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Literature summary for 1.1.1.219 extracted from

  • Katsu, K.; Suzuki, R.; Tsuchiya, W.; Inagaki, N.; Yamazaki, T.; Hisano, T.; Yasui, Y.; Komori, T.; Koshio, M.; Kubota, S.; Walker, A.R.; Furukawa, K.; Matsui, K.
    A new buckwheat dihydroflavonol 4-reductase (DFR), with a unique substrate binding structure, has altered substrate specificity (2017), BMC Plant Biol., 17, 239 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.219

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Fagopyrum esculentum subsp. esculentum

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular docking of substrates. A phenol ring of dihydrokaempferol, a catechol ring of dihydroquercetin, and a pyrogallol ring of dihydromyricetin respectively have two, three, and four hydrogen bonds formed with side chains of residue Asn132 Fagopyrum esculentum subsp. esculentum
molecular docking of substrates. The number of hydrogen bonds is one, two, and two for the corresponding phenol, catechol, and pyrogallol rings, respectively Fagopyrum esculentum subsp. esculentum

Organism

Organism UniProt Comment Textmining
Fagopyrum esculentum subsp. esculentum A0A2Z5XER5 isoform DFR1a
-
Fagopyrum esculentum subsp. esculentum A0A2Z5XEV7 isoform DFR2
-

Source Tissue

Source Tissue Comment Organism Textmining
bud high expression level Fagopyrum esculentum subsp. esculentum
-
additional information expression in all organs investigated Fagopyrum esculentum subsp. esculentum
-
root high expression level Fagopyrum esculentum subsp. esculentum
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root preferential expression in root and seed Fagopyrum esculentum subsp. esculentum
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seed preferential expression in root and seed Fagopyrum esculentum subsp. esculentum
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stem high expression level in lower stem Fagopyrum esculentum subsp. esculentum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-dihydrokaempferol + NADPH + H+
-
 Fagopyrum esculentum subsp. esculentum cis-3,4-leucopelargonidin + NADP+
-
 ?
(+)-dihydromyricetin + NADPH + H+
-
 Fagopyrum esculentum subsp. esculentum cis-3,4-leucodelphinidin + NADP+
-
 ?
(+)-dihydroquercetin + NADPH + H+
-
 Fagopyrum esculentum subsp. esculentum cis-3,4-leucocyanidin + NADP+
-
 ?
additional information isoform DFR1a catalyses dihydromyricetin and dihydrokaempferol with almost the same efficiency, but catalyses dihydroquercetin with a lower efficiency Fagopyrum esculentum subsp. esculentum ?
-
-
additional information isoform FeDFR2 catalyses dihydroquercetin about 2 times as efficiently as dihydromyricetin and had least activity for dihydrokaempferol Fagopyrum esculentum subsp. esculentum ?
-
-

Synonyms

Synonyms Comment Organism
DFR1a
-
 Fagopyrum esculentum subsp. esculentum
DFR2
-
 Fagopyrum esculentum subsp. esculentum