Literature summary for 1.1.1.14 extracted from

Takayuki, U.
Purification and properties of NAD+-dependent sorbitol dehydrogenase from Bacillus fructosus (1999), Biosci. Biotechnol. Biochem., 63, 573-574.

Search for more literature for 1.1.1.14

Crystallization (Commentary)

Crystallization (Comment)	Organism
-	Priestia megaterium

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	-	Priestia megaterium
Cu2+	-	Priestia megaterium
Hg2+	-	Priestia megaterium
p-chloromercuribenzoate	-	Priestia megaterium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.22	-	NAD+	-	Priestia megaterium
11	-	D-sorbitol	-	Priestia megaterium

Organism

Organism	UniProt	Comment	Textmining
Priestia megaterium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Priestia megaterium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.7	-	-	Priestia megaterium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-sorbitol + NAD+	-	Priestia megaterium	D-fructose + NADH + H+	-	r

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	30 min, stable	Priestia megaterium
50	-	5% loss of activity after 30 min	Priestia megaterium

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
5	9	30 min at 30°C	Priestia megaterium

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Priestia megaterium
NADH	-	Priestia megaterium

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Release 2023.1 (January 2023)