show 
Biochemical characterization of an alcohol dehydrogenase from Ralstonia sp.
Kulig, J.; Frese, A.; Kroutil, W.; Pohl, M.; Rother, D.; Biotechnol. Bioeng. 110, 1838-1848 (2013)
Data extracted from this reference:
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Ralstonia sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
(R)-2-hydroxypropiophenone
-
Ralstonia sp.
Cyclohexanol
-
Ralstonia sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
with benzaldehyde as cosubstrate, at pH 7.5 and 30°C
Ralstonia sp.
0.008
-
NADPH
with cyclohexanone as cosubstrate, at pH 7.5 and 30°C
Ralstonia sp.
0.06
-
NADP+
with benzyl alcohol as cosubstrate, at pH 9.0 and 30°C
Ralstonia sp.
1.6
-
(R)-2-hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
3.6
-
(S)-2-Hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
5.2
-
Cyclohexanol
at pH 9.0 and 30°C
Ralstonia sp.
10.8
-
cyclohexanone
at pH 7.5 and 30°C
Ralstonia sp.
12.3
-
benzyl alcohol
at pH 9.0 and 30°C
Ralstonia sp.
15.6
-
benzaldehyde
at pH 7.5 and 30°C
Ralstonia sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
Ca2+ has an stabilizing effect. With 1.0 mM CaCl2, the enzyme is completely stable at 0°C for 2 h and after 3.5 h almost 90% of the initial activity is retained
Ralstonia sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ralstonia sp.
-
-
-
Ralstonia sp. DSM 6428
-
-
-
Purification (Commentary)
Commentary
Organism
-
Ralstonia sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.01
-
with acetone, at pH 7.5 and 30°C
Ralstonia sp.
0.05
-
with acetaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
0.08
-
with 2,5-hexandione, at pH 7.5 and 30°C
Ralstonia sp.
0.11
-
with cyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
0.56
-
with 4-phenyl-2-butanone, at pH 7.5 and 30°C
Ralstonia sp.
4.35
-
with acetophenone, at pH 7.5 and 30°C
Ralstonia sp.
4.89
-
with benzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
8.36
-
with cyclohexanol, at pH 10.5 and 30°C
Ralstonia sp.
9.78
-
with cyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
11.58
-
with 3-methylcyclohexanol, at pH 10.5 and 30°C
Ralstonia sp.
11.7
-
with 3-fluorobenzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
12.62
-
with 3-methylcyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
17.1
-
with (S)-2-hydroxypropiophenone, at pH 7.5 and 30°C
Ralstonia sp.
18.83
-
with cyclohexenol, at pH 10.5 and 30°C
Ralstonia sp.
23.31
-
2-bromobenzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
362.6
-
with (R)-2-hydroxypropiophenone, at pH 7.5 and 30°C
Ralstonia sp.
Storage Stability
Storage Stability
Organism
-20°C, 10 mM TEA-HCl buffer pH 7.5, at least 80 days, no loss of activity
Ralstonia sp.
8°C, 50 mM TEA-HCl buffer pH 7.5, 1 day, 91% loss of activity
Ralstonia sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(1R)-1-hydroxy-1-phenylpropan-2-one + NADPH + H+
-
740200
Ralstonia sp.
(1R)-1-phenylpropane-1,2-diol + NADP+
-
-
-
?
(2R)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
740200
Ralstonia sp.
(2R)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
-
-
?
(2S)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
740200
Ralstonia sp.
(2S)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
-
-
?
(R)-2-hydroxypropiophenone + NADPH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
(S)-2-hydroxypropiophenone + NADPH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
2,5-hexandione + NADH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
2-bromobenzaldehyde + NADPH
-
740200
Ralstonia sp.
2-bromobenzyl alcohol + NADP+
-
-
-
r
2-bromobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-bromobenzyl alcohol + NADP+
-
-
-
?
2-cyclohexenol + NADP+
-
740200
Ralstonia sp.
2-cyclohexenone + NADH + H+
-
-
-
r
2-cyclohexenone + NADH + H+
-
740200
Ralstonia sp.
2-cyclohexenol + NADP+
-
-
-
r
2-fluorobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-fluorobenzyl alcohol + NADP+
-
-
-
?
2-hydroxy-2-methyl-1-phenylpropan-1-one + NADPH + H+
-
740200
Ralstonia sp.
2-methyl-1-phenylpropan-1,2-diol + NADP+
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-methoxybenzyl alcohol + NADP+
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp. DSM 6428
2-methoxybenzyl alcohol + NADP+
-
-
-
?
2-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-methylbenzyl alcohol + NADP+
-
-
-
?
3-fluorobenzaldehyde + NADPH
-
740200
Ralstonia sp.
3-fluorobenzyl alcohol + NADP+
-
-
-
r
3-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
3-methoxybenzyl alcohol + NADP+
-
-
-
?
3-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
3-methylbenzyl alcohol + NADP+
-
-
-
?
3-methylcyclohexanol + NADP+
-
740200
Ralstonia sp.
?
-
-
-
r
3-methylcyclohexanone + NADH + H+
-
740200
Ralstonia sp.
3-methylcyclohexanol + NAD+
-
-
-
r
4-bromobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-bromobenzyl alcohol + NADP+
-
-
-
?
4-fluorobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-fluorobenzyl alcohol + NADP+
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-methoxybenzyl alcohol + NADP+
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp. DSM 6428
4-methoxybenzyl alcohol + NADP+
-
-
-
?
4-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-methylbenzyl alcohol + NADP+
-
-
-
?
4-methylpentan-2-one + NADPH + H+
-
740200
Ralstonia sp.
4-methylpentan-2-ol + NADP+
-
-
-
?
4-phenyl-2-butanone + NADH + H+
-
740200
Ralstonia sp.
4-phenyl-2-butanol + NAD+
-
-
-
r
acetaldehyde + NADPH
-
740200
Ralstonia sp.
ethanol + NADP+
-
-
-
r
acetone + NADH + H+
-
740200
Ralstonia sp.
propan-2-ol + NAD+
-
-
-
r
acetophenone + NADH + H+
-
740200
Ralstonia sp.
1-phenylethan-1-ol + NAD+
-
-
-
r
benzaldehyde + NADPH
-
740200
Ralstonia sp.
benzyl alcohol + NADP+
-
-
-
r
benzaldehyde + NADPH
-
740200
Ralstonia sp. DSM 6428
benzyl alcohol + NADP+
-
-
-
r
benzyl alcohol + NADP+
-
740200
Ralstonia sp.
benzaldehyde + NADPH
-
-
-
r
benzyl alcohol + NADP+
-
740200
Ralstonia sp. DSM 6428
benzaldehyde + NADPH
-
-
-
r
butan-2-ol + NADP+
-
740200
Ralstonia sp.
butan-2-one + NADPH + H+
-
-
-
?
butan-2-one + NADPH + H+
-
740200
Ralstonia sp.
butan-2-ol + NADP+
-
-
-
?
cyclohexanol + NADP+
-
740200
Ralstonia sp.
cyclohexanone + NADH + H+
-
-
-
r
cyclohexanone + NADH + H+
-
740200
Ralstonia sp.
cyclohexanol + NAD+
-
-
-
r
cyclohexanone + NADH + H+
-
740200
Ralstonia sp.
cyclohexanol + NADP+
-
-
-
r
formaldehyde + NADPH + H+
-
740200
Ralstonia sp.
methanol + NADP+
-
-
-
?
furan-2-carbaldehyde + NADPH + H+
-
740200
Ralstonia sp.
(furan-2-yl)methanol + NADP+
-
-
-
?
heptan-4-one + NADPH + H+
-
740200
Ralstonia sp.
heptan-4-ol + NADP+
-
-
-
?
hexan-1-ol + NADP+
-
740200
Ralstonia sp.
hexaldehyde + NADPH + H+
-
-
-
?
hexan-2-one + NADPH + H+
-
740200
Ralstonia sp.
hexan-2-ol + NADP+
-
-
-
?
hexanal + NADPH + H+
-
740200
Ralstonia sp.
hexan-1-ol + NADP+
-
-
-
?
additional information
no activity with methanol and ethanol
740200
Ralstonia sp.
?
-
-
-
-
additional information
no activity with methanol and ethanol
740200
Ralstonia sp. DSM 6428
?
-
-
-
-
octan-1-ol + NADP+
-
740200
Ralstonia sp.
octaldehyde + NADPH + H+
-
-
-
?
octan-2-one + NADPH + H+
-
740200
Ralstonia sp.
octan-2-ol + NADP+
-
-
-
?
pentan-1-ol + NADP+
-
740200
Ralstonia sp.
pentaldehyde + NADPH + H+
-
-
-
?
propan-1-ol + NADP+
-
740200
Ralstonia sp.
propionaldehyde + NADPH + H+
-
-
-
?
propan-2-ol + NADP+
-
740200
Ralstonia sp.
propan-2-one + NADPH + H+
-
-
-
?
pyridine-2-carbaldehyde + NADPH + H+
-
740200
Ralstonia sp.
(pyridin-2-yl)methanol + NADP+
-
-
-
?
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
8
25
the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h)
Ralstonia sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
9.5
reduction reaction
Ralstonia sp.
10
11.5
oxidation reaction
Ralstonia sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
8
the enzyme shows half-lives of 60-70 h between pH 5.5 and 8.0 at room temperature
Ralstonia sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Ralstonia sp.
NADPH
-
Ralstonia sp.
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
11.4
-
Cyclohexanol
at pH 9.0 and 30°C
Ralstonia sp.
16.4
-
(R)-2-hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Ralstonia sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Ralstonia sp.
NADPH
-
Ralstonia sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(R)-2-hydroxypropiophenone
-
Ralstonia sp.
Cyclohexanol
-
Ralstonia sp.
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
11.4
-
Cyclohexanol
at pH 9.0 and 30°C
Ralstonia sp.
16.4
-
(R)-2-hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
with benzaldehyde as cosubstrate, at pH 7.5 and 30°C
Ralstonia sp.
0.008
-
NADPH
with cyclohexanone as cosubstrate, at pH 7.5 and 30°C
Ralstonia sp.
0.06
-
NADP+
with benzyl alcohol as cosubstrate, at pH 9.0 and 30°C
Ralstonia sp.
1.6
-
(R)-2-hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
3.6
-
(S)-2-Hydroxypropiophenone
at pH 7.5 and 30°C
Ralstonia sp.
5.2
-
Cyclohexanol
at pH 9.0 and 30°C
Ralstonia sp.
10.8
-
cyclohexanone
at pH 7.5 and 30°C
Ralstonia sp.
12.3
-
benzyl alcohol
at pH 9.0 and 30°C
Ralstonia sp.
15.6
-
benzaldehyde
at pH 7.5 and 30°C
Ralstonia sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
Ca2+ has an stabilizing effect. With 1.0 mM CaCl2, the enzyme is completely stable at 0°C for 2 h and after 3.5 h almost 90% of the initial activity is retained
Ralstonia sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Ralstonia sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.01
-
with acetone, at pH 7.5 and 30°C
Ralstonia sp.
0.05
-
with acetaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
0.08
-
with 2,5-hexandione, at pH 7.5 and 30°C
Ralstonia sp.
0.11
-
with cyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
0.56
-
with 4-phenyl-2-butanone, at pH 7.5 and 30°C
Ralstonia sp.
4.35
-
with acetophenone, at pH 7.5 and 30°C
Ralstonia sp.
4.89
-
with benzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
8.36
-
with cyclohexanol, at pH 10.5 and 30°C
Ralstonia sp.
9.78
-
with cyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
11.58
-
with 3-methylcyclohexanol, at pH 10.5 and 30°C
Ralstonia sp.
11.7
-
with 3-fluorobenzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
12.62
-
with 3-methylcyclohexanone, at pH 7.5 and 30°C
Ralstonia sp.
17.1
-
with (S)-2-hydroxypropiophenone, at pH 7.5 and 30°C
Ralstonia sp.
18.83
-
with cyclohexenol, at pH 10.5 and 30°C
Ralstonia sp.
23.31
-
2-bromobenzaldehyde, at pH 7.5 and 30°C
Ralstonia sp.
362.6
-
with (R)-2-hydroxypropiophenone, at pH 7.5 and 30°C
Ralstonia sp.
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 10 mM TEA-HCl buffer pH 7.5, at least 80 days, no loss of activity
Ralstonia sp.
8°C, 50 mM TEA-HCl buffer pH 7.5, 1 day, 91% loss of activity
Ralstonia sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(1R)-1-hydroxy-1-phenylpropan-2-one + NADPH + H+
-
740200
Ralstonia sp.
(1R)-1-phenylpropane-1,2-diol + NADP+
-
-
-
?
(2R)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
740200
Ralstonia sp.
(2R)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
-
-
?
(2S)-2-hydroxy-1-(4-methoxyphenyl)propan-1-one + NADPH + H+
-
740200
Ralstonia sp.
(2S)-1-(4-methoxyphenyl)propane-1,2-diol + NADP+
-
-
-
?
(R)-2-hydroxypropiophenone + NADPH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
(S)-2-hydroxypropiophenone + NADPH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
2,5-hexandione + NADH + H+
-
740200
Ralstonia sp.
?
-
-
-
r
2-bromobenzaldehyde + NADPH
-
740200
Ralstonia sp.
2-bromobenzyl alcohol + NADP+
-
-
-
r
2-bromobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-bromobenzyl alcohol + NADP+
-
-
-
?
2-cyclohexenol + NADP+
-
740200
Ralstonia sp.
2-cyclohexenone + NADH + H+
-
-
-
r
2-cyclohexenone + NADH + H+
-
740200
Ralstonia sp.
2-cyclohexenol + NADP+
-
-
-
r
2-fluorobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-fluorobenzyl alcohol + NADP+
-
-
-
?
2-hydroxy-2-methyl-1-phenylpropan-1-one + NADPH + H+
-
740200
Ralstonia sp.
2-methyl-1-phenylpropan-1,2-diol + NADP+
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-methoxybenzyl alcohol + NADP+
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp. DSM 6428
2-methoxybenzyl alcohol + NADP+
-
-
-
?
2-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
2-methylbenzyl alcohol + NADP+
-
-
-
?
3-fluorobenzaldehyde + NADPH
-
740200
Ralstonia sp.
3-fluorobenzyl alcohol + NADP+
-
-
-
r
3-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
3-methoxybenzyl alcohol + NADP+
-
-
-
?
3-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
3-methylbenzyl alcohol + NADP+
-
-
-
?
3-methylcyclohexanol + NADP+
-
740200
Ralstonia sp.
?
-
-
-
r
3-methylcyclohexanone + NADH + H+
-
740200
Ralstonia sp.
3-methylcyclohexanol + NAD+
-
-
-
r
4-bromobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-bromobenzyl alcohol + NADP+
-
-
-
?
4-fluorobenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-fluorobenzyl alcohol + NADP+
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-methoxybenzyl alcohol + NADP+
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
-
740200
Ralstonia sp. DSM 6428
4-methoxybenzyl alcohol + NADP+
-
-
-
?
4-methylbenzaldehyde + NADPH + H+
-
740200
Ralstonia sp.
4-methylbenzyl alcohol + NADP+
-
-
-
?
4-methylpentan-2-one + NADPH + H+
-
740200
Ralstonia sp.
4-methylpentan-2-ol + NADP+
-
-
-
?
4-phenyl-2-butanone + NADH + H+
-
740200
Ralstonia sp.
4-phenyl-2-butanol + NAD+
-
-
-
r
acetaldehyde + NADPH
-
740200
Ralstonia sp.
ethanol + NADP+
-
-
-
r
acetone + NADH + H+
-
740200
Ralstonia sp.
propan-2-ol + NAD+
-
-
-
r
acetophenone + NADH + H+
-
740200
Ralstonia sp.
1-phenylethan-1-ol + NAD+
-
-
-
r
benzaldehyde + NADPH
-
740200
Ralstonia sp.
benzyl alcohol + NADP+
-
-
-
r
benzaldehyde + NADPH
-
740200
Ralstonia sp. DSM 6428
benzyl alcohol + NADP+
-
-
-
r
benzyl alcohol + NADP+
-
740200
Ralstonia sp.
benzaldehyde + NADPH
-
-
-
r
benzyl alcohol + NADP+
-
740200
Ralstonia sp. DSM 6428
benzaldehyde + NADPH
-
-
-
r
butan-2-ol + NADP+
-
740200
Ralstonia sp.
butan-2-one + NADPH + H+
-
-
-
?
butan-2-one + NADPH + H+
-
740200
Ralstonia sp.
butan-2-ol + NADP+
-
-
-
?
cyclohexanol + NADP+
-
740200
Ralstonia sp.
cyclohexanone + NADH + H+
-
-
-
r
cyclohexanone + NADH + H+
-
740200
Ralstonia sp.
cyclohexanol + NAD+
-
-
-
r
cyclohexanone + NADH + H+
-
740200
Ralstonia sp.
cyclohexanol + NADP+
-
-
-
r
formaldehyde + NADPH + H+
-
740200
Ralstonia sp.
methanol + NADP+
-
-
-
?
furan-2-carbaldehyde + NADPH + H+
-
740200
Ralstonia sp.
(furan-2-yl)methanol + NADP+
-
-
-
?
heptan-4-one + NADPH + H+
-
740200
Ralstonia sp.
heptan-4-ol + NADP+
-
-
-
?
hexan-1-ol + NADP+
-
740200
Ralstonia sp.
hexaldehyde + NADPH + H+
-
-
-
?
hexan-2-one + NADPH + H+
-
740200
Ralstonia sp.
hexan-2-ol + NADP+
-
-
-
?
hexanal + NADPH + H+
-
740200
Ralstonia sp.
hexan-1-ol + NADP+
-
-
-
?
additional information
no activity with methanol and ethanol
740200
Ralstonia sp.
?
-
-
-
-
additional information
no activity with methanol and ethanol
740200
Ralstonia sp. DSM 6428
?
-
-
-
-
octan-1-ol + NADP+
-
740200
Ralstonia sp.
octaldehyde + NADPH + H+
-
-
-
?
octan-2-one + NADPH + H+
-
740200
Ralstonia sp.
octan-2-ol + NADP+
-
-
-
?
pentan-1-ol + NADP+
-
740200
Ralstonia sp.
pentaldehyde + NADPH + H+
-
-
-
?
propan-1-ol + NADP+
-
740200
Ralstonia sp.
propionaldehyde + NADPH + H+
-
-
-
?
propan-2-ol + NADP+
-
740200
Ralstonia sp.
propan-2-one + NADPH + H+
-
-
-
?
pyridine-2-carbaldehyde + NADPH + H+
-
740200
Ralstonia sp.
(pyridin-2-yl)methanol + NADP+
-
-
-
?
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
8
25
the enzyme has a half-life of 80 h at 25°C and stability optimum between 8 and 15°C in the presence of 0.8 mM CaCl2 (half-life of 130 h)
Ralstonia sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
9.5
reduction reaction
Ralstonia sp.
10
11.5
oxidation reaction
Ralstonia sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
8
the enzyme shows half-lives of 60-70 h between pH 5.5 and 8.0 at room temperature
Ralstonia sp.
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739830
Holec
-
P450 BM3 monooxygenase as an e ...
Equus caballus, Ralstonia sp., Thermoanaerobacter brockii
Adv. Synth. Catal.
358
1810-1819
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738540
Zheng
Cofactor specificity of the bi ...
Hungateiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
4
4
740291
Lundova
Molecular and biochemical char ...
Homo sapiens
Chem. Biol. Interact.
234
178-187
2015
-
-
1
-
-
-
-
-
2
-
1
1
-
3
-
-
1
-
-
6
-
-
8
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
1
1
-
-
-
1
-
6
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741095
Brummund
-
Process Development for biocat ...
Rhodococcus ruber
Org. Process Res. Dev.
19
1590-1595
2015
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
737536
Elleuche
Group III alcohol dehydrogenas ...
Pectobacterium atrosepticum, Pectobacterium atrosepticum DSM18077
Appl. Microbiol. Biotechnol.
98
4041-4051
2014
-
-
1
-
5
-
4
-
-
1
2
-
-
2
-
-
1
-
-
-
-
-
15
1
1
1
1
-
1
1
-
1
-
1
-
-
-
1
1
-
5
-
-
4
-
-
-
1
2
-
-
-
-
1
-
-
-
-
15
1
1
1
1
-
1
1
-
1
-
-
-
-
-
-
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
-
-
1
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium salinarum NRC 1, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
-
-
2
-
-
-
-
10
-
3
1
-
8
4
-
-
1
-
-
-
-
2
26
1
1
-
-
-
4
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
10
-
3
1
-
8
-
-
1
-
-
-
2
26
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
735486
Wu
Thermostable alcohol dehydroge ...
Thermococcus kodakarensis
Appl. Environ. Microbiol.
79
2209-2217
2013
-
-
1
-
-
-
6
9
-
-
3
48
10
1
-
-
1
-
-
-
-
-
48
1
1
-
1
9
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
6
-
9
-
-
3
48
10
-
-
1
-
-
-
-
48
1
1
-
1
9
2
-
-
-
-
-
-
-
9
9
740200
Kulig
Biochemical characterization o ...
Ralstonia sp., Ralstonia sp. DSM 6428
Biotechnol. Bioeng.
110
1838-1848
2013
-
-
1
-
-
-
2
9
-
1
-
-
-
4
-
-
1
-
-
-
16
2
53
-
-
-
1
-
2
-
1
2
2
-
-
-
-
1
2
-
-
-
-
2
2
9
-
1
-
-
-
-
-
1
-
-
16
2
53
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
740851
Ma
Characterization and cofactor ...
Klebsiella pneumoniae, Klebsiella pneumoniae DSM2026
J. Microbiol. Biotechnol.
23
1699-1707
2013
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
12
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
-
-
1
-
-
-
-
3
-
1
1
-
3
4
-
-
1
-
-
-
-
1
8
1
1
1
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
3
-
-
1
-
-
-
1
8
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
-
-
1
-
-
1
-
5
-
1
2
-
4
2
-
-
1
-
-
-
-
2
14
1
1
1
-
-
2
-
1
2
-
-
-
-
-
1
2
-
-
1
-
-
-
5
-
1
2
-
4
-
-
1
-
-
-
2
14
1
1
1
-
-
2
-
1
-
-
-
-
-
5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Hungateiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
-
-
1
-
-
-
14
5
-
3
3
-
-
7
-
-
1
-
-
-
-
-
30
1
1
-
4
5
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
14
-
5
-
3
3
-
-
-
-
1
-
-
-
-
30
1
1
-
4
5
2
-
-
-
-
-
-
-
5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
2
-
1
-
-
-
-
5
-
-
-
1
-
1
-
-
1
1
-
1
-
-
15
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
-
-
1
-
-
-
-
6
2
-
1
-
-
1
-
-
-
1
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
2
-
1
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
-
-
-
-
-
-
6
17
-
-
2
-
-
20
-
-
2
2
-
-
-
-
35
2
-
-
-
13
4
2
-
10
-
-
-
-
-
-
10
-
-
-
-
6
-
17
-
-
2
-
-
-
-
2
-
-
-
-
35
2
-
-
-
13
4
2
-
-
-
-
-
-
-
-
287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
-
-
-
-
-
-
2
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
1
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287280
Hatanaka
-
Purification and properties of ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides IFO 3426
Agric. Biol. Chem.
38
1819-1833
1974
-
-
-
-
-
-
9
8
-
-
-
-
-
2
-
-
1
-
-
-
-
-
14
-
-
-
1
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
9
-
8
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
287281
Rhodes
-
Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
J. Biol. Chem.
243
4372-4379
1968
2
-
-
-
-
-
5
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
14
-
-
-
-
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-