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Literature summary for 1.1.1.14 extracted from

  • Hoshino, T.; Sugisawa, T.; Shinjoh, M.; Tomiyama, N.; Miyazaki, T.
    Membrane-bound D-sorbitol dehydrogenase of Gluconobacter suboxydans IFO 3255 - enzymatic and genetic characterization (2003), Biochim. Biophys. Acta, 1647, 278-288.
    View publication on PubMed
Search for more literature for 1.1.1.14

Activating Compound

Activating Compound Comment Organism Structure
additional information enzyme needs product, i.e. SldB, of upstream gene sldB, encoding a hydrophobic polypeptide, for activity, the polypeptide might be a chaperone-like component that signals processing and folding of the enzyme to give the active enzyme form Gluconobacter oxydans

Application

Application Comment Organism
synthesis L-sorbose is an important intermediate in the industrial vitamin C production process Gluconobacter oxydans

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, co-expression with gene sldB in Escherichia coli strain JM109, sldB and PQQ are required for recombinant activity in Escherichia coli, co-expression of sldB is also required for enzyme activity in vivo Gluconobacter oxydans

Protein Variants

Protein Variants Comment Organism
additional information construction of gene disruption mutants of genes sldA and sldB, the mutants are inactive, the sldB mutation leads to a higher enzyme expression and accumulation of unprocessed SldA, co-expression of sldB is required for enzyme activity in vivo Gluconobacter oxydans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound, 2 isozymes Gluconobacter oxydans 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 1 * 80000, SDS-PAGE Gluconobacter oxydans
80000
-
 3 * 80000 Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-sorbitol + NAD+ Gluconobacter oxydans
-
 L-sorbose + NADH + H+
-
 ?
D-sorbitol + NAD+ Gluconobacter oxydans IFO 3255
-
 L-sorbose + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
 precursor; gene sldA
-
Gluconobacter oxydans IFO 3255
-
 precursor; gene sldA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol + NAD+
-
 Gluconobacter oxydans D-fructose + NADH + H+
-
 ?
D-mannitol + NAD+
-
 Gluconobacter oxydans IFO 3255 D-fructose + NADH + H+
-
 ?
D-sorbitol + NAD+
-
 Gluconobacter oxydans L-sorbose + NADH + H+
-
 ?
D-sorbitol + NAD+
-
 Gluconobacter oxydans IFO 3255 L-sorbose + NADH + H+
-
 ?
glycerol + NAD+
-
 Gluconobacter oxydans dihydroxyacetone + NADH + H+
-
 ?
glycerol + NAD+
-
 Gluconobacter oxydans IFO 3255 dihydroxyacetone + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 80000, SDS-PAGE Gluconobacter oxydans
trimer 3 * 80000 Gluconobacter oxydans

Synonyms

Synonyms Comment Organism
D-sorbitol dehydrogenase
-
 Gluconobacter oxydans
SLDH
-
 Gluconobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 broad pH spectrum in vivo Gluconobacter oxydans