Information on EC 1.1.1.198 - (+)-borneol dehydrogenase

Substrates: substrate specificity, overview. No activity with other monoterpene alcohol, several kinds of monoterpene alcohols including (-)-artemisia alcohol, cis-carveol, trans-carveol, and trans-pinocarveol. The enzyme is specific for (+)-borneol
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additional information

Substrates: no substrate: 1,8-cineole, citronellol, linalool, lavandulol, nerol, geraniol, perillyl alcohol, and farnesol. The reverse reduction assay in which camphor is used as a substrate and NADH as a cofactor, does not produce detectable amounts of borneol or other products
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additional information

additional information

Substrates: (-)-borneol is an additional substrate, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate
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additional information

Substrates: the recombinant enzyme does not use isopropanol, cyclohexanol, cyclopentanol, or(+/-)-2-butanol as alternative substrates, and NADP+ as a cofactor
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additional information

Pseudomonas monteilii TCU-CK1

Substrates: (-)-borneol is an additional substrate, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate
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additional information

Pseudomonas monteilii TCU-CK1

Substrates: the recombinant enzyme does not use isopropanol, cyclohexanol, cyclopentanol, or(+/-)-2-butanol as alternative substrates, and NADP+ as a cofactor
Products: -

additional information

Pseudomonas putida ATCC 17453

Substrates: enzyme also catalyzes the reaction of (-)-borneol and (-)-camphor, reaction of EC 1.1.1.227. No substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, 2-butanol, L-carveol, and DL-menthol
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additional information

additional information

Substrates: GC-MS analysis of borneol degradation metabolites
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additional information

Substrates: GC-MS analysis of borneol degradation metabolites
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additional information

Substrates: the enzyme is also active with (-)-borneol, reaction of EC 1.1.1.227
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additional information

Substrates: the enzyme is also active with (-)-borneol, reaction of EC 1.1.1.227
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additional information

Pseudomonas sp. ATCC 17453

Substrates: isopropanol, cyclopentanol, cyclohexanol, 1,2-butandiol, (+/-)-2-butanol, L-carveol, and DL-menthol are not accepted as substrates
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additional information

Substrates: enzyme additionally catalyzes the reaction with (-)-borneol, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate
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additional information

Substrates: enzyme additionally catalyzes the reaction with (-)-borneol, reaction of EC 1.1.1.227. (+)-Borneol is the preferred substrate
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additional information

Substrates: no activity with menthol
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additional information

Substrates: isoforms BDH1 and BDH2 also accept monocyclic and linear substrates, clearly favoring secondary alcohols over primary alcohols. No substrate: menthol
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additional information

Salvia rosmarinus

Substrates: no activity with menthol
Products: -

show all columns Go to Natural Substrate Search

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

(+)-borneol + NAD+

(+)-camphor + NADH

(+)-borneol + NAD+

(+)-camphor + NADH + H+

9 entries

(+)-camphor + NADH + H+

(+)-borneol + NAD+

Pseudomonas sp. ATCC 17453

Substrates: -
Products: -

(+/-)-isoborneol + NAD+

(-)-camphor + NADH + H+

Substrates: -
Products: -

additional information

(+)-borneol + NAD+

(+)-camphor + NADH

286162

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH

Substrates: B-site stereospecific, partially reversible
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

Pseudomonas sp. ATCC 17453

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

760303

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

760892, 762321

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

Substrates: -
Products: -

(+)-borneol + NAD+

(+)-camphor + NADH + H+

Salvia rosmarinus

Substrates: -
Products: -

additional information

Substrates: GC-MS analysis of borneol degradation metabolites
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additional information

Substrates: GC-MS analysis of borneol degradation metabolites
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show all columns Go to Cofactor Search

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

NAD+

12 entries

NADP+

4 entries

additional information

6 entries

NAD+

286162, 286163, 762115

NAD+

NAD+

NAD+

NAD+

NAD+

NAD+

760303

NAD+

NAD+

760892, 762321

NAD+

NAD+

Pseudomonas sp. ATCC 17453

NAD+

strong preference for NAD+ over NADP+ is observed. When cofactor NAD+ is replaced by NADP+, the catalytic rate of refolded BDH is reduced to below 5% compared to NAD+. NAD+ docking into the active site of the BDH homology model for binding domain and mode analysis

NADP+

low activity

NADP+

25% as efficient as NAD+

NADP+

65-85% as efficient as NAD+

286162

NADP+

strong preference for NAD+ over NADP+ is observed. When cofactor NAD+ is replaced by NADP+, the catalytic rate of refolded BDH is reduced to below 5% compared to NAD+

additional information

no cofactor: NADP+

additional information

no cofactor: NADP+

additional information

E5DD06

no cofactor: NADP+

726116

additional information

an N-terminal conserved Gly13-X-X-X-Gly17-X-Gly19 sequence motif is present in the NAD(H)-binding region

additional information

an N-terminal conserved Gly13-X-X-X-Gly17-X-Gly19 sequence motif is present in the NAD(H)-binding region

additional information

enzyme AaBDH is active in the presence of cofactor NADP+ or NAD+, NAD+ is the preferred cofactor

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

(+)-Camphor

3 entries

(-)-Camphor

(-)-Thujol

competitive inhibition of (+)-borneol oxidation

8-hydroxyquinoline

57% inhibition at 1 mM

Borate

inhibition of borneol oxidation

Cu2+

85% inhibition at 2 mM

ethylenediaminetetraacetate

slight inhibition at 1 mM

Hg2+

nearly complete inhibition at 2 mM

iodoacetamide

41% inhibition at 1 mM

N-ethylmaleimide

53% inhibition at 1 mM

NaCN

slight inhibition at 1 mM

NADH

46% inhibition at 1 mM

NaN3

slight inhibition at 1 mM

o-phenanthroline

slight inhibition at 1 mM

p-hydroxymercuribenzoate

50% inhibition at 0.05 mM

Pb2+

26% inhibition at 2 mM

(+)-Camphor

(+)-Camphor

(+)-Camphor

27% inhibition at 0.5 mM

(-)-Camphor

(-)-Camphor

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0251 - 0.46

(+)-borneol

21 entries

0.086

(-)-artemisia alcohol

E5DD06

pH 9.5, 30°C

726116

0.0369 - 1.5

(-)-borneol

9 entries

0.027

(-)-cis-carveol

E5DD06

pH 9.5, 30°C

726116

0.041

(-)-Thujol

0.006 - 0.07

NAD+

4 entries

additional information

Michaelis-Menten kinetics

0.0251

(+)-borneol

isoform BDH3, at pH 8.5 and 30°C

0.03

(+)-borneol

0.0483

(+)-borneol

pH 9.0, 33°C, recombinant enzyme

0.053

(+)-borneol

pH 8.0, 32°C

0.07

(+)-borneol

isoform BDH2, at pH 7.0, temperature not specified in the publication

0.09

(+)-borneol

soluble isoform BDH1, at pH 7.0, temperature not specified in the publication

0.09

(+)-borneol

soluble recombinant protein, pH 7, 25°C

0.095

(+)-borneol

wild-type, pH 7.5, 30°C

0.095

(+)-borneol

Pseudomonas sp. ATCC 17453

wild type enzyme, at pH 7.5 and 30°C

0.12

(+)-borneol

refolded isoform BDH1, at pH 7.0, temperature not specified in the publication

0.12

(+)-borneol

refolded recombinant protein, pH 7, 25°C

0.13

(+)-borneol

at pH 11.0 and 25°C

0.13

(+)-borneol

pH 11, 25°C

0.14

(+)-borneol

pH 7, 25°C

0.14

(+)-borneol

at pH 7.0 and 25°C

0.16

(+)-borneol

isoform BDH2, at pH 8.0 and 20°C

0.2

(+)-borneol

recombinant enzyme, pH 8.5, 22°C

0.28

(+)-borneol

Pseudomonas sp. ATCC 17453

mutant enzyme S146A/Y188A, at pH 7.5 and 30°C

0.28

(+)-borneol

mutant Y188A, pH 7.5, 30°C

0.46

(+)-borneol

Pseudomonas sp. ATCC 17453

mutant enzyme S146A/Y188T, at pH 7.5 and 30°C

0.46

(+)-borneol

mutant Y188T, pH 7.5, 30°C

0.0369

(-)-borneol

isoform BDH3, at pH 9.0 and 32°C

0.115

(-)-borneol

Pseudomonas sp. ATCC 17453

wild type enzyme, at pH 7.5 and 30°C

0.12

(-)-borneol

refolded isoform BDH1, at pH 7.0, temperature not specified in the publication

0.21

(-)-borneol

soluble isoform BDH1, at pH 7.0, temperature not specified in the publication

0.34

(-)-borneol

Pseudomonas sp. ATCC 17453

mutant enzyme S146A/Y188A, at pH 7.5 and 30°C

0.64

(-)-borneol

at pH 7.0 and 25°C

0.73

(-)-borneol

Pseudomonas sp. ATCC 17453

mutant enzyme S146A/Y188T, at pH 7.5 and 30°C

0.85

(-)-borneol

at pH 11.0 and 25°C

1.5

(-)-borneol

isoform BDH2, at pH 7.0, temperature not specified in the publication

0.006

NAD+

Pseudomonas sp. ATCC 17453

wild type enzyme, at pH 7.5 and 30°C

0.048

NAD+

isoform BDH2, at pH 8.0 and 20°C

0.06

NAD+

wild-type, pH 7.5, 30°C

0.07

NAD+

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0004 - 209

(+)-borneol

20 entries

0.0021 - 29.4

(-)-borneol

9 entries

0.0049 - 0.006

NAD+

0.0004

(+)-borneol

pH 8.0, 32°C

0.005

(+)-borneol

isoform BDH2, at pH 8.0 and 20°C

0.0054

(+)-borneol

isoform BDH3, at pH 8.5 and 30°C

0.0058

(+)-borneol

pH 9.0, 33°C, recombinant enzyme

0.5

(+)-borneol

refolded isoform BDH1, at pH 7.0, temperature not specified in the publication

0.5

(+)-borneol

refolded recombinant protein, pH 7, 25°C

0.75

(+)-borneol

recombinant enzyme, pH 8.5, 22°C

0.89

(+)-borneol

wild-type, pH 7.5, 30°C

0.89

(+)-borneol

Pseudomonas sp. ATCC 17453

at pH 7.5 and 30°C

1.51

(+)-borneol

Pseudomonas sp. ATCC 17453

mutant enzyme S146A/Y188A, at pH 7.5 and 30°C

1.51

(+)-borneol

mutant Y188A, pH 7.5, 30°C

3.32

(+)-borneol

Pseudomonas sp. ATCC 17453

mutant enzyme S146A/Y188T, at pH 7.5 and 30°C

3.32

(+)-borneol

mutant Y188T, pH 7.5, 30°C

(+)-borneol

soluble isoform BDH1, at pH 7.0, temperature not specified in the publication

(+)-borneol

soluble recombinant protein, pH 7, 25°C

27.8

(+)-borneol

isoform BDH2, at pH 7.0, temperature not specified in the publication

(+)-borneol

pH 7, 25°C

(+)-borneol

at pH 7.0 and 25°C

209

(+)-borneol

at pH 11.0 and 25°C