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EC Tree
IUBMB Comments Also converts xylitol into L-xylulose and L-glucitol into L-fructose.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
BjSDH, D-sorbitol dehydrogenase, SDH, sorbitol dehydrogenase,
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BjSDH
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D-sorbitol dehydrogenase
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D-sorbitol dehydrogenase
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D-sorbitol dehydrogenase
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SDH
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sorbitol dehydrogenase
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sorbitol dehydrogenase
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D-iditol + NAD+ = D-sorbose + NADH + H+
Also converts xylitol into L-xylulose and L-glucitol into L-fructose
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D-iditol:NAD+ 2-oxidoreductase
Also converts xylitol into L-xylulose and L-glucitol into L-fructose.
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D-glucitol + NAD+
D-fructose + NADH + H+
D-iditol + NAD+
D-sorbose + NADH
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less stable enzyme, dulcitol-inducible, accounts for oxidation of certain fully hydroxylated polyols containing a D-threo-configuration adjacent to a primary alcohol
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?
D-iditol + NAD+
D-sorbose + NADH + H+
D-sorbitol + NAD+
D-fructose + NADH + H+
L-glucitol + NAD+
D-sorbose + NADH + H+
additional information
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D-glucitol + NAD+
D-fructose + NADH + H+
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r
D-glucitol + NAD+
D-fructose + NADH + H+
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r
D-iditol + NAD+
D-sorbose + NADH + H+
i.e. D-sorbitol
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r
D-iditol + NAD+
D-sorbose + NADH + H+
i.e D-sorbitol
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r
D-iditol + NAD+
D-sorbose + NADH + H+
i.e. D-sorbitol
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r
D-iditol + NAD+
D-sorbose + NADH + H+
i.e D-sorbitol
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r
D-sorbitol + NAD+
D-fructose + NADH + H+
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r
D-sorbitol + NAD+
D-fructose + NADH + H+
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r
L-glucitol + NAD+
D-sorbose + NADH + H+
i.e. D-sorbitol, 90% conversion of L-glucitol to D-sorbose
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r
L-glucitol + NAD+
D-sorbose + NADH + H+
i.e. D-sorbitol, 90% conversion of L-glucitol to D-sorbose
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r
additional information
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the substrate in BjSDH is stabilized via the side chain of Glu150, Ser140 and the carbonyl groups of Pro183 and Gly184
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additional information
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the substrate in BjSDH is stabilized via the side chain of Glu150, Ser140 and the carbonyl groups of Pro183 and Gly184
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D-iditol + NAD+
D-sorbose + NADH
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less stable enzyme, dulcitol-inducible, accounts for oxidation of certain fully hydroxylated polyols containing a D-threo-configuration adjacent to a primary alcohol
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?
D-iditol + NAD+
D-sorbose + NADH + H+
D-sorbitol + NAD+
D-fructose + NADH + H+
D-iditol + NAD+
D-sorbose + NADH + H+
i.e. D-sorbitol
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r
D-iditol + NAD+
D-sorbose + NADH + H+
i.e. D-sorbitol
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r
D-sorbitol + NAD+
D-fructose + NADH + H+
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r
D-sorbitol + NAD+
D-fructose + NADH + H+
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r
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additional information
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an NAD(H)-dependent enzyme
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NAD+
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NADH
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i.e. Bradyrhizobium diazoefficiens
UniProt
brenda
i.e. Bradyrhizobium diazoefficiens
UniProt
brenda
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brenda
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brenda
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brenda
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brenda
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evolution
the enzyme BjSDH is part of the superfamily of Zn-independent short-chain dehydrogenases. The BjSDH structure folds in the Rossmann fold, which is typical for NADH-dependent enzymes and the SDR family
evolution
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the enzyme BjSDH is part of the superfamily of Zn-independent short-chain dehydrogenases. The BjSDH structure folds in the Rossmann fold, which is typical for NADH-dependent enzymes and the SDR family
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Q0KAD3_CUPNH
Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337)
498
0
53155
TrEMBL
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A2R7N9_ASPNC
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
197
0
21904
TrEMBL
other Location (Reliability: 3 )
B6GYM5_PENRW
Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)
926
0
103596
TrEMBL
other Location (Reliability: 3 )
A0A380LNB4_9FIRM
379
0
43281
TrEMBL
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B6H4Q8_PENRW
Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255)
197
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21891
TrEMBL
other Location (Reliability: 2 )
A2QCY8_ASPNC
Aspergillus niger (strain CBS 513.88 / FGSC A1513)
944
0
104654
TrEMBL
other Location (Reliability: 4 )
A0A0F4YS17_TALEM
197
0
21882
TrEMBL
other Location (Reliability: 2 )
A0A060SWN7_BLAAD
193
0
21316
TrEMBL
other Location (Reliability: 3 )
Q89FN7_BRADU
Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)
242
0
26089
TrEMBL
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77700
native PAGE, recombinant enzyme
83200
gel filtration, recombinant enzyme
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tetramer
4 * 26900, SDS-PAGE and crystal structure analysis
tetramer
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4 * 26900, SDS-PAGE and crystal structure analysis
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additional information
a continuous beta-sheet is formed between two monomers in the tetramer. The BjSDH structure folds in the Rossmann fold. The monomer of BjSDH is composed of a central parallel beta-sheet surrounded by three alpha-helices on each side. At the top of the beta-sheet a small helix–turn–helix motif is located between the two final beta-strands betaF and betaG. Tetrameric quaternary organization, structure comparisons, overview
additional information
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a continuous beta-sheet is formed between two monomers in the tetramer. The BjSDH structure folds in the Rossmann fold. The monomer of BjSDH is composed of a central parallel beta-sheet surrounded by three alpha-helices on each side. At the top of the beta-sheet a small helix–turn–helix motif is located between the two final beta-strands betaF and betaG. Tetrameric quaternary organization, structure comparisons, overview
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purified recombinant His-tagged enzyme in complex with both NAD+ and glucitol, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement and modelling
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recombinant His-tagged enzyme from Escherichia coli strain BL21-Gold (DE3) by heat treatment at 50°C and metal affinity chromatography to homogeneity
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gene rdh, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21-Gold (DE3)
gene SDh, DNA and amino acid sequence determination and analysis, isolation, characterization and evaluation of the Pichia pastoris sorbitol dehydrogenase promoter for expression of heterologous proteins, recombinant expression of several different enzymes using the SDH promoter, overview
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Shaw, D.R.D.
Polyol dehydrogenases. 3. Galactitol dehydrogenase and D-iditol dehydrogenase
Biochem. J.
64
394-405
1956
Pseudomonas sp.
brenda
Fredslund, F.; Otten, H.; Gemperlein, S.; Poulsen, J.C.; Carius, Y.; Kohring, G.W.; Lo Leggio, L.
Structural characterization of the thermostable Bradyrhizobium japonicum D-sorbitol dehydrogenase
Acta Crystallogr. Sect. F
72
846-852
2016
Bradyrhizobium japonicum (Q89FN7), Bradyrhizobium japonicum USDA110 (Q89FN7)
brenda
Periyasamy, S.; Govindappa, N.; Sreenivas, S.; Sastry, K.
Isolation, characterization and evaluation of the Pichia pastoris sorbitol dehydrogenase promoter for expression of heterologous proteins
Protein Expr. Purif.
92
128-133
2013
Komagataella pastoris, Komagataella pastoris BICC 9450
brenda
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