KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.2 | - |
5-Dehydro-D-gluconate | pH 7.0, temperature not specified in the publication | Aspergillus niger | |
3.2 | - |
5-Dehydro-D-gluconate | pH 7, temperature not specified in the publication | Aspergillus niger | |
8.4 | - |
D-gluconate | pH 8.0, temperature not specified in the publication | Aspergillus niger | |
8.4 | - |
D-gluconate | pH 8, temperature not specified in the publication | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | G3XRE5 | - |
- |
Aspergillus niger ATCC 1015 | G3XRE5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-dehydro-D-gluconate + NADPH + H+ | - |
Aspergillus niger | D-gluconate + NADP+ | - |
r | |
5-dehydro-D-gluconate + NADPH + H+ | - |
Aspergillus niger ATCC 1015 | D-gluconate + NADP+ | - |
r | |
D-gluconate + NADP+ | - |
Aspergillus niger | 5-dehydro-D-gluconate + NADPH + H+ | - |
r | |
D-gluconate + NADP+ | - |
Aspergillus niger ATCC 1015 | 5-dehydro-D-gluconate + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
5-keto-D-gluconate reductase | - |
Aspergillus niger |
GluF | - |
Aspergillus niger |
NADPH-dependent 5-keto-D-gluconate reductase | - |
Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 3.7 | 5-Dehydro-D-gluconate | pH 7.0, temperature not specified in the publication | Aspergillus niger | |
2 | 3.7 | 5-Dehydro-D-gluconate | pH 7, temperature not specified in the publication | Aspergillus niger | |
2.6 | - |
D-gluconate | pH 8.0, temperature not specified in the publication | Aspergillus niger | |
2.6 | - |
D-gluconate | pH 8, temperature not specified in the publication | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NAD+/NADH | Aspergillus niger | |
NADP+ | - |
Aspergillus niger | |
NADPH | - |
Aspergillus niger |
General Information | Comment | Organism |
---|---|---|
malfunction | the disruption of the gene, gluF, by CRISPR/Cas9 in the filamentous fungus Aspergillus niger results in a strain unable to catabolise D-glucuronate | Aspergillus niger |
metabolism | the enzyme is involved in the pathway for D-glucuronate catabolism in fungi | Aspergillus niger |
physiological function | gene disruption results in a strain unable to catabolise D-glucuronate. A pathway for D-glucuronate catabolism is suggested with the intermediates L-gulonate, 2-keto-L-gulonate, L-idonate, 5-keto-D-gluconate, D-gluconate and D-gluconate-6-phosphate | Aspergillus niger |