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Information on EC 1.1.1.140 - sorbitol-6-phosphate 2-dehydrogenase
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1.1.1.140 sorbitol-6-phosphate 2-dehydrogenaseSpecify your search results
Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
s6pdh, sorbitol-6-phosphate dehydrogenase, d-glucitol-6-phosphate dehydrogenase, glucitol-6-phosphate dehydrogenase, d-sorbitol-6-phosphate dehydrogenase, d-sorbitol-6-phosphate 2-dehydrogenase, mds6pdh, sorbitol-6-phosphate 2-dehydrogenase, stl6pdh, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D-glucitol-6-phosphate dehydrogenase
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D-sorbitol-6-phosphate 2-dehydrogenase
D-sorbitol-6-phosphate dehydrogenase
dehydrogenase, D-sorbitol 6-phosphate
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Glucitol-6-phosphate dehydrogenase
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HmSorD
ketosephosphate reductase
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S6PDH
sorbitol-6-P-dehydrogenase
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sorbitol-6-phosphate dehydrogenase
SrlD
additional information
D-sorbitol-6-phosphate dehydrogenase
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additional information
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similar enzyme also named sorbitol-6-phosphate dehydrogenase from Eriobotrya japonica catalyzes following reaction: sorbitol-6-phosphate + NADP+ = glucose-6-phosphate + NADPH
additional information
-
similar enzyme also named sorbitol-6-phosphate dehydrogenase from Eriobotrya japonica catalyzes following reaction: sorbitol-6-phosphate + NADP+ = glucose-6-phosphate + NADPH
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-sorbitol 6-phosphate + NAD+ = D-fructose 6-phosphate + NADH + H+
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
D-sorbitol-6-phosphate:NAD+ 2-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY
37250-69-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-dulcitol 6-phosphate + NAD+
?
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at 3% of reaction reaction with sorbitol 6-phosphate
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?
mannitol 1-phosphate + NAD+
?
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at 1-5% of reaction with sorbitol 6-phosphate
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?
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate
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r
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate
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r
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
AAV45891.1
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?
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
AAV45891.1
the recombinant enzyme (HmS6PDH) has no activity to glucose or sorbitol
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?
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
AAV45891.1
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?
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
AAV45891.1
the recombinant enzyme (HmS6PDH) has no activity to glucose or sorbitol
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?
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
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sorbitol is not detected when Stl6PDH is not overexpressed
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?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for NAD+
high specificity for D-fructose 6-phosphate
r
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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equilibrium far on the side of sorbitol 6-phosphate formation
high specificity for D-fructose 6-phosphate
r
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for D-sorbitol 6-phosphate
high specificity for D-fructose 6-phosphate
r
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince)
-
-
?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate
-
-
r
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince)
-
-
?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
the enzyme is much faster at oxidizing D-sorbitol 6-phosphate than in reducing D-fructose 6-phosphate, however, equilibrium analysis reveals that only part of the D-sorbitol 6-phosphate present in the in vitro environment is converted into D-fructose 6-phosphate
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r
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for NAD+
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?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for D-sorbitol 6-phosphate
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?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for NAD+
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?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for D-sorbitol 6-phosphate
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-
?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for NAD+
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r
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for D-sorbitol 6-phosphate
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r
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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high specificity for D-sorbitol 6-phosphate
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r
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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hexitol catabolic enzyme
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?
D-sorbitol 6-phosphate + NADP+
D-fructose 6-phosphate + NADPH + H+
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key enzyme in the regulation of the sorbitol/sucrose ratio in apple leaves
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?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-sorbitol 6-phosphate + NADP+
D-fructose 6-phosphate + NADPH + H+
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key enzyme in the regulation of the sorbitol/sucrose ratio in apple leaves
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?
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
AAV45891.1
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?
D-fructose 6-phosphate + NADH + H+
D-sorbitol 6-phosphate + NAD+
AAV45891.1
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-
-
?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince)
-
-
?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince)
-
-
?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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-
?
D-sorbitol 6-phosphate + NAD+
D-fructose 6-phosphate + NADH + H+
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hexitol catabolic enzyme
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
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inhibited by alteration of the 6-amino position of the adenine moiety of NAD+
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
NaCl
AAV45891.1
activity is increased by nearly 120% when NaCl concentration is 2 M. Transgenic Arabidopsis thaliana ectopic expressing the enzyme (HmS6PDH) accumulates more sorbitol under salt stress
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3-(5-((E)-[3,5-bis(methoxycarbonyl)phenyl]diazenyl)-2-hydroxyphenyl)propanoic acid
enzyme inhibition is accompanied by a decrease in total prostaglandin F2alpha
5-phospho-D-arabinonohydroxamic acid
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substrate analogue inhibitor, IC50: 0.040 mM
disodium 4-(5-[(Z)-(4-oxido-2-oxo-1,3-thiazol-5(2H)-ylidene)methyl]furan-2-yl)benzoate
reduces deactivated prostaglandin F2alpha metabolite production in outer root sheath keratinocyte supernatant
ethyl 3-(5-[(2,4-dioxo-1,3-thiazolidin-5-yl)methyl]furan-2-yl)benzoate
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additional information
conversion of D-sorbitol 6-phosphate into D-fructose 6-phosphate is not inhibited by D-mannose 6-phosphate at inhibitor concentrations as high as 10 mM. Conversion of D-fructose 6-phosphate into D-sorbitol 6-phosphate is not observed with concentrations of D-mannose 6-phosphate as high as 5 mM
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additional information
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conversion of D-sorbitol 6-phosphate into D-fructose 6-phosphate is not inhibited by D-mannose 6-phosphate at inhibitor concentrations as high as 10 mM. Conversion of D-fructose 6-phosphate into D-sorbitol 6-phosphate is not observed with concentrations of D-mannose 6-phosphate as high as 5 mM
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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gut genes are induced by sorbitol. GutR codes for a transcriptional activator of gut genes
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
overview: various buffers, various substrate concentrations
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00015
3-(5-((E)-[3,5-bis(methoxycarbonyl)phenyl]diazenyl)-2-hydroxyphenyl)propanoic acid
Pyrus pyrifolia
pH 7.5, 37°C
0.0051
5-([5-(3-chlorophenyl)furan-2-yl]methyl)-1,3-thiazolidine-2,4-dione
Pyrus pyrifolia
pH 7.5, 37°C
0.048
5-phospho-D-arabinonate
Escherichia coli
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substrate analogue inhibitor, IC50: 0.048 mM
0.04
5-phospho-D-arabinonohydroxamic acid
Escherichia coli
-
substrate analogue inhibitor, IC50: 0.040 mM
0.0003
disodium 4-(5-[(Z)-(4-oxido-2-oxo-1,3-thiazol-5(2H)-ylidene)methyl]furan-2-yl)benzoate
Pyrus pyrifolia
pH 7.5, 37°C
0.0046
ethyl 3-(5-[(2,4-dioxo-1,3-thiazolidin-5-yl)methyl]furan-2-yl)benzoate
Pyrus pyrifolia
pH 7.5, 37°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
activities of different recombinant strains
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
8.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2.5 - 5.5
AAV45891.1
pH 2.5: about 40% of maximal activity, pH 5.5: about 45% of maximal activity
5.7 - 7.5
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pH 5.7: about 50% of activity maximum, pH 7.5: about 35% of activity maximum
additional information
-
in Tris buffer 32times greater activity than in NaHCO3 buffer
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
50 - 70
AAV45891.1
50°C: about 50% of maximal activity, 70°C: about 30% of maximal activity
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
similar enzyme also named sorbitol-6-phosphate dehydrogenase catalyzes following reaction: sorbitol-6-phosphate + NADP+ = glucose 6-phosphate + NADPH
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brenda
with a temperature-sensitive enzyme, strains grown at 40°C with much lower activity than them grown at 30°C
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brenda
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
expression of mRNA, no detection of protein or its catalytic activity
brenda
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similar enzyme also named sorbitol-6-phosphate dehydrogenase from Eriobotrya japonica
brenda
expression of mRNA, no detection of protein or its catalytic activity
brenda
high level of expression at 30 days after full blooming, expression decreases at 38 days after full blooming and then is maintained at a constant level
brenda
-
similar enzyme also named sorbitol-6-phosphate dehydrogenase from Eriobotrya japonica
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
metabolism
in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince)
metabolism
-
in the plant pathogen Erwinia amylovora the enzyme (S6PDH, SrlD) is used by the bacterium to utilize sorbitol, which is used for carbohydrate transport in the host plants belonging to the Amygdaloideae subfamily (e.g., apple, pear, and quince)
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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