Application | Comment | Organism |
---|---|---|
synthesis | establishing of an enzyme-catalyzed synthesis system for production of poly(3-hydroxybutyrate) in vitro on the basis of the poly(3-hydroxybutyrate) biosynthesis pathway of Ralstonia eutropha, recycling CoA for synthesis of acetyl-CoA and deriving NADPH from regeneration by GDH, overview | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
gene phaB, expression of the N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Cupriavidus necator |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | competitive inhibition | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADPH + H+ | step in the enzyme-catalyzed synthesis system for production of poly(3-hydroxybutyrate) in vitro, overview | Cupriavidus necator | (R)-3-hydroxybutyryl-CoA + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent acetoacetyl-CoA reductase | - |
Cupriavidus necator |
PhaB | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | assay at | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Cupriavidus necator |