Literature summary for 1.1.1.159 extracted from

Zhang, X.; Fan, D.; Hua, X.; Zhang, T.
Large-scale production of ursodeoxycholic acid from chenodeoxycholic acid by engineering 7alpha- and 7beta-hydroxysteroid dehydrogenase (2019), Bioprocess Biosyst. Eng., 42, 1537-1545 .

Search for more literature for 1.1.1.159

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Clostridium sardiniense

Organism

Organism	UniProt	Comment	Textmining
Clostridium sardiniense	G9FRD7	-	-
Clostridium sardiniense DSM 599	G9FRD7	-	-

Purification (Commentary)

Purification (Comment)	Organism
GST-Sefinose column chromatography	Clostridium sardiniense

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
chenodeoxycholic acid + NADP+	in combination with a second reaction step with EC 1.1.1.201, 7beta-hydroxysteroid dehydrogenase, to ursodeoxycholic acid	Clostridium sardiniense	7-oxo-lithocholic acid + NADPH + H+	-	r
chenodeoxycholic acid + NADP+	in combination with a second reaction step with EC 1.1.1.201, 7beta-hydroxysteroid dehydrogenase, to ursodeoxycholic acid	Clostridium sardiniense DSM 599	7-oxo-lithocholic acid + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
?	x * 30000, SDS-PAGE	Clostridium sardiniense

Synonyms

Synonyms	Comment	Organism
7alpha-HSDH	-	Clostridium sardiniense

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Clostridium sardiniense

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Release 2023.1 (January 2023)