BRENDA - Enzyme Database show
show all sequences of 1.1.1.315

cDNA cloning, tissue distribution, and substrate characteristics of a cis-Retinol/3alpha-hydroxysterol short-chain dehydrogenase isozyme.

Su, J.; Chai, X.; Kahn, B.; Napoli, J.L.; J. Biol. Chem. 273, 17910-17916 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in CHO cells
Mus musculus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cooperative kinetics
Mus musculus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mus musculus
O88451
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
intense expression of CRAD2 mRNA
Mus musculus
-
lung
expression of CRAD2 mRNA is 3% of that in liver
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-cis-retinol + NAD+
activity with NAD+ is about 10fold higher than with NADP+
692942
Mus musculus
11-cis-retinal + NADH + H+
-
-
-
?
11-cis-retinol + NADP+
activity with NAD+ is about 10fold higher than with NADP+
692942
Mus musculus
11-cis-retinal + NADPH + H+
-
-
-
?
13-cis-retinol + NAD+
-
692942
Mus musculus
13-cis-retinal + NADH + H+
-
-
-
?
9-cis-retinol + NAD+
activity with NAD+ is about 8fold higher than with NADP+
692942
Mus musculus
9-cis-retinal + NADH + H+
-
-
-
?
9-cis-retinol + NADP+
activity with NAD+ is about 8fold higher than with NADP+
692942
Mus musculus
9-cis-retinal + NADPH + H+
-
-
-
?
all-trans-retinol + NAD+
-
692942
Mus musculus
all-trans-retinal + NADH + H+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
activity with NAD+ is about 10fold higher than with NADP+
Mus musculus
NADP+
activity with NAD+ is about 10fold higher than with NADP+
Mus musculus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in CHO cells
Mus musculus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
activity with NAD+ is about 10fold higher than with NADP+
Mus musculus
NADP+
activity with NAD+ is about 10fold higher than with NADP+
Mus musculus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cooperative kinetics
Mus musculus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
intense expression of CRAD2 mRNA
Mus musculus
-
lung
expression of CRAD2 mRNA is 3% of that in liver
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-cis-retinol + NAD+
activity with NAD+ is about 10fold higher than with NADP+
692942
Mus musculus
11-cis-retinal + NADH + H+
-
-
-
?
11-cis-retinol + NADP+
activity with NAD+ is about 10fold higher than with NADP+
692942
Mus musculus
11-cis-retinal + NADPH + H+
-
-
-
?
13-cis-retinol + NAD+
-
692942
Mus musculus
13-cis-retinal + NADH + H+
-
-
-
?
9-cis-retinol + NAD+
activity with NAD+ is about 8fold higher than with NADP+
692942
Mus musculus
9-cis-retinal + NADH + H+
-
-
-
?
9-cis-retinol + NADP+
activity with NAD+ is about 8fold higher than with NADP+
692942
Mus musculus
9-cis-retinal + NADPH + H+
-
-
-
?
all-trans-retinol + NAD+
-
692942
Mus musculus
all-trans-retinal + NADH + H+
-
-
-
?
Other publictions for EC 1.1.1.315
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740740
Sahu
Conditional ablation of retino ...
Homo sapiens, Mus musculus
J. Biol. Chem.
290
27239-27247
2015
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
724922
Parker
Retinol dehydrogenases (RDHs) ...
Mus musculus
Exp. Eye Res.
91
788-792
2010
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
690842
Takahashi
Characterization of key residu ...
Homo sapiens
Biochem. J.
419
113-122
2009
-
-
1
-
13
-
-
8
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
4
-
13
-
-
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692705
Farjo
RDH10 has 11-cis-retinol dehyd ...
Bos taurus, Homo sapiens
Invest. Ophthalmol. Vis. Sci.
50
5089-5097
2009
1
-
1
-
-
-
-
2
-
-
-
2
-
5
-
-
-
-
-
1
1
-
6
1
-
-
-
-
-
-
-
2
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
1
1
-
6
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
687735
Belyaeva
Kinetic analysis of human enzy ...
Homo sapiens
J. Biol. Chem.
283
20299-20308
2008
-
-
1
-
-
-
3
6
-
-
1
1
-
2
-
-
1
-
-
-
-
1
9
1
-
-
-
-
-
-
-
2
-
-
-
-
-
2
3
-
-
-
-
3
-
9
-
-
1
1
-
-
-
1
-
-
-
1
9
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692332
Picozzi
Genomic organization and trans ...
Homo sapiens
FEBS Lett.
554
59-66
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715461
Wu
Mapping the ligand binding poc ...
Homo sapiens
J. Biol. Chem.
278
12390-12396
2003
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692700
Wu
Cloning and characterization o ...
Bos taurus, Homo sapiens, Mus musculus
Invest. Ophthalmol. Vis. Sci.
43
3365-3372
2002
-
-
3
-
-
-
-
-
3
-
2
-
-
6
-
-
-
-
-
9
-
-
9
1
-
-
-
-
-
-
-
12
-
1
-
-
-
3
12
-
-
-
-
-
-
-
3
-
2
-
-
-
-
-
-
9
-
-
9
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
715451
Chen
Interaction of 11-cis-retinol ...
Bos taurus
J. Biol. Chem.
276
21098-21104
2001
-
-
-
-
-
-
-
-
1
-
1
3
-
2
-
-
1
-
-
2
-
-
3
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
1
3
-
-
-
1
-
2
-
-
3
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
715455
Liden
Biochemical defects in 11-cis- ...
Homo sapiens
J. Biol. Chem.
276
49251-49257
2001
-
1
1
1
11
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
1
11
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716054
Huang
Modulation of the androgenic r ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
77
129-133
2001
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
690797
Wang
Activity of human 11-cis-retin ...
Homo sapiens
Biochem. J.
338
23-27
1999
-
-
1
-
-
-
-
3
-
-
-
2
-
3
-
-
-
-
-
45
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
3
-
-
-
2
-
-
-
-
-
49
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715709
Simon
Intracellular localization and ...
Bos taurus
J. Cell Sci.
112
549-558
1999
-
-
-
-
2
-
-
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716292
Gonzalez-Fernandez
-
11-cis Retinol dehydrogenase m ...
Homo sapiens
Mol. Vis.
5
XI-XII
1999
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716309
Yamamoto
Mutations in the gene encoding ...
Homo sapiens
Nat. Genet.
22
188-191
1999
-
1
1
-
2
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
170681
Mata
Distribution of 11-cis LRAT, 1 ...
Bos taurus
Biochim. Biophys. Acta
1394
16-22
1998
-
-
-
-
-
-
-
-
3
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692942
Su
cDNA cloning, tissue distribut ...
Mus musculus
J. Biol. Chem.
273
17910-17916
1998
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
692939
Chai
cDNA cloning and characterizat ...
Mus musculus
J. Biol. Chem.
272
33125-33131
1997
-
-
1
-
-
-
1
1
-
-
-
1
-
2
-
-
-
-
-
5
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
5
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
714012
Tsigelny
Structures important in NAD(P) ...
Bos taurus
Biochem. Biophys. Res. Commun.
226
118-127
1996
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
715421
Simon
The retinal pigment epithelial ...
Bos taurus
J. Biol. Chem.
270
1107-1112
1995
-
-
1
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
1
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-