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Literature summary for 1.1.1.14 extracted from

  • Shibata, T.; Ichikawa, C.; Matsuura, M.; Takata, Y.; Noguchi, Y.; Saito, Y.; Yamashita, M.
    Cloning of a gene for D-sorbitol dehydrogenase from Gluconobacter oxydans G624 and expression of the gene in Pseudomonas putida IFO3738 (2000), J. Biosci. Bioeng., 89, 463-468.
    View publication on PubMed
Search for more literature for 1.1.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pseudomonas putida strain IFO3738 fused to 6 x His-tag Gluconobacter oxydans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
132
-
 D-sorbitol in the presence of NADP+ (pH 10.1) Gluconobacter oxydans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53640
-
 calculated from amino acid sequence Gluconobacter oxydans

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans Q9KWR5
-
-
Gluconobacter oxydans G624 (FERM BP-4415) Q9KWR5
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt-based resin affinity chromatography Gluconobacter oxydans

Storage Stability

Storage Stability Organism
-20°C, purified recombinant enzyme, 3d, almost complete loss of activity Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol + NAD(P)+
-
 Gluconobacter oxydans D-xylulose + NAD(P)H + H+
-
 ?
D-arabitol + NAD(P)+
-
 Gluconobacter oxydans G624 (FERM BP-4415) D-xylulose + NAD(P)H + H+
-
 ?
D-mannitol + NAD(P)+
-
 Gluconobacter oxydans D-fructose + NAD(P)H + H+
-
 ?
D-mannitol + NAD(P)+
-
 Gluconobacter oxydans G624 (FERM BP-4415) D-fructose + NAD(P)H + H+
-
 ?
D-sorbitol + NAD(P)+
-
 Gluconobacter oxydans L-sorbose + NAD(P)H + H+
-
 ?
D-sorbitol + NAD(P)+
-
 Gluconobacter oxydans G624 (FERM BP-4415) L-sorbose + NAD(P)H + H+
-
 ?
additional information no activity against D-xylitol, D-ribitol, D-inositol or glycerol Gluconobacter oxydans ?
-
 ?
additional information no activity against D-xylitol, D-ribitol, D-inositol or glycerol Gluconobacter oxydans G624 (FERM BP-4415) ?
-
 ?

Synonyms

Synonyms Comment Organism
D-sorbitol dehydrogenase
-
 Gluconobacter oxydans
SLDH
-
 Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 native enzyme Gluconobacter oxydans
10.1
-
 recombinant enzyme Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Gluconobacter oxydans
NADP+ exhibits 15 times higher activity in the presence of NADP+ than that of NAD+ Gluconobacter oxydans