Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Lacticaseibacillus paracasei |
Crystallization (Comment) | Organism |
---|---|
hexagonal crystals of recombinant D-HicDH in the presence of NAD+ and 2-oxoisocaproate (4-methyl-2-oxopentanoate) are grown with ammonium sulfate as precipitating agent. The structure of the crystals is solved by molecular replacement and refined to a final R-factor of 19.6% for all measured X-ray reflections in the resolution range 1.9 A resolution | Lacticaseibacillus paracasei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
2 * 37000, SDS-PAGE | Lacticaseibacillus paracasei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lacticaseibacillus paracasei | P17584 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | very low specificity regarding size and chemical constitution of the accepted D-2-hydroxycarboxylates | Lacticaseibacillus paracasei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37000, SDS-PAGE | Lacticaseibacillus paracasei |
Synonyms | Comment | Organism |
---|---|---|
D-2-hydroxyisocaproate dehydrogenase | - |
Lacticaseibacillus paracasei |
D-HicDH | - |
Lacticaseibacillus paracasei |