Literature summary for 1.1.1.145 extracted from

Xu, D.; Wu, M.; Li, X.; Xia, M.; Liu, D.; Dai, Y.; Yu, Q.; Wu, B.; Wang, D.
Cloning, prokaryotic expression and function of the Bufo bufo gargarizans 3beta-hydroxysteroid dehydrogenase (3betaHSD) gene (2018), Int. J. Biol. Macromol., 120, 673-682 .

Search for more literature for 1.1.1.145

Cloned(Commentary)

Cloned (Comment)	Organism
cloned from the ear-side gland, expression in Escherichia coli	Bufo gargarizans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2313	-	pregnenolone	pH 7.8, 30°C	Bufo gargarizans
0.2419	-	dehydroepiandrosterone	pH 7.8, 30°C	Bufo gargarizans

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42800	-	calculated from sequence	Bufo gargarizans

Organism

Organism	UniProt	Comment	Textmining
Bufo gargarizans	A0A345GNZ1	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Bufo gargarizans

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	expression of 3beta-hydroxysteroid dehydrogenase (3betaHSD) in ear-side gland is nearly 20times higher than that in liver	Bufo gargarizans	-
parotid gland	expression of 3beta-hydroxysteroid dehydrogenase (3betaHSD) in ear-side gland is nearly 20times higher than that in liver	Bufo gargarizans	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dehydroepiandrosterone + NAD+	when the coenzyme is NADP+ the yield decreases significantly	Bufo gargarizans	androstenedione + NADH + H+	-	?
pregnenolone + NAD+	NAD+ is more suitable coenzyme than NADP+	Bufo gargarizans	progesterone + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
3beta-hydroxysteroid dehydrogenase	-	Bufo gargarizans
3betaHSD	-	Bufo gargarizans

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	recombinant enzyme	Bufo gargarizans

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	50	30°C: about 30% of maximal activity, 30°C: about 35% of maximal activity	Bufo gargarizans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.64	-	dehydroepiandrosterone	pH 7.8, 30°C	Bufo gargarizans
2.8	-	pregnenolone	pH 7.8, 30°C	Bufo gargarizans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	recombinant enzyme	Bufo gargarizans

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	10	pH 6.5: about 55% of maximal activity, pH 10.0: about 45% of maximal activity	Bufo gargarizans

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	optimum coenzyme concentration is 1.5 mmol/l	Bufo gargarizans

pI Value

Organism	Comment	pI Value Maximum	pI Value
Bufo gargarizans	calculated from sequence	-	8.68

General Information

General Information	Comment	Organism
metabolism	the enzyme catalyzes an essential step in the biosynthesis of steroid such as progesterone, estrogens and androgens in steroidogenic tissues. It is probably an important enzyme involved in the biosynthesis of bufadienolides	Bufo gargarizans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.65	-	dehydroepiandrosterone	pH 7.8, 30°C	Bufo gargarizans
12.12	-	pregnenolone	pH 7.8, 30°C	Bufo gargarizans

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)