Application | Comment | Organism |
---|---|---|
synthesis | recombinant borneol dehydrogenase is found in inclusion bodies when expressed in Escherichia coli. Changing the medium from lysogeny broth to Terrific Broth yield a soluble form of the enzyme | Pseudomonas sp. TCU-HL1 |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas sp. TCU-HL1 |
expression in Escherichia coli | Pseudomonas sp. TCU-HL1 |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 30% (w/v) PEG 8000, 0.1 M sodium acetate pH 4.5, 0.2 M lithium sulfate | Pseudomonas sp. TCU-HL1 |
to 1.84 A resolution. Protein features a seven-stranded antiparallel beta-sheet flanked by three alpha-helices on each side | Pseudomonas sp. TCU-HL1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | Pseudomonas sp. TCU-HL1 | - |
(+)-camphor + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. TCU-HL1 | A0A1B3EB36 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate saturation, phenyl Sepharose column chromatography, and Q Sepharose column chromatography | Pseudomonas sp. TCU-HL1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | - |
Pseudomonas sp. TCU-HL1 | (+)-camphor + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Pseudomonas sp. TCU-HL1 |
BDH1 | - |
Pseudomonas sp. TCU-HL1 |
borneol dehydrogenase | - |
Pseudomonas sp. TCU-HL1 |
THL1_4180 | - |
Pseudomonas sp. TCU-HL1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. TCU-HL1 |