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Literature summary for 1.1.1.140 extracted from

  • Salomone-Stagni, M.; Bartho, J.D.; Kalita, E.; Rejzek, M.; Field, R.A.; Bellini, D.; Walsh, M.A.; Benini, S.
    Structural and functional analysis of Erwinia amylovora SrlD. The first crystal structure of a sorbitol-6-phosphate 2-dehydrogenase (2018), J. Struct. Biol., 203, 109-119 .
    View publication on PubMed
Search for more literature for 1.1.1.140

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3)Star-pLys cells Erwinia amylovora

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor-diffusion at 0°C, the crystal structure is determined at 1.84 A resolution Erwinia amylovora

Inhibitors

Inhibitors Comment Organism Structure
additional information conversion of D-sorbitol 6-phosphate into D-fructose 6-phosphate is not inhibited by D-mannose 6-phosphate at inhibitor concentrations as high as 10 mM. Conversion of D-fructose 6-phosphate into D-sorbitol 6-phosphate is not observed with concentrations of D-mannose 6-phosphate as high as 5 mM Erwinia amylovora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 NADH pH 9.0, 22°C Erwinia amylovora
1.6
-
 NAD+ pH 9.0, 22°C Erwinia amylovora
2
-
 D-fructose 6-phosphate pH 9.0, 22°C Erwinia amylovora
9
-
 D-sorbitol 6-phosphate pH 9.0, 22°C Erwinia amylovora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-sorbitol 6-phosphate + NAD+ Erwinia amylovora in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) D-fructose 6-phosphate + NADH + H+
-
 ?
D-sorbitol 6-phosphate + NAD+ Erwinia amylovora CFBP1430 in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) D-fructose 6-phosphate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Erwinia amylovora D4I194
-
-
Erwinia amylovora CFBP1430 D4I194
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Erwinia amylovora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 6-phosphate + NADH + H+ the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate Erwinia amylovora D-sorbitol 6-phosphate + NAD+
-
 r
D-fructose 6-phosphate + NADH + H+ the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate Erwinia amylovora CFBP1430 D-sorbitol 6-phosphate + NAD+
-
 r
D-sorbitol 6-phosphate + NAD+ in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) Erwinia amylovora D-fructose 6-phosphate + NADH + H+
-
 ?
D-sorbitol 6-phosphate + NAD+ the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate Erwinia amylovora D-fructose 6-phosphate + NADH + H+
-
 r
D-sorbitol 6-phosphate + NAD+ in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) Erwinia amylovora CFBP1430 D-fructose 6-phosphate + NADH + H+
-
 ?
D-sorbitol 6-phosphate + NAD+ the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate Erwinia amylovora CFBP1430 D-fructose 6-phosphate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
SrlD
-
 Erwinia amylovora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.56
-
 D-fructose 6-phosphate pH 9.0, 22°C Erwinia amylovora
5.6
-
 D-sorbitol 6-phosphate pH 9.0, 22°C Erwinia amylovora

Cofactor

Cofactor Comment Organism Structure
NAD+ Asp61 drives the specificity for NAD(H) over NADP(H) Erwinia amylovora
NADH Asp61 drives the specificity for NAD(H) over NADP(H) Erwinia amylovora

General Information

General Information Comment Organism
metabolism in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince) Erwinia amylovora

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.28
-
 D-fructose 6-phosphate pH 9.0, 22°C Erwinia amylovora
0.62
-
 D-sorbitol 6-phosphate pH 9.0, 22°C Erwinia amylovora