BRENDA - Enzyme Database show
show all sequences of 1.1.1.362

Cloning, sequencing, and analysis of aklaviketone reductase from Streptomyces sp. strain C5

Dickens, M.L.; Ye, J.; Strohl, W.R.; J. Bacteriol. 178, 3384-3388 (1996)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
extracts of Streptomyces lividans TK24 containing recombinant DauE catalyzes the NADPH-specific conversion of aklaviketone, maggiemycin, and 7-oxodaunomycinone to aklavinone, epsilon-rhodomycinone, and daunomycinone, respectively
Streptomyces sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25632
-
x * 25632, calculated from sequence
Streptomyces sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
aklaviketone + NADPH + H+
Streptomyces sp.
the enzyme is involved in the biosynthesis of the anthracyclins aclacinomycin and daunomycin. Aklavinone is the last intermediate common to both aclacinomycin and daunomycin biosynthesis
aklavinone + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces sp.
Q53882
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-oxodaunomycinone + NADPH + H+
-
722491
Streptomyces sp.
daunomycinone + NADP+
-
-
-
?
aklaviketone + NADPH + H+
the enzyme is involved in the biosynthesis of the anthracyclins aclacinomycin and daunomycin. Aklavinone is the last intermediate common to both aclacinomycin and daunomycin biosynthesis
722491
Streptomyces sp.
aklavinone + NADP+
-
-
-
?
aklaviketone + NADPH + H+
activity with NADH is 7% of the activity with NADPH
722491
Streptomyces sp.
aklavinone + NADP+
-
-
-
?
maggiemycin + NADPH + H+
-
722491
Streptomyces sp.
epsilon-rhodomycinone + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 25632, calculated from sequence
Streptomyces sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
activity with NADH is 7% of the activity with NADPH
Streptomyces sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
extracts of Streptomyces lividans TK24 containing recombinant DauE catalyzes the NADPH-specific conversion of aklaviketone, maggiemycin, and 7-oxodaunomycinone to aklavinone, epsilon-rhodomycinone, and daunomycinone, respectively
Streptomyces sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
activity with NADH is 7% of the activity with NADPH
Streptomyces sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25632
-
x * 25632, calculated from sequence
Streptomyces sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
aklaviketone + NADPH + H+
Streptomyces sp.
the enzyme is involved in the biosynthesis of the anthracyclins aclacinomycin and daunomycin. Aklavinone is the last intermediate common to both aclacinomycin and daunomycin biosynthesis
aklavinone + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-oxodaunomycinone + NADPH + H+
-
722491
Streptomyces sp.
daunomycinone + NADP+
-
-
-
?
aklaviketone + NADPH + H+
the enzyme is involved in the biosynthesis of the anthracyclins aclacinomycin and daunomycin. Aklavinone is the last intermediate common to both aclacinomycin and daunomycin biosynthesis
722491
Streptomyces sp.
aklavinone + NADP+
-
-
-
?
aklaviketone + NADPH + H+
activity with NADH is 7% of the activity with NADPH
722491
Streptomyces sp.
aklavinone + NADP+
-
-
-
?
maggiemycin + NADPH + H+
-
722491
Streptomyces sp.
epsilon-rhodomycinone + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 25632, calculated from sequence
Streptomyces sp.
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of the anthracyclins aclacinomycin and daunomycin
Streptomyces sp.
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in the biosynthesis of the anthracyclins aclacinomycin and daunomycin
Streptomyces sp.
Other publictions for EC 1.1.1.362
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
722491
Dickens
Cloning, sequencing, and analy ...
Streptomyces sp.
J. Bacteriol.
178
3384-3388
1996
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