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Literature summary for 1.1.1.176 extracted from

  • Mythen, S.; Devendran, S.; Méndez-García, C.; Cann, I.; Ridlon, J.
    Targeted synthesis and characterization of a gene cluster encoding NAD(P)H-dependent 3alpha-, 3beta-, and 12alpha-hydroxysteroid dehydrogenases from Eggerthella CAG 298, a gut metagenomic sequence (2018), Appl. Environ. Microbiol., 84, e02475-17 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.176

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Escherichia coli Eggerthella sp. CAG:298

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0114
-
 12-oxo-deoxycholic acid coenzyme: NADPH, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298
0.1397
-
 deoxycholic acid coenzyme: NADP+, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26355
-
 calculation from sequence Eggerthella sp. CAG:298

Organism

Organism UniProt Comment Textmining
Eggerthella sp. CAG:298 R7AM69
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Eggerthella sp. CAG:298

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
12-oxo-cholic acid + NAD(P)H + H+ 4.1% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 cholic acid + NAD(P)+
-
 r
12-oxo-deoxycholic acid + NAD(P)H + H+
-
 Eggerthella sp. CAG:298 deoxycholic acid + NAD(P)+
-
 r
cholic acid + NAD(P)+ 55.7% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 12-oxocholic acid + NAD(P)H + H+
-
 r
deoxycholic acid + NAD(P)+ 51.9% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 12-oxo-deoxycholic acid + NAD(P)H + H+
-
 r
glycocholic acid + NAD(P)+ 60.3% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r
glycodeoxycholic acid + NAD(P)+ 55.9% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r
taurocholic acid + NAD(P)+ 53.8% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 2-[(3alpha,7alpha-dihydroxy-12,24-dioxo-5beta-cholan-24-yl)amino]ethane-1-sulfonic acid + NAD(P)H + H+
-
 r
taurodeoxychloic acid + NAD(P)+ 58.9% of the activity compared to the substrate 12-oxo-deoxycholic acid Eggerthella sp. CAG:298 ? + NAD(P)H + H+
-
 r

Subunits

Subunits Comment Organism
? x * 26355, calculation from sequence Eggerthella sp. CAG:298

Synonyms

Synonyms Comment Organism
12alpha-HSDH
-
 Eggerthella sp. CAG:298
CDD59475
-
 Eggerthella sp. CAG:298

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.63
-
 12-oxo-deoxycholic acid coenzyme: NADPH, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298
10
-
 deoxycholic acid coenzyme: NADP+, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 oxidative reaction and reductive reaction Eggerthella sp. CAG:298

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Eggerthella sp. CAG:298
NADPH
-
 Eggerthella sp. CAG:298

General Information

General Information Comment Organism
physiological function enzyme in an iso-bile acid pathway Eggerthella sp. CAG:298

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
71.6
-
 deoxycholic acid coenzyme: NADP+, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298
581.6
-
 12-oxo-deoxycholic acid coenzyme: NADPH, pH 7.0, temperature not specified in the publication Eggerthella sp. CAG:298