Cloned (Comment) | Organism |
---|---|
gene rdh, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) | Bradyrhizobium japonicum |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme in complex with both NAD+ and glucitol, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement and modelling | Bradyrhizobium japonicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc metalloenzyme | Bradyrhizobium japonicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26900 | - |
SDS-PAGE, subunit | Bradyrhizobium japonicum |
77700 | - |
native PAGE, recombinant enzyme | Bradyrhizobium japonicum |
83200 | - |
gel filtration, recombinant enzyme | Bradyrhizobium japonicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-iditol + NAD+ | Bradyrhizobium japonicum | i.e. D-sorbitol | D-sorbose + NADH + H+ | - |
r | |
D-iditol + NAD+ | Bradyrhizobium japonicum USDA110 | i.e. D-sorbitol | D-sorbose + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | Q89FN7 | i.e. Bradyrhizobium diazoefficiens | - |
Bradyrhizobium japonicum USDA110 | Q89FN7 | i.e. Bradyrhizobium diazoefficiens | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by heat treatment at 50°C and metal affinity chromatography to homogeneity | Bradyrhizobium japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucitol + NAD+ | - |
Bradyrhizobium japonicum | D-fructose + NADH + H+ | - |
r | |
D-glucitol + NAD+ | - |
Bradyrhizobium japonicum USDA110 | D-fructose + NADH + H+ | - |
r | |
D-iditol + NAD+ | i.e. D-sorbitol | Bradyrhizobium japonicum | D-sorbose + NADH + H+ | - |
r | |
D-iditol + NAD+ | i.e D-sorbitol | Bradyrhizobium japonicum | D-sorbose + NADH + H+ | - |
r | |
D-iditol + NAD+ | i.e. D-sorbitol | Bradyrhizobium japonicum USDA110 | D-sorbose + NADH + H+ | - |
r | |
D-iditol + NAD+ | i.e D-sorbitol | Bradyrhizobium japonicum USDA110 | D-sorbose + NADH + H+ | - |
r | |
L-glucitol + NAD+ | i.e. D-sorbitol, 90% conversion of L-glucitol to D-sorbose | Bradyrhizobium japonicum | D-sorbose + NADH + H+ | - |
r | |
L-glucitol + NAD+ | i.e. D-sorbitol, 90% conversion of L-glucitol to D-sorbose | Bradyrhizobium japonicum USDA110 | D-sorbose + NADH + H+ | - |
r | |
additional information | the substrate in BjSDH is stabilized via the side chain of Glu150, Ser140 and the carbonyl groups of Pro183 and Gly184 | Bradyrhizobium japonicum | ? | - |
? | |
additional information | the substrate in BjSDH is stabilized via the side chain of Glu150, Ser140 and the carbonyl groups of Pro183 and Gly184 | Bradyrhizobium japonicum USDA110 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | a continuous beta-sheet is formed between two monomers in the tetramer. The BjSDH structure folds in the Rossmann fold. The monomer of BjSDH is composed of a central parallel beta-sheet surrounded by three alpha-helices on each side. At the top of the beta-sheet a small helixturnhelix motif is located between the two final beta-strands betaF and betaG. Tetrameric quaternary organization, structure comparisons, overview | Bradyrhizobium japonicum |
tetramer | 4 * 26900, SDS-PAGE and crystal structure analysis | Bradyrhizobium japonicum |
Synonyms | Comment | Organism |
---|---|---|
BjSDH | - |
Bradyrhizobium japonicum |
D-sorbitol dehydrogenase | - |
Bradyrhizobium japonicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bradyrhizobium japonicum | |
NADH | dependent on | Bradyrhizobium japonicum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme BjSDH is part of the superfamily of Zn-independent short-chain dehydrogenases. The BjSDH structure folds in the Rossmann fold, which is typical for NADH-dependent enzymes and the SDR family | Bradyrhizobium japonicum |