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Literature summary for 1.1.1.202 extracted from
- Structural and biochemical characterisation of a NAD+-dependent alcohol dehydrogenase from Oenococcus oeni as a new model molecule for industrial biotechnology applications (2013), Appl. Microbiol. Biotechnol., 97, 8963-8975.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Oenococcus oeni |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| to 3.2 A resolution. The electron density map around the active site shows no sign of a bound co-factor, but the active site metal could be located and identified as Ni2+ | Oenococcus oeni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| imidazole | activity is reduced down to 32% in the presence of 1 mM imidazole and addition of 250 mM results in complete inactivity | Oenococcus oeni |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
NAD+ | pH 9.0, 60°C | Oenococcus oeni | |
| 0.2 | - |
NADH | pH 8.0, 60°C | Oenococcus oeni | |
| 11.5 | - |
1,2-propanediol | pH 9.0, 60°C | Oenococcus oeni | |
| 26.7 | - |
1,3-Propanediol | pH 9.0, 60°C | Oenococcus oeni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | located near active site, crystallization data. The activity with 1,3-propanediol is highly dependent on the presence of Ni2+ | Oenococcus oeni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42200 | - |
2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein | Oenococcus oeni |
| 43000 | - |
2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein | Oenococcus oeni |
| 74000 | - |
gel filtration | Oenococcus oeni |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oenococcus oeni | A0NIJ1 | - |
- |
| Oenococcus oeni ATCC BAA-1163 | A0NIJ1 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.1 | - |
pH 9.0, 60°C | Oenococcus oeni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol + NAD+ | - |
Oenococcus oeni | 2-hydroxypropanal + NADH | - |
? | |
| 1,2-propanediol + NAD+ | - |
Oenococcus oeni ATCC BAA-1163 | 2-hydroxypropanal + NADH | - |
? | |
| 1,3-propanediol + NAD+ | activity with 1,3-propanediol is highly dependent on the presence of Ni2+ | Oenococcus oeni | 3-hydroxypropanal + NADH | - |
? | |
| 1,3-propanediol + NAD+ | activity with 1,3-propanediol is highly dependent on the presence of Ni2+ | Oenococcus oeni ATCC BAA-1163 | 3-hydroxypropanal + NADH | - |
? | |
| additional information | enzyme additionally accepts ethanol, 1-propanol, 2-mercaptoethanol and their reduces their corresponding aldehydes. No substrates: methanol, 1-butanol, glycerol or 2-propanol | Oenococcus oeni | ? | - |
? | |
| additional information | enzyme additionally accepts ethanol, 1-propanol, 2-mercaptoethanol and their reduces their corresponding aldehydes. No substrates: methanol, 1-butanol, glycerol or 2-propanol | Oenococcus oeni ATCC BAA-1163 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 42200, calculated, 2 * 43000, SDS-PAGE of recombinant His-tagged protein | Oenococcus oeni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ADH3 | - |
Oenococcus oeni |
| DhaT | - |
Oenococcus oeni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Oenococcus oeni |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
60 min, inactivation | Oenococcus oeni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
reduction of aldehydes | Oenococcus oeni |
| 9 | - |
oxidation of alcohols | Oenococcus oeni |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | most stable within this range | Oenococcus oeni |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no cofactor: NADPH | Oenococcus oeni | |
| NAD+ | - |
Oenococcus oeni | |
| NADH | - |
Oenococcus oeni | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Oenococcus oeni | calculated | - |
5.2 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
1,2-propanediol | pH 9.0, 60°C | Oenococcus oeni | |
| 0.01 | - |
1,3-Propanediol | pH 9.0, 60°C | Oenococcus oeni | |
| 4.9 | - |
NAD+ | pH 9.0, 60°C | Oenococcus oeni | |
| 12.9 | - |
NADH | pH 8.0, 60°C | Oenococcus oeni | |
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Release 2023.1 (January 2023)
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