CO2 + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
?
(2R,3S)-3-isopropylmalate + NAD+
D-3-isopropyl-2-oxosuccinate + NADH + H+
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
2-oxoisohexanoate + thionicotinamideNADH + CO2
(S)-malate + NAD+
pyruvate + NADH + CO2
-
Substrates: 12% of the activity with 3-isopropylmalate
Products: -
?
3-(2'-methylthio)ethylmalate + NAD+
? + NADH + H+
Substrates: -
Products: -
?
3-ethylmalate + NAD+
3-ethylpyruvate + NADH + CO2
3-isoamylmalate + NAD+
3-isoamylpyruvate + NADH + CO2
-
Substrates: -
Products: -
?
3-isopropylmalate + NAD+
2-ketoisocaproate + CO2 + NADH + H+
3-isopropylmalate + NAD+
2-ketoisocaproate + NADH + CO2
3-isopropylmalate + NAD+
?
-
Substrates: -
Products: -
?
3-methylmalate + NAD+
3-methylpyruvate + NADH + CO2
-
Substrates: -
Products: -
?
3-tert-butylmalate + NAD+
3-tert-butylpyruvate + NADH + CO2
-
Substrates: -
Products: -
?
beta-methylmalate + NAD+
2-oxobutyrate + NADH + CO2
-
Substrates: enzyme catalyzes the final step in the pyruvate pathway to 2-oxobutyrate
Products: -
?
D-3-ethylmalate + NAD+
D-3-ethyl-2-oxosuccinate + NADH + H+
D-3-isobutylmalate + NAD+
D-3-isobutyl-2-oxosuccinate + NADH + H+
D-3-isopentylmalate + NAD+
D-3-isopentyl-2-oxosuccinate + NADH + H+
D-3-methylmalate + NAD+
D-3-methyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
D-3-t-butylmalate + NAD+
D-3-t-butyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
D-malate + NAD+
pyruvate + NADH + CO2
DL-3-isopropylmalate + NAD+
DL-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
erythro-Lg-beta-isopropylmalate + NAD+
?
-
Substrates: -
Products: -
r
ethylmalate + NAD+
2-oxopentanoate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
homoisocitrate + NAD+
? + NADH + H+
isopropylmalate + NAD+
?
-
Substrates: -
Products: -
?
malate + NAD+
3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
malate + NAD+
pyruvate + NADH + H+ + CO2
malate + NADP+
pyruvate + NADPH + CO2
-
Substrates: -
Products: -
?
propylmalate + NAD+
?
-
Substrates: -
Products: -
r
tartrate + NAD+
?
-
Substrates: -
Products: -
?
tartrate + NADP+
?
-
Substrates: -
Products: -
?
tert-butylmalate + NAD+
2-hydroxy-4,4-dimethylpentanoate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
threo-D-3-isopropylmalate + NAD+
?
threo-DL-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH
additional information
?
-
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
r
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
r
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
r
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: mutant enzymes with increased activity
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
r
(2R,3S)-3-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
r
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: a large movement of domain 1 is induced by substrate binding, which results in the formation of the hydrophobic pocket for the gamma-isopropyl moiety of isopropylmalate
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: key enzyme in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
Bacillus sp. (in: Bacteria) no 38-2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: erythro-Lg-beta-isopropylmalate
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: the enzyme catalyzes the third pathway-specific reaction in the biosynthesis of leucine
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: penultimate step in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: enzyme is involved in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
639130, 639131, 639136, 639138, 639139, 639140, 639146, 639152, 639153, 639155, 639156, 639157, 639158, 639159, 639160, 639164, 639165, 639167, 724976 Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: decarboxylation appears to take place with retention of configuration
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: enzyme of leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: key enzyme in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: the enzyme belongs to the leucine biosynthetic pathway
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: the enzyme belongs to the leucine biosynthetic pathway
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
?
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
?
Substrates: the enzyme is enzyme involved in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
D-3-isopropyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
D-3-isopropyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
2-oxoisohexanoate + thionicotinamideNADH + CO2
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + thionicotinamide NAD+
2-oxoisohexanoate + thionicotinamideNADH + CO2
-
Substrates: -
Products: -
?
3-ethylmalate + NAD+
3-ethylpyruvate + NADH + CO2
-
Substrates: -
Products: -
?
3-ethylmalate + NAD+
3-ethylpyruvate + NADH + CO2
-
Substrates: DL-erythro-beta-ethylmalate
Products: -
?
3-isopropylmalate + NAD+
2-ketoisocaproate + CO2 + NADH + H+
-
Substrates: -
Products: -
?
3-isopropylmalate + NAD+
2-ketoisocaproate + CO2 + NADH + H+
-
Substrates: the enzyme catalyzes a step in leucine biosynthesis
Products: -
?
3-isopropylmalate + NAD+
2-ketoisocaproate + NADH + CO2
-
Substrates: -
Products: -
?
3-isopropylmalate + NAD+
2-ketoisocaproate + NADH + CO2
Substrates: -
Products: -
?
D-3-ethylmalate + NAD+
D-3-ethyl-2-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
D-3-ethylmalate + NAD+
D-3-ethyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
D-3-ethylmalate + NAD+
D-3-ethyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
D-3-isobutylmalate + NAD+
D-3-isobutyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
D-3-isobutylmalate + NAD+
D-3-isobutyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
D-3-isopentylmalate + NAD+
D-3-isopentyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
D-3-isopentylmalate + NAD+
D-3-isopentyl-2-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
D-malate + NAD+
pyruvate + NADH + CO2
-
Substrates: 19% of the activity with 3-isopropylmalate
Products: -
?
D-malate + NAD+
pyruvate + NADH + CO2
-
Substrates: -
Products: -
?
D-malate + NAD+
pyruvate + NADH + CO2
-
Substrates: -
Products: -
?
D-malate + NAD+
pyruvate + NADH + CO2
-
Substrates: -
Products: -
?
homoisocitrate + NAD+
? + NADH + H+
Substrates: -
Products: -
?
homoisocitrate + NAD+
? + NADH + H+
Substrates: -
Products: -
?
malate + NAD+
pyruvate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
malate + NAD+
pyruvate + NADH + H+ + CO2
-
Substrates: -
Products: -
?
threo-D-3-isopropylmalate + NAD+
?
Substrates: domain 1 binds the coenzyme NAD+, while the substrate 3-isopropylmalate binds mainly to domain 2
Products: -
?
threo-D-3-isopropylmalate + NAD+
?
Substrates: domain 1 binds the coenzyme NAD+, while the substrate 3-isopropylmalate binds mainly to domain 2
Products: -
?
threo-D-3-isopropylmalate + NAD+
?
-
Substrates: domain 1 binds the coenzyme NAD+, while the substrate 3-isopropylmalate binds mainly to domain 2
Products: -
?
threo-D-3-isopropylmalate + NAD+
?
-
Substrates: domain 1 binds the coenzyme NAD+, while the substrate 3-isopropylmalate binds mainly to domain 2
Products: -
?
threo-DL-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH
Substrates: -
Products: -
?
threo-DL-isopropylmalate + NAD+
2-isopropyl-3-oxosuccinate + NADH
-
Substrates: -
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes a step in leucine biosynthesis
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, e.g. L103, T105, A337, and T341, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
-
Substrates: enzyme does not catalyze the oxidation of L-malate, L-tartrate, D-tartrate, DL-isocitrate or DL-lactate
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
-
Substrates: no activity with threo-Ds-isocitrate
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
-
Substrates: no activity with threo-Ds-isocitrate
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: the enzyme does not show detectable activity for isocitrate using NAD+ as a coenzyme, and is inactive with NADP+
Products: -
?
additional information
?
-
-
Substrates: the enzyme does not show detectable activity for isocitrate using NAD+ as a coenzyme, and is inactive with NADP+
Products: -
?
additional information
?
-
Substrates: the enzyme does not show detectable activity for isocitrate using NAD+ as a coenzyme, and is inactive with NADP+
Products: -
?
additional information
?
-
Substrates: no substrate: isocitrate
Products: -
?
additional information
?
-
Substrates: no substrate: isocitrate
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
-
Substrates: reaction scheme based on incorporation of the rate constant of a fast protein conformational change such as domain closure derived from the separately recorded time-dependent formation of the fluorescence resonance energy transfer signal. The rate-limiting step seems to be another slower conformational change that allows product release
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
additional information
?
-
Substrates: substrate specificity-determining residues, overview
Products: -
?
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(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxovalerate + CO2 + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
?
Substrates: the enzyme is enzyme involved in leucine biosynthesis
Products: -
?
3-isopropylmalate + NAD+
2-ketoisocaproate + CO2 + NADH + H+
-
Substrates: the enzyme catalyzes a step in leucine biosynthesis
Products: -
?
additional information
?
-
-
Substrates: the enzyme catalyzes a step in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
r
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
(2S)-2-isopropyl-3-oxosuccinate + NADH + H+
Substrates: -
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: key enzyme in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: the enzyme catalyzes the third pathway-specific reaction in the biosynthesis of leucine
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: penultimate step in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: enzyme is involved in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: enzyme of leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
2-oxoisocaproate + NADH + H+ + CO2
-
Substrates: key enzyme in leucine biosynthesis
Products: -
?
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: the enzyme belongs to the leucine biosynthetic pathway
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: the enzyme belongs to the leucine biosynthetic pathway
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
-
Substrates: -
Products: -
ir
(2R,3S)-3-isopropylmalate + NAD+
4-methyl-2-oxopentanoate + CO2 + NADH + H+
Substrates: -
Products: -
?
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
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0.0035 - 3.73
(2R,3S)-3-isopropylmalate
0.045 - 0.502
3-(2'-methylthio)ethylmalate
0.0039 - 0.0254
3-isopropylmalate
0.074
3-tert-butylmalate
-
-
0.0011
D-3-ethylmalate
pH 8.0
0.0015
D-3-isobutylmalate
pH 8.0
0.0058
D-3-isopentylmalate
pH 8.0
0.0012
D-3-isopropylmalate
pH 8.0
0.0037
D-3-methylmalate
pH 8.0
0.0023
D-3-t-butylmalate
pH 8.0
0.002 - 0.0133
DL-3-isopropylmalate
0.019
dl-erythro-beta-ethylmalate
-
pH 9.0, 22°C
0.0092
erythro-Lg-beta-isopropylmalate
-
pH 9.0, 22°C
0.09
ethylmalate
-
pH 7.6
5.9
homoisocitrate
at pH 8.0 and 60°C
0.000017 - 0.316
isopropylmalate
0.105
propylmalate
-
pH 7.6
0.214
tert-butylmalate
-
pH 7.6
0.051
thionicotinamide-NAD+
-
pH 9.0, 22°C
0.0061 - 0.0161
threo-D,L-isopropylmalate
0.08
threo-Ds-3-Isopropylmalate
-
60°C
additional information
additional information
-
0.0035
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.5 and 25°C
0.0047
(2R,3S)-3-isopropylmalate
-
acIPMDH-ML, pH 8.0, 40°C
0.005
(2R,3S)-3-isopropylmalate
at pH 8.0 and 60°C
0.0053
(2R,3S)-3-isopropylmalate
-
ancIPMDH-IQ, pH 8.0, 40°C
0.0063
(2R,3S)-3-isopropylmalate
mutant enzyme Y181H, at pH 7.5 and 25°C
0.0074
(2R,3S)-3-isopropylmalate
mutant enzyme Y181F, at pH 7.5 and 25°C
0.0083
(2R,3S)-3-isopropylmalate
mutant enzyme Y181A, at pH 7.5 and 25°C
0.0087
(2R,3S)-3-isopropylmalate
-
pressure 100 MPa, 20°C, pH not specified in the publication
0.0092
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.01
(2R,3S)-3-isopropylmalate
-
acIPMDH-ML, pH 8.0, 70°C
0.01
(2R,3S)-3-isopropylmalate
-
ancIPMDH-IQ, pH 8.0, 70°C
0.0109
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.011
(2R,3S)-3-isopropylmalate
-
pH 7.5, 37°C
0.011
(2R,3S)-3-isopropylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
0.0114
(2R,3S)-3-isopropylmalate
mutant enzyme V235A, at pH 7.5 and 25°C
0.0122
(2R,3S)-3-isopropylmalate
mutant enzyme L106M, at pH 7.6 and 25°C
0.0122
(2R,3S)-3-isopropylmalate
-
wild type enzyme, at pH 7.6 and 25°C
0.013
(2R,3S)-3-isopropylmalate
-
pH 7.6, 20°C, stopped flow experiment
0.013
(2R,3S)-3-isopropylmalate
mutant enzyme D217A, at pH 7.6 and 20°C
0.014
(2R,3S)-3-isopropylmalate
mutant enzyme N102A, at pH 7.6 and 20°C
0.0141
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.6 and 25°C
0.015
(2R,3S)-3-isopropylmalate
-
-
0.015
(2R,3S)-3-isopropylmalate
-
pressure 0.1 MPa, 20°C, pH not specified in the publication
0.016
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.6 and 20°C
0.016
(2R,3S)-3-isopropylmalate
wild type enzyme, in the presence of 25 mM K+, at pH 7.6 and 20°C
0.0169
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172D
0.0169
(2R,3S)-3-isopropylmalate
-
mutant enzyme A172D, at 55°C, pH not specified in the publication
0.019
(2R,3S)-3-isopropylmalate
-
pH 9.0
0.02
(2R,3S)-3-isopropylmalate
pressure 100 MPa, 20°C, pH not specified in the publication
0.02
(2R,3S)-3-isopropylmalate
mutant enzyme D245A, at pH 7.6 and 20°C
0.022
(2R,3S)-3-isopropylmalate
60°C, pH 7.6, mutant enzyme A172V
0.0221
(2R,3S)-3-isopropylmalate
-
55°C, wild-type enzyme
0.0221
(2R,3S)-3-isopropylmalate
-
wild type enzyme, at 55°C, pH not specified in the publication
0.0226
(2R,3S)-3-isopropylmalate
mutant enzyme S266A, at pH 7.6 and 25°C
0.0234
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172N
0.0234
(2R,3S)-3-isopropylmalate
-
mutant enzyme A172N, at 55°C, pH not specified in the publication
0.0239
(2R,3S)-3-isopropylmalate
-
mutant enzyme M106L, at pH 7.6 and 25°C
0.024
(2R,3S)-3-isopropylmalate
-
kcat/Km: 88000/Msec
0.0242
(2R,3S)-3-isopropylmalate
mutant enzyme L132A, at pH 7.5 and 25°C
0.025
(2R,3S)-3-isopropylmalate
pressure 0.1 MPa, 20°C, pH not specified in the publication
0.025
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0257
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172E
0.0257
(2R,3S)-3-isopropylmalate
-
mutant enzyme A172E, at 55°C, pH not specified in the publication
0.026
(2R,3S)-3-isopropylmalate
-
pH 9.0
0.0267
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172Q
0.0267
(2R,3S)-3-isopropylmalate
-
mutant enzyme A172Q, at 55°C, pH not specified in the publication
0.027
(2R,3S)-3-isopropylmalate
-
pressure 50 MPa, 20°C, pH not specified in the publication
0.0278
(2R,3S)-3-isopropylmalate
mutant enzyme D292N, at pH 7.5 and 25°C
0.028
(2R,3S)-3-isopropylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
0.0303
(2R,3S)-3-isopropylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
0.031
(2R,3S)-3-isopropylmalate
60°C, pH 7.6, wild-type enzyme
0.032
(2R,3S)-3-isopropylmalate
pressure 150 MPa, 20°C, pH not specified in the publication
0.032
(2R,3S)-3-isopropylmalate
mutant enzyme E270A, at pH 7.6 and 20°C
0.032
(2R,3S)-3-isopropylmalate
mutant enzyme E270A, in the presence of 25 mM K+, at pH 7.6 and 20°C
0.0344
(2R,3S)-3-isopropylmalate
-
mutant enzyme A266S, at pH 7.6 and 25°C
0.0345
(2R,3S)-3-isopropylmalate
mutant enzyme A268I, at pH 7.6 and 25°C
0.0356
(2R,3S)-3-isopropylmalate
-
mutant enzyme I268A, at pH 7.6 and 25°C
0.038
(2R,3S)-3-isopropylmalate
pressure 200 MPa, 20°C, pH not specified in the publication
0.04
(2R,3S)-3-isopropylmalate
mutant enzyme Y139A, at pH 7.6 and 20°C
0.043
(2R,3S)-3-isopropylmalate
pressure 50 MPa, 20°C, pH not specified in the publication
0.053
(2R,3S)-3-isopropylmalate
-
pressure 150 MPa, 20°C, pH not specified in the publication
0.055
(2R,3S)-3-isopropylmalate
-
pressure 200 MPa, 20°C, pH not specified in the publication
0.0707
(2R,3S)-3-isopropylmalate
mutant enzyme R146A, at pH 7.5 and 25°C
0.092
(2R,3S)-3-isopropylmalate
-
pressure 250 MPa, 20°C, pH not specified in the publication
0.108
(2R,3S)-3-isopropylmalate
pressure 250 MPa, 20°C, pH not specified in the publication
0.119
(2R,3S)-3-isopropylmalate
mutant enzyme R146K, at pH 7.5 and 25°C
0.125
(2R,3S)-3-isopropylmalate
mutant enzyme L133A, at pH 7.5 and 25°C
0.145
(2R,3S)-3-isopropylmalate
mutant enzyme K185A, at pH 7.6 and 20°C
0.255
(2R,3S)-3-isopropylmalate
mutant enzyme D241A, at pH 7.6 and 20°C
0.59
(2R,3S)-3-isopropylmalate
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
0.753
(2R,3S)-3-isopropylmalate
mutant enzyme R174K, at pH 7.5 and 25°C
0.9
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
0.9
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C
1
(2R,3S)-3-isopropylmalate
mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1
(2R,3S)-3-isopropylmalate
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C
1
(2R,3S)-3-isopropylmalate
wild-type HICDH, pH 8.0, 37°C
1.5
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C
1.9
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M, pH 8.0, 37°C
2.6
(2R,3S)-3-isopropylmalate
mutant R85V/Y86A, pH 8.0, 37°C
3.73
(2R,3S)-3-isopropylmalate
mutant enzyme R136K, at pH 7.5 and 25°C
0.045
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.077
3-(2'-methylthio)ethylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
0.103
3-(2'-methylthio)ethylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
0.323
3-(2'-methylthio)ethylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
0.436
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.502
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0039
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S/S232G
0.0044
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant L288P
0.0055
3-isopropylmalate
pH 7.5, 70°C, recombinant wild-type enzyme
0.0067
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S/L288P
0.0076
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S
0.008
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant R284G
0.009
3-isopropylmalate
-
wild-type
0.0122
3-isopropylmalate
-
mutant Glu253Leu
0.016
3-isopropylmalate
-
mutant Glu253Ala
0.0189
3-isopropylmalate
-
mutant Glu253Gln
0.0244
3-isopropylmalate
-
mutant Glu253Val
0.0254
3-isopropylmalate
-
mutant Glu253Trp
0.301
D-malate
-
pH 7.5, 37°C
0.41
D-malate
-
kcat/Km: 14000/Msec
0.002
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126G
0.0026
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126A
0.0027
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126T
0.0032
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126S
0.0044
DL-3-isopropylmalate
-
pH 8.0, 60°C, wild-type
0.005
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126M
0.0061
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126E
0.0084
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126I
0.0121
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126L
0.0133
DL-3-isopropylmalate
-
pH 8.0, 60°C, mutant V126F
0.000017
isopropylmalate
-
pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme
0.00048
isopropylmalate
-
pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G
0.001
isopropylmalate
-
pH 8.0, 40°C, mutant enzyme C275T
0.0012
isopropylmalate
-
pH 8.0, 40°C, mutant enzyme G376A
0.00125
isopropylmalate
-
pH 8.0, 40°C, wild-type enzyme
0.0013
isopropylmalate
-
pH 8.0, 40°C, mutant enzyme A31G/G43A/A709G
0.0024
isopropylmalate
-
pH 8.0, 40°C, mutant enzyme G43A
0.00298
isopropylmalate
-
pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme
0.0053
isopropylmalate
-
pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87Q
0.0078
isopropylmalate
-
pH 8.0, 70°C, mutant enzyme C275T
0.0089
isopropylmalate
-
pH 8.0, 70°C, wild-type enzyme
0.0096
isopropylmalate
-
pH 8.0, 70°C, mutant enzyme A31G/G43A/A709G
0.01
isopropylmalate
-
pH 8.0, 70°C, mutant enzyme G376A
0.011
isopropylmalate
-
pH 8.0, 70°C, mutant enzyme G43A
0.044
isopropylmalate
-
pH 7.6, 60°C
0.105
isopropylmalate
-
pH 7.6, 40°C
0.316
isopropylmalate
-
pH 7.6, 40°C
0.0326
malate
-
pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q
1.51
malate
-
pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme
31.56
malate
-
pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme
35.59
malate
-
pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87G
0.0022
NAD+
pH 8.0, 25°C, wild-type enzyme
0.00321
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.0091
NAD+
-
pH 8.0, 40°C, mutant V126S
0.0092
NAD+
-
pH 8.0, 40°C, mutant V126A
0.012
NAD+
pH 8.0, 40°C, wild-type enzyme
0.012
NAD+
-
pH 7.3, 21°C, wild-type enzyme
0.0149
NAD+
-
pH 8.0, 40°C, reaction with 3-D-isopropylmalate, mutant enzyme C275T
0.022
NAD+
-
pH 8.0, 40°C, mutant V126T
0.024
NAD+
-
pH 8.0, 40°C, mutant V126G
0.025
NAD+
-
pH 8.0, 40°C, wild-type
0.0258
NAD+
-
pH 8.0, 40°C, isopropylmalate dehydrogenase wild-type
0.031
NAD+
-
pH 7.3, 21°C, mutant enzyme S226R
0.037
NAD+
-
acIPMDH-ML, pH 8.0, 25°C
0.045
NAD+
-
ancIPMDH-IQ, pH 8.0, 25°C
0.0454
NAD+
-
pH 7.3, 21°C, reaction with tartrate, wild-type enzym
0.0515
NAD+
-
pH 8.0, 40°C, mutant V126E
0.054
NAD+
-
acIPMDH-ML, pH 8.0, 40°C
0.059
NAD+
-
pH 8.0, 60°C, mutant V126A
0.06
NAD+
mutant enzyme Y139A, at pH 7.6 and 20°C
0.062
NAD+
-
pH 8.0, 40°C, mutant V126I
0.064
NAD+
mutant enzyme K185A, at pH 7.6 and 20°C
0.067
NAD+
-
ancIPMDH-IQ, pH 8.0, 40°C
0.0708
NAD+
mutant enzyme L106M, at pH 7.6 and 25°C
0.0725
NAD+
-
pH 8.0, 60°C, mutant V126G
0.0756
NAD+
mutant enzyme S266A, at pH 7.6 and 25°C
0.076
NAD+
-
55°C, mutant enzyme A172D
0.079
NAD+
-
mutant enzyme M106L, at pH 7.6 and 25°C
0.0814
NAD+
wild type enzyme, at pH 7.6 and 25°C
0.094
NAD+
-
55°C, mutant enzyme A172Q
0.095
NAD+
-
pH 8.0, 60°C, mutant V126S
0.1
NAD+
-
55°C, mutant enzyme A172E
0.103
NAD+
-
pH 8.0, 60°C, mutant V126T
0.105
NAD+
-
55°C, mutant enzyme A172N
0.1058
NAD+
-
wild type enzyme, at pH 7.6 and 25°C
0.1065
NAD+
-
pH 7.3, 21°C, reaction with malate, wild-type enzyme
0.109
NAD+
-
pH 8.0, 60°C, mutant V126E
0.111
NAD+
-
pH 8.0, 40°C, mutant V126L
0.116
NAD+
-
55°C, wild-type enzyme
0.12
NAD+
at pH 8.0 and 60°C
0.13
NAD+
60°C, pH 7.6, pH 7.6, mutant enzyme A172V
0.131
NAD+
-
pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G
0.139
NAD+
-
pH 7.6, 60°C
0.14
NAD+
60°C, pH 7.6, wild-type enzyme
0.144
NAD+
-
pH 8.0, 40°C, mutant V126F
0.157
NAD+
-
pH 8.0, 40°C, mutant enzyme A31G/G43A/A709G
0.157
NAD+
-
pH 7.6, 40°C
0.16
NAD+
-
pH 8.0, 40°C, mutant enzyme G43A
0.166
NAD+
-
pH 8.0, 60°C, wild-type
0.1707
NAD+
-
mutant enzyme I268A, at pH 7.6 and 25°C
0.171
NAD+
-
mutant Glu253Val
0.172
NAD+
pH 7.6, 70°C, recombinant mutant L134N
0.181
NAD+
-
pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q
0.198
NAD+
-
pH 8.0, 60°C, mutant V126I
0.21
NAD+
pH 8.0, 70°C, wild-type enzyme
0.22
NAD+
pH 7.5, 70°C, recombinant mutant F3S/S232G
0.225
NAD+
-
pH 8.0, 40°C, isopropylmalate dehydrogenase cold-adapted mutant T1155
0.2379
NAD+
-
mutant enzyme A266S, at pH 7.6 and 25°C
0.244
NAD+
-
mutant Glu253Trp
0.246
NAD+
-
mutant Glu253Ala
0.25
NAD+
pH 7.5, 70°C, recombinant mutant R284G
0.25
NAD+
-
pH 7.6, 20°C, stopped flow experiment
0.255
NAD+
pH 7.6, 70°C, recombinant mutant L134N/V181T/P324T/A335E
0.26
NAD+
mutant enzyme N102A, at pH 7.6 and 20°C
0.273
NAD+
-
mutant Glu253Gln
0.2785
NAD+
mutant enzyme A268I, at pH 7.6 and 25°C
0.29
NAD+
wild type enzyme, at pH 7.6 and 20°C
0.29
NAD+
wild type enzyme, in the presence of 25 mM K+, at pH 7.6 and 20°C
0.305
NAD+
mutant enzyme D217A, at pH 7.6 and 20°C
0.32
NAD+
mutant enzyme D241A, at pH 7.6 and 20°C
0.321
NAD+
-
pH 7.6, 40°C
0.336
NAD+
pH 7.6, 70°C, recombinant mutant H197K
0.341
NAD+
-
pH 8.0, 70°C, isopropylmalate wild-type
0.359
NAD+
pH 7.6, 70°C, recombinant mutant A335E
0.37
NAD+
pH 7.5, 70°C, recombinant mutant L288P
0.371
NAD+
pH 7.6, 70°C, recombinant mutant P324T
0.375
NAD+
mutant enzyme D245A, at pH 7.6 and 20°C
0.378
NAD+
-
pH 8.0, 60°C, mutant V126F
0.386
NAD+
-
pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87G
0.39
NAD+
pH 7.5, 70°C, recombinant wild-type enzyme and mutant F3S
0.391
NAD+
-
pH 8.0, 60°C, mutant V126L
0.4
NAD+
pH 7.5, 70°C, recombinant mutant F3S/L288P
0.401
NAD+
pH 7.6, 70°C, recombinant mutant R58L
0.428
NAD+
pH 7.6, 70°C, recombinant mutant P56E
0.468
NAD+
pH 7.6, 70°C, recombinant mutant F53L
0.474
NAD+
-
mutant Glu253Leu
0.514
NAD+
pH 7.6, 70°C, recombinant wild-type enzyme
0.52
NAD+
pH 7.6, 70°C, recombinant mutant V61I
0.567
NAD+
pH 7.6, 70°C, recombinant mutant V181T/P324T/A335E
0.625
NAD+
pH 7.6, 70°C, recombinant mutant S261N
0.642
NAD+
pH 7.6, 70°C, recombinant mutant V181T
0.66
NAD+
mutant enzyme E270A, at pH 7.6 and 20°C
0.66
NAD+
mutant enzyme E270A, in the presence of 25 mM K+, at pH 7.6 and 20°C
0.673
NAD+
pH 7.6, 70°C, recombinant mutant D184H
0.73
NAD+
-
acIPMDH-ML, pH 8.0, 70°C
0.778
NAD+
-
pH 7.3, 21°C, isopropylmalate dehydrogenase mutant E87Q
0.82
NAD+
-
at 70°C, mutant enzyme G43A
0.9
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C
0.917
NAD+
-
at 70°C, mutant enzyme A31G/G43A/A709G
0.96
NAD+
-
ancIPMDH-IQ, pH 8.0, 70°C
1.01
NAD+
-
at 40°C, isopropylmalate dehydrogenase cold-adapted mutant P215
1.01
NAD+
-
pH 8.0, 40°C, mutant V126M
1.2
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.2
NAD+
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.6
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C
1.6
NAD+
mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.7
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M, pH 8.0, 37°C
1.7
NAD+
mutant R85V/Y86A, pH 8.0, 37°C
1.836
NAD+
-
pH 7.3, 21°C, mutant enzyme S226R/D278K/I279Y
1.853
NAD+
-
pH 7.3, 21°C, reaction with malate, mutant enzyme E87Q
1.9
NAD+
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C
1.9
NAD+
wild-type HICDH, pH 8.0, 37°C
1.97
NAD+
-
pH 8.0, 60°C, mutant V126M
2.584
NAD+
-
pH 7.3, 21°C, reaction with malate, mutant enzyme E87G
3.56
NAD+
-
70°C, isopropylmalate dehydrogenase cold-adapted mutant P215
0.014
NADP+
-
pH 7.3, 21°C, mutant enzyme S226R/D278K/I279Y
0.02
NADP+
-
pH 7.3, 21°C, mutant enzyme D278K, I279T, P325Y and 395-Arg
0.722
NADP+
-
pH 7.3, 21°C, mutant enzyme S226R
1.573
NADP+
-
pH 7.3, 21°C, reaction with tartrate, wild-type enzyme
1.75
NADP+
-
wild-type enzyme
1.836
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
6.579
NADP+
-
pH 7.3, 21°C, recation with malate, wild-type enzyme
0.0407
Tartrate
-
pH 7.3, 21°C, cofactor: NAD+, wild-type enzyme
0.761
Tartrate
-
pH 7.3, 21°C, cofactor: NAD+, isopropylmalate dehydrogenase mutant E87Q
0.817
Tartrate
-
pH 7.3, 21°C, cofactor: NADP+, wild-type enzyme
1.21
Tartrate
-
pH 7.3, 21°C, cofactor: NAD+, mutant enzyme E87G
0.0061
threo-D,L-isopropylmalate
pH 7.6, 70°C, recombinant wild-type enzyme
0.0063
threo-D,L-isopropylmalate
pH 7.6, 70°C, recombinant mutant V181T/P324T/A335E
0.007
threo-D,L-isopropylmalate
pH 7.6, 70°C, recombinant mutant L134N
0.0161
threo-D,L-isopropylmalate
pH 7.6, 70°C, recombinant mutant L134N/V181T/P324T/A335E
additional information
additional information
-
the Km-value for NAD+ ranges from 0.054 mM to 0.15 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml, the Km-value for 3-isopropylmalate ranges from 0.023 mM to 0.042 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml
-
additional information
additional information
-
the Km-value for NAD+ ranges from 0.054 mM to 0.15 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml, the Km-value for 3-isopropylmalate ranges from 0.023 mM to 0.042 mM when enzyme concentration varies from 0.008 mg/ml to 0.16 mg/ml
-
additional information
additional information
Michaelis-Menten kinetics of recombinant wild-type and mutant enzymes at different temperatures, overview
-
additional information
additional information
-
Michaelis-Menten kinetics of recombinant wild-type and mutant enzymes at different temperatures, overview
-
additional information
additional information
-
thermodynamic and kinetics analysis, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0025 - 220
(2R,3S)-3-isopropylmalate
0.016 - 0.85
3-(2'-methylthio)ethylmalate
3.8 - 14.9
3-isopropylmalate
2.7
3-tert-butylmalate
-
-
3.1
D-3-ethylmalate
pH 8.0
3.1
D-3-isobutylmalate
pH 8.0
2.7
D-3-isopentylmalate
pH 8.0
3.6
D-3-isopropylmalate
pH 8.0
3.5
D-3-methylmalate
pH 8.0
0.96
D-3-t-butylmalate
pH 8.0
0.41
D-malate
-
pH 7.5, 37°C
0.26
homoisocitrate
at pH 8.0 and 60°C
0.09 - 69
isopropylmalate
6
tert-butylmalate
-
pH 7.6
additional information
additional information
-
the cold adaption results from the destabilization of the ternary complex caused by increase in the volume of the residue at position 126
-
0.0025
(2R,3S)-3-isopropylmalate
mutant enzyme D241A, at pH 7.6 and 20°C
0.0025
(2R,3S)-3-isopropylmalate
mutant enzyme K185A, at pH 7.6 and 20°C
0.003
(2R,3S)-3-isopropylmalate
mutant enzyme D292N, at pH 7.5 and 25°C
0.017
(2R,3S)-3-isopropylmalate
mutant enzyme Y181A, at pH 7.5 and 25°C
0.023
(2R,3S)-3-isopropylmalate
mutant enzyme R146K, at pH 7.5 and 25°C
0.03
(2R,3S)-3-isopropylmalate
mutant enzyme R146A, at pH 7.5 and 25°C
0.033
(2R,3S)-3-isopropylmalate
mutant enzyme E270A, at pH 7.6 and 20°C
0.033
(2R,3S)-3-isopropylmalate
mutant enzyme E270A, in the presence of K+, at pH 7.6 and 20°C
0.033
(2R,3S)-3-isopropylmalate
mutant enzyme R136K, at pH 7.5 and 25°C
0.045
(2R,3S)-3-isopropylmalate
mutant enzyme D217A, at pH 7.6 and 20°C
0.08
(2R,3S)-3-isopropylmalate
wild-type HICDH, pH 8.0, 37°C
0.108
(2R,3S)-3-isopropylmalate
mutant enzyme Y139A, at pH 7.6 and 20°C
0.233
(2R,3S)-3-isopropylmalate
mutant enzyme Y181F, at pH 7.5 and 25°C
0.3
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C
0.4
(2R,3S)-3-isopropylmalate
mutant R85V/Y86A, pH 8.0, 37°C
0.433
(2R,3S)-3-isopropylmalate
mutant enzyme D245A, at pH 7.6 and 20°C
0.47
(2R,3S)-3-isopropylmalate
mutant enzyme L132A, at pH 7.5 and 25°C
0.55
(2R,3S)-3-isopropylmalate
mutant enzyme N102A, at pH 7.6 and 20°C
0.583
(2R,3S)-3-isopropylmalate
mutant enzyme Y181H, at pH 7.5 and 25°C
0.6
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C
0.62
(2R,3S)-3-isopropylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
0.622
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.73
(2R,3S)-3-isopropylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
1.2
(2R,3S)-3-isopropylmalate
-
acIPMDH-ML, pH 8.0, 25°C
1.6
(2R,3S)-3-isopropylmalate
mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.62
(2R,3S)-3-isopropylmalate
mutant enzyme R174K, at pH 7.5 and 25°C
1.7
(2R,3S)-3-isopropylmalate
-
ancIPMDH-IQ, pH 8.0, 25°C
1.8
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M, pH 8.0, 37°C
2
(2R,3S)-3-isopropylmalate
mutant enzyme V235A, at pH 7.5 and 25°C
2.36
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, wild-type enzyme
2.41
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, mutant enzyme C275T
2.9
(2R,3S)-3-isopropylmalate
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
2.9
(2R,3S)-3-isopropylmalate
-
pH 7.6, 20°C, quenched flow experiment
3.1
(2R,3S)-3-isopropylmalate
-
pressure 250 MPa, 20°C, pH not specified in the publication
3.1
(2R,3S)-3-isopropylmalate
mutant enzyme L133A, at pH 7.5 and 25°C
3.4
(2R,3S)-3-isopropylmalate
-
pH 7.6, 20°C, stopped flow experiment
3.83
(2R,3S)-3-isopropylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
3.97
(2R,3S)-3-isopropylmalate
wild type enzyme, in the presence of K+, at pH 7.6 and 20°C
4.3
(2R,3S)-3-isopropylmalate
-
acIPMDH-ML, pH 8.0, 40°C
4.57
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.5 and 25°C
4.6
(2R,3S)-3-isopropylmalate
-
ancIPMDH-IQ, pH 8.0, 40°C
5.1
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
5.7
(2R,3S)-3-isopropylmalate
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C
5.8
(2R,3S)-3-isopropylmalate
-
pressure 200 MPa, 20°C, pH not specified in the publication
6.22
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
7.2
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, mutant enzyme A31G/G43A/A709G
7.6
(2R,3S)-3-isopropylmalate
-
pressure 100 MPa, 20°C, pH not specified in the publication
8.4
(2R,3S)-3-isopropylmalate
-
pressure 150 MPa, 20°C, pH not specified in the publication
8.5
(2R,3S)-3-isopropylmalate
at pH 8.0 and 60°C
9.05
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
9.6
(2R,3S)-3-isopropylmalate
-
mutant enzyme I268A, at pH 7.6 and 25°C
10
(2R,3S)-3-isopropylmalate
pressure 250 MPa, 20°C, pH not specified in the publication
10.7
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, mutant enzyme G43A
12
(2R,3S)-3-isopropylmalate
-
pH 7.5, 37°C
13
(2R,3S)-3-isopropylmalate
pressure 200 MPa, 20°C, pH not specified in the publication
14
(2R,3S)-3-isopropylmalate
-
pressure 0.1 MPa, 20°C, pH not specified in the publication
14
(2R,3S)-3-isopropylmalate
pressure 100 MPa, 20°C, pH not specified in the publication
14
(2R,3S)-3-isopropylmalate
pressure 150 MPa, 20°C, pH not specified in the publication
14.8
(2R,3S)-3-isopropylmalate
-
mutant enzyme M106L, at pH 7.6 and 25°C
15
(2R,3S)-3-isopropylmalate
pressure 0.1 MPa, 20°C, pH not specified in the publication
15
(2R,3S)-3-isopropylmalate
-
pressure 50 MPa, 20°C, pH not specified in the publication
16.1
(2R,3S)-3-isopropylmalate
mutant enzyme A268I, at pH 7.6 and 25°C
16.4
(2R,3S)-3-isopropylmalate
-
wild type enzyme, at pH 7.6 and 25°C
16.7
(2R,3S)-3-isopropylmalate
mutant enzyme S266A, at pH 7.6 and 25°C
17
(2R,3S)-3-isopropylmalate
pressure 50 MPa, 20°C, pH not specified in the publication
17.4
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.6 and 25°C
17.7
(2R,3S)-3-isopropylmalate
-
pH 8.0, 40°C, mutant enzyme G376A
20.4
(2R,3S)-3-isopropylmalate
-
mutant enzyme A266S, at pH 7.6 and 25°C
22
(2R,3S)-3-isopropylmalate
-
acIPMDH-ML, pH 8.0, 70°C
22
(2R,3S)-3-isopropylmalate
-
ancIPMDH-IQ, pH 8.0, 70°C
28
(2R,3S)-3-isopropylmalate
-
-
40
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.6 and 20°C
44.7
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, mutant enzyme C275T
45.8
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, wild-type enzyme
48.1
(2R,3S)-3-isopropylmalate
mutant enzyme L106M, at pH 7.6 and 25°C
50.1
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, mutant enzyme A31G/G43A/A709G
63.6
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, mutant enzyme G43A
100
(2R,3S)-3-isopropylmalate
-
pH 8.0, 70°C, mutant enzyme G376A
100
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172D
107
(2R,3S)-3-isopropylmalate
-
55°C, wild-type enzyme
111
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172Q
117
(2R,3S)-3-isopropylmalate
-
55°C, mutant enzyme A172E and A172N
163
(2R,3S)-3-isopropylmalate
60°C, pH 7.6, wild-type enzyme
220
(2R,3S)-3-isopropylmalate
60°C, pH 7.6, mutant enzyme A172V
0.016
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.0167
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.033
3-(2'-methylthio)ethylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
0.27
3-(2'-methylthio)ethylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
0.367
3-(2'-methylthio)ethylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
0.85
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
3.8
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S/S232G
6
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant R284G
6.8
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S
6.9
3-isopropylmalate
pH 7.5, 70°C, recombinant wild-type enzyme
7.5
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant L288P
7.8
3-isopropylmalate
pH 7.5, 70°C, recombinant mutant F3S/L288P
8.3
3-isopropylmalate
-
mutant Glu253Trp
9.5
3-isopropylmalate
-
mutant Glu253Ala
9.9
3-isopropylmalate
-
mutant Glu253Gln
12.4
3-isopropylmalate
-
mutant Glu253Val
13.7
3-isopropylmalate
-
mutant Glu253Leu
14.9
3-isopropylmalate
-
wild-type
0.09
isopropylmalate
-
pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R/D278K/I279Y
0.15
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, wild-type enzyme
0.2
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G
0.21
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q
0.24
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, wild-type enzyme
0.26
isopropylmalate
-
pH 7.3, 21°C, reaction with NADP+, wild-type enzyme
0.48
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R
0.88
isopropylmalate
-
pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R
1.66
isopropylmalate
-
pH 7.3, 21°C, reaction with NADP+, wild-type enzyme
1.91
isopropylmalate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R/D278K/I279Y
8
isopropylmalate
-
pH 7.6, 70°C
19
isopropylmalate
-
pH 7.6, 40°C
69
isopropylmalate
-
pH 7.6, 40°C
0.19
malate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q
0.26
malate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G
4.69
malate
-
pH 7.3, 21°C, reaction with NADP+, wild-type enzyme
10.6
malate
-
pH 7.3, 21°C, reaction with NAD+, wild-type enzyme
0.15
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.19
NAD+
-
pH 7.3, 21°C, reaction with tartrate, wild-type enzyme or reaction with malate, mutant enzyme E87Q
0.2
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87G
0.21
NAD+
-
pH 7.3, 21°C, reaction with tartrate, mutant enzyme E87G
0.21
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme E87Q
0.24
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.26
NAD+
-
pH 7.3, 21°C, reaction with malate, mutant enzyme E87G or reaction with tartrate, mutant enzyme E87Q
0.37
NAD+
pH 8.0, 25°C, wild-type enzyme
0.48
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R
1.2
NAD+
-
pH 8.0, 40°C, mutant V126A
1.2
NAD+
-
acIPMDH-ML, pH 8.0, 25°C
1.5
NAD+
-
pH 8.0, 40°C, mutant V126S
1.7
NAD+
-
pH 8.0, 40°C, mutant V126G
1.7
NAD+
-
ancIPMDH-IQ, pH 8.0, 25°C
1.9
NAD+
-
pH 8.0, 40°C, mutant V126T
1.91
NAD+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y
2 - 3.7
NAD+
-
mutant enzyme A266S, at pH 7.6 and 25°C
2.36
NAD+
-
pH 8.0, 40°C, wild-type enzyme
2.4
NAD+
pH 8.0, 40°C, wild-type enzyme
2.4
NAD+
-
pH 8.0, 40°C, wild-type
2.41
NAD+
-
pH 8.0, 40°C, mutant enzyme C275T
3.5
NAD+
-
pH 8.0, 40°C, mutant V126E
4.3
NAD+
-
acIPMDH-ML, pH 8.0, 40°C
4.6
NAD+
-
ancIPMDH-IQ, pH 8.0, 40°C
6
NAD+
-
pH 8.0, 40°C, mutant V126I
7.2
NAD+
-
pH 8.0, 40°C, mutant enzyme A31G/G43A/A709G
8
NAD+
-
pH 7.6, 70°C, reaction with isopropylmalate
8.5
NAD+
-
pH 8.0, 40°C, mutant V126L
9
NAD+
-
pH 8.0, 60°C, mutant V126A
10
NAD+
-
pH 8.0, 60°C, V126G
10.2
NAD+
pH 7.6, 70°C, recombinant mutant V181T
10.3
NAD+
-
mutant enzyme I268A, at pH 7.6 and 25°C
10.6
NAD+
-
pH 7.3, 21°C, reaction with malate, wild-type enzyme
10.7
NAD+
-
pH 8.0, 40°C, mutant enzyme G43A
11
NAD+
-
pH 8.0, 40°C, mutant V126F
11.2
NAD+
pH 7.6, 70°C, recombinant mutant D184H
13
NAD+
-
pH 8.0, 60°C, mutant V126S
13.2
NAD+
pH 7.6, 70°C, recombinant mutant V181T/P324T/A335E
13.4
NAD+
pH 7.6, 70°C, recombinant wild-type enzyme
13.8
NAD+
pH 7.6, 70°C, recombinant mutant P324T
14.6
NAD+
pH 7.6, 70°C, recombinant mutant P56E
15
NAD+
-
pH 8.0, 60°C, mutant V126T
15
NAD+
pH 7.6, 70°C, recombinant mutants F53L and V61I
15.2
NAD+
-
mutant enzyme M106L, at pH 7.6 and 25°C
15.6
NAD+
mutant enzyme S266A, at pH 7.6 and 25°C
17.1
NAD+
wild type enzyme, at pH 7.6 and 25°C
17.4
NAD+
mutant enzyme A268I, at pH 7.6 and 25°C
17.7
NAD+
-
pH 8.0, 40°C, mutant enzyme G376A
17.7
NAD+
pH 7.6, 70°C, recombinant mutant R58L
18
NAD+
-
pH 8.0, 40°C, mutant V126M
18.2
NAD+
pH 7.6, 70°C, recombinant mutants S261N and A335E
18.7
NAD+
-
wild type enzyme, at pH 7.6 and 25°C
19
NAD+
-
pH 7.6, 40°C, reaction with isopropylmalate
20
NAD+
-
pH 8.0, 60°C, wild-type
21
NAD+
-
pH 8.0, 60°C, mutant V126E
21.3
NAD+
pH 7.6, 70°C, recombinant mutant H197K
22
NAD+
-
acIPMDH-ML, pH 8.0, 70°C
22
NAD+
-
ancIPMDH-IQ, pH 8.0, 70°C
25.2
NAD+
pH 7.6, 70°C, recombinant mutant L134N/V181T/P324T/A335E
29.9
NAD+
pH 7.6, 70°C, recombinant mutant L134N
30.5
NAD+
-
pH 8.0, 60°C, mutant V126I
42
NAD+
-
pH 8.0, 60°C, mutant V126F
44.5
NAD+
-
pH 8.0, 60°C, mutant V126L
44.7
NAD+
-
pH 8.0, 70°C, mutant enzyme C275T
45.8
NAD+
-
pH 8.0, 70°C, wild-type enzyme
50.1
NAD+
-
pH 8.0, 70°C, mutant enzyme A31G/G43A/A709G
50.5
NAD+
mutant enzyme L106M, at pH 7.6 and 25°C
54
NAD+
-
pH 8.0, 60°C, mutant V126M
63.6
NAD+
-
pH 8.0, 70°C, mutant enzyme G43A
69
NAD+
-
pH 7.6, 40°C, reaction with isopropylmalate
79
NAD+
pH 8.0, 70°C, wild-type enzyme
100
NAD+
-
pH 8.0, 70°C, mutant enzyme G376A
100
NAD+
-
55°C, mutant enzyme A172D
107
NAD+
-
55°C, wild-type enzyme
111
NAD+
-
55°C, mutant enzyme A172Q
117
NAD+
-
55°C, mutant enzyme A172E and A172N
163
NAD+
60°C, pH 7.6, wild-type enzyme
220
NAD+
60°C, pH 7.6, pH 7.6, mutant enzyme A172V
0.09
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y
0.1
NADP+
-
pH 7.3, 21°C, reaction with tartrate, wild-type enzyme
0.26
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
0.88
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R
1.66
NADP+
-
pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme
4.69
NADP+
-
pH 7.3, 21°C, reaction with malate, wild-type enzyme
0.1
Tartrate
-
pH 7.3, 21°C, reaction with NADP+, wild-type enzyme
0.19
Tartrate
-
pH 7.3, 21°C, reaction with NAD+, wild-type enzyme
0.21
Tartrate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87G
0.26
Tartrate
-
pH 7.3, 21°C, reaction with NAD+, mutant enzyme E87Q
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.009 - 1700
(2R,3S)-3-isopropylmalate
0.038 - 18.77
3-(2'-methylthio)ethylmalate
1.36
D-malate
-
pH 7.5, 37°C
440
homoisocitrate
at pH 8.0 and 60°C
0.009
(2R,3S)-3-isopropylmalate
mutant enzyme R136K, at pH 7.5 and 25°C
0.109
(2R,3S)-3-isopropylmalate
mutant enzyme D292N, at pH 7.5 and 25°C
0.16
(2R,3S)-3-isopropylmalate
mutant R85V/Y86A, pH 8.0, 37°C
0.18
(2R,3S)-3-isopropylmalate
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C
0.192
(2R,3S)-3-isopropylmalate
mutant enzyme R146K, at pH 7.5 and 25°C
0.33
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C
0.4
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C
0.429
(2R,3S)-3-isopropylmalate
mutant enzyme R146A, at pH 7.5 and 25°C
0.49
(2R,3S)-3-isopropylmalate
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
0.75
(2R,3S)-3-isopropylmalate
wild-type HICDH, pH 8.0, 37°C
0.94
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M, pH 8.0, 37°C
1.6
(2R,3S)-3-isopropylmalate
mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
1.92
(2R,3S)-3-isopropylmalate
mutant enzyme Y181A, at pH 7.5 and 25°C
2.14
(2R,3S)-3-isopropylmalate
mutant enzyme R174K, at pH 7.5 and 25°C
5.4
(2R,3S)-3-isopropylmalate
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
19.29
(2R,3S)-3-isopropylmalate
mutant enzyme L132A, at pH 7.5 and 25°C
20.3
(2R,3S)-3-isopropylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
24.47
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
24.83
(2R,3S)-3-isopropylmalate
mutant enzyme L133A, at pH 7.5 and 25°C
25.7
(2R,3S)-3-isopropylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
32.32
(2R,3S)-3-isopropylmalate
mutant enzyme Y181F, at pH 7.5 and 25°C
34
(2R,3S)-3-isopropylmalate
-
pressure 250 MPa, 20°C, pH not specified in the publication
91.21
(2R,3S)-3-isopropylmalate
mutant enzyme Y181H, at pH 7.5 and 25°C
93
(2R,3S)-3-isopropylmalate
pressure 250 MPa, 20°C, pH not specified in the publication
110
(2R,3S)-3-isopropylmalate
-
pressure 200 MPa, 20°C, pH not specified in the publication
160
(2R,3S)-3-isopropylmalate
-
pressure 150 MPa, 20°C, pH not specified in the publication
180.3
(2R,3S)-3-isopropylmalate
mutant enzyme V235A, at pH 7.5 and 25°C
270
(2R,3S)-3-isopropylmalate
-
mutant enzyme I268A, at pH 7.6 and 25°C
336.3
(2R,3S)-3-isopropylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
340
(2R,3S)-3-isopropylmalate
pressure 200 MPa, 20°C, pH not specified in the publication
400
(2R,3S)-3-isopropylmalate
pressure 50 MPa, 20°C, pH not specified in the publication
440
(2R,3S)-3-isopropylmalate
pressure 150 MPa, 20°C, pH not specified in the publication
560
(2R,3S)-3-isopropylmalate
-
pressure 50 MPa, 20°C, pH not specified in the publication
570
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
590
(2R,3S)-3-isopropylmalate
-
mutant enzyme A266S, at pH 7.6 and 25°C
600
(2R,3S)-3-isopropylmalate
pressure 0.1 MPa, 20°C, pH not specified in the publication
620
(2R,3S)-3-isopropylmalate
-
mutant enzyme M106L, at pH 7.6 and 25°C
700
(2R,3S)-3-isopropylmalate
pressure 100 MPa, 20°C, pH not specified in the publication
870
(2R,3S)-3-isopropylmalate
-
pressure 100 MPa, 20°C, pH not specified in the publication
930
(2R,3S)-3-isopropylmalate
-
pressure 0.1 MPa, 20°C, pH not specified in the publication
983.7
(2R,3S)-3-isopropylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
1150
(2R,3S)-3-isopropylmalate
-
pH 7.5, 37°C
1303
(2R,3S)-3-isopropylmalate
wild type enzyme, at pH 7.5 and 25°C
1350
(2R,3S)-3-isopropylmalate
-
wild type enzyme, at pH 7.6 and 25°C
1700
(2R,3S)-3-isopropylmalate
at pH 8.0 and 60°C
0.038
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.1
3-(2'-methylthio)ethylmalate
mutant F137L, pH not specified in the publication, temperature not specified in the publication
0.33
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
2.59
3-(2'-methylthio)ethylmalate
mutant L134F, pH not specified in the publication, temperature not specified in the publication
4.76
3-(2'-methylthio)ethylmalate
mutant L133F, pH not specified in the publication, temperature not specified in the publication
18.77
3-(2'-methylthio)ethylmalate
wild-type, pH not specified in the publication, temperature not specified in the publication
0.12
NAD+
wild-type (2R,3S)-3-isopropylmalate dehydrogenase, pH 8.0, 37°C
0.23
NAD+
mutant R85V/Y86A, pH 8.0, 37°C
0.25
NAD+
mutant H15Y/E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
0.33
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/V238M/R310M, pH 8.0, 37°C
0.38
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M, pH 8.0, 37°C
0.41
NAD+
wild-type HICDH, pH 8.0, 37°C
1.1
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/R310M , pH 8.0, 37°C
1.4
NAD+
mutant S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
4.2
NAD+
mutant E57V/S72I/R85M/Y86A/M208T/F217Y/V238M/R310M, pH 8.0, 37°C
60
NAD+
-
mutant enzyme I268A, at pH 7.6 and 25°C
100
NAD+
-
mutant enzyme A266S, at pH 7.6 and 25°C
180
NAD+
-
wild type enzyme, at pH 7.6 and 25°C
190
NAD+
-
mutant enzyme M106L, at pH 7.6 and 25°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.