Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces avermitilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0065 | - |
1-deoxy-11beta-hydroxypentalenic acid | pH 8.0, 22°C | Streptomyces avermitilis | |
0.025 | - |
NAD+ | pH 8.0, 22°C | Streptomyces avermitilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30183 | - |
x * 30183, MALDI-TOF, recombinant protein including His-tag | Streptomyces avermitilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces avermitilis | Q82IY9 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-deoxy-11beta-hydroxypentalenic acid + NAD+ | - |
Streptomyces avermitilis | 1-deoxy-11-oxopentalenic acid + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30183, MALDI-TOF, recombinant protein including His-tag | Streptomyces avermitilis |
Synonyms | Comment | Organism |
---|---|---|
ptlF | - |
Streptomyces avermitilis |
SAV_2933 | locus name | Streptomyces avermitilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
1-deoxy-11beta-hydroxypentalenic acid | pH 8.0, 22°C | Streptomyces avermitilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
and second maximum at pH 11.3 | Streptomyces avermitilis |
11.3 | - |
activity increases from pH 9.0 to pH 11.3, and is 12fold higher at pH 11.3 than at pH 8.0 | Streptomyces avermitilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | 150fold preference for NAD+ over NADP+ | Streptomyces avermitilis | |
NADP+ | 150fold preference for NAD+ over NADP+ | Streptomyces avermitilis |