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Literature summary for 1.1.1.202 extracted from

  • Marcal, D.; Rego, A.T.; Carrondo, M.A.; Enguita, F.J.
    1,3-Propanediol dehydrogenase from Klebsiella pneumoniae: decameric quaternary structure and possible subunit cooperativity (2009), J. Bacteriol., 191, 1143-1151.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.202

Cloned(Commentary)

Cloned (Comment) Organism
the dhaT gene coding for 1,3-PD dehydrogenase inserted into vector pET-YSBLIC and transformed into Escherichia coli BL21(DE3) Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
by vapor diffusion in sitting drops at 20°C, at 2.7 A resolution. The enzyme shows a decameric structure, formed by a pentamer of dimers, which is the catalytic form of the enzyme. Dimers are associated by strong ionic interactions that are responsible for the highly stable in vivo packing of the enzyme. The decameric arrangement is related to the cooperativity between monomers Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
EDTA 50 mM is necessary to obtain a 50% inhibition of enzymatic activity Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16.7
-
 Propane-1,3-diol
-
 Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
415000
-
 sequence analysis Klebsiella pneumoniae
446000
-
 dynamic light scattering analysis of the purified protein in solution Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae Q7WRJ3
-
-

Purification (Commentary)

Purification (Comment) Organism
by centrifugation, nickel affinity column and gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
propane-1,3-diol + NAD+ + H+
-
 Klebsiella pneumoniae 3-hydroxypropanal + NADH
-
 ?

Subunits

Subunits Comment Organism
decamer crystallography or gel filtration Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
1,3-PD dehydrogenase
-
 Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Klebsiella pneumoniae