Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Candida parapsilosis |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M Tris-HCl pH 8.1 and 23% (w/v) PEG 3350 | Candida parapsilosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Candida parapsilosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydropantolactone + NADPH + H+ | Candida parapsilosis | - |
(R)-pantolactone + NADP+ | - |
? | |
2-dehydropantolactone + NADPH + H+ | Candida parapsilosis IFO 0708 | - |
(R)-pantolactone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | Q76L36 | - |
- |
Candida parapsilosis IFO 0708 | Q76L36 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose column chromatography and Superdex 75 gel filtration | Candida parapsilosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydropantolactone + NADPH + H+ | - |
Candida parapsilosis | (R)-pantolactone + NADP+ | - |
? | |
2-dehydropantolactone + NADPH + H+ | - |
Candida parapsilosis IFO 0708 | (R)-pantolactone + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Candida parapsilosis |
Synonyms | Comment | Organism |
---|---|---|
conjugated polyketone reductase C2 | - |
Candida parapsilosis |
CPR-C2 | - |
Candida parapsilosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Candida parapsilosis |