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Literature summary for 1.1.1.14 extracted from
- Purification and properties of membrane-bound D-sorbitol dehydrogenase from Gluconobacter suboxydans IFO 3255 (2002), Biosci. Biotechnol. Biochem., 66, 57-64.
General Stability
| General Stability | Organism |
|---|---|
| Triton X-100, 1%, and D-sorbitol stabilize the enzyme during solubilization and purification, while n-octyl-beta-D-glucopyranoside does not | Gluconobacter oxydans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | complete inhibition at 0.91 mM | Gluconobacter oxydans |  |
| Fe2+ | complete inhibition at 0.91 mM | Gluconobacter oxydans | |
| KCl | 0.1 M, inactivation | Gluconobacter oxydans | |
| Mg2+ | slightly inhibitory | Gluconobacter oxydans | |
| Mn2+ | slightly inhibitory | Gluconobacter oxydans | |
| monoiodoacetate | 75% inhibition at 1.79 mM | Gluconobacter oxydans | |
| quinine x HCl | slightly inhibitory, 25% inhibition at 1.79 mM | Gluconobacter oxydans | |
| Zn2+ | - |
Gluconobacter oxydans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18 | - |
D-sorbitol | pH 6.0, 25°C, with 2,6-dichlorophenolindophenol as electron acceptor | Gluconobacter oxydans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | bound | Gluconobacter oxydans | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | slightly activating | Gluconobacter oxydans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 800000 | - |
gel filtration | Gluconobacter oxydans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
| Gluconobacter oxydans IFO 3255 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by solubilization with Triton X-100, DEAE-cellulose and -Sepharose, hydroxylapatite, and size exclusion chromatography, efficiency of different solubilization methods, overview | Gluconobacter oxydans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 45.3 | - |
purified enzyme | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-adonitol + NAD+ | - |
Gluconobacter oxydans | D-ribulose + NADH | - |
? | |
| D-arabitol + NAD+ | best substrate | Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
? | |
| D-arabitol + NAD+ | best substrate | Gluconobacter oxydans IFO 3255 | D-xylulose + NADH + H+ | - |
? | |
| D-gluconate + NAD+ | low activity | Gluconobacter oxydans | ? | - |
? | |
| D-mannitol + NAD+ | - |
Gluconobacter oxydans | D-fructose + NADH + H+ | - |
? | |
| D-mannitol + NAD+ | - |
Gluconobacter oxydans IFO 3255 | D-fructose + NADH + H+ | - |
? | |
| D-sorbitol + 2,6-dichlorophenolindophenol | electron acceptor: i.e. DCIP | Gluconobacter oxydans | L-sorbose + ? | - |
? | |
| D-sorbitol + NAD+ | - |
Gluconobacter oxydans | L-sorbose + NADH + H+ | - |
? | |
| D-sorbitol + NAD+ | - |
Gluconobacter oxydans IFO 3255 | L-sorbose + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Gluconobacter oxydans | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Gluconobacter oxydans IFO 3255 | dihydroxyacetone + NADH + H+ | - |
? | |
| meso-erythritol + NAD+ | high activity | Gluconobacter oxydans | erythrulose + NADH | - |
? | |
| additional information | no activity with D-glucose, D-fructose, L-sorbose, methanol, ethanol, and sucrose | Gluconobacter oxydans | ? | - |
? | |
| additional information | no activity with D-glucose, D-fructose, L-sorbose, methanol, ethanol, and sucrose | Gluconobacter oxydans IFO 3255 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| decamer | 10 x 80000, SDS-PAGE | Gluconobacter oxydans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-sorbitol dehydrogenase | - |
Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Gluconobacter oxydans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | maximal activity at 30°C, 40% activity at 45°C, 30% activity at 50°C, no activity at 60°C | Gluconobacter oxydans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
stable up to | Gluconobacter oxydans |
| 40 | - |
loss of 20% activity | Gluconobacter oxydans |
| 50 | - |
loss of 70% activity | Gluconobacter oxydans |
| 60 | - |
loss of 90% activity | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Gluconobacter oxydans | |
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