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Information on EC 6.6.1.1 - magnesium chelatase and Organism(s) Chlamydomonas reinhardtii and UniProt Accession Q94FT3

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EC Tree
     6 Ligases
         6.6 Forming nitrogen—metal bonds
             6.6.1 Forming coordination complexes
                6.6.1.1 magnesium chelatase
IUBMB Comments
This is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway.
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This record set is specific for:
Chlamydomonas reinhardtii
UNIPROT: Q94FT3
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Word Map
The taxonomic range for the selected organisms is: Chlamydomonas reinhardtii
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
magnesium chelatase, mg-chelatase, mg chelatase, chli1, xantha-f, abar/chlh, magnesium-chelatase, mg-chelatase h, chelatase h subunit, chli protein, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
magnesium-chelatase
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magnesium-protoporphyrin chelatase
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magnesium-protoporphyrin IX chelatase
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Mg-chelatase
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Mg-protoporphyrin IX magnesio-lyase
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protoporphyrin IX magnesium-chelatase
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protoporphyrin IX Mg-chelatase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ligation
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SYSTEMATIC NAME
IUBMB Comments
Mg-protoporphyrin IX magnesium-lyase
This is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway.
CAS REGISTRY NUMBER
COMMENTARY hide
9074-88-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + protoporphyrin IX + Mg2+ + H2O
ADP + phosphate + Mg-protoporphyrin IX + 2 H+
show the reaction diagram
additional information
?
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the pattern of changes in RNA transcript levels of the magnesium chelatase genes, chlH, chlD and chlI of Chlamydomonas reinhardtii grown under synchronous culture conditions in light/dark cycles are similar. Light is involved in regulation
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + protoporphyrin IX + Mg2+ + H2O
ADP + phosphate + Mg-protoporphyrin IX + 2 H+
show the reaction diagram
additional information
?
-
-
the pattern of changes in RNA transcript levels of the magnesium chelatase genes, chlH, chlD and chlI of Chlamydomonas reinhardtii grown under synchronous culture conditions in light/dark cycles are similar. Light is involved in regulation
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
in wild-type cells, a shift from dark to light results in a transient reduction in heme levels, while the levels of Mg-protoporphyrin IX, its methyl ester, and protoporphyrin IX increase. Hemin feeding to cultures in the dark activates HSP70A
Uniprot
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CHLI_CHLRE
417
0
45393
Swiss-Prot
Chloroplast (Reliability: 4)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 150000, subunit ChlH, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography and Superdex 200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lake, V.; Willows, R.D.
Rapid extraction of RNA and analysis of transcript levels in Chlamydomonas reinhardtii using real-time RT-PCR: Magnesium chelatase chlH, chlD and chlI gene expression
Photosynth. Res.
77
69-76
2003
Chlamydomonas reinhardtii
Manually annotated by BRENDA team
von Gromoff, E.D.; Alawady, A.; Meinecke, L.; Grimm, B.; Beck, C.F.
Heme, a plastid-derived regulator of nuclear gene expression in Chlamydomonas
Plant Cell
20
552-567
2008
Chlamydomonas reinhardtii (Q94FT3), Chlamydomonas reinhardtii
Manually annotated by BRENDA team
Mueller, A.H.; Sawicki, A.; Zhou, S.; Tabrizi, S.T.; Luo, M.; Hansson, M.; Willows, R.D.
Inducing the oxidative stress response in Escherichia coli improves the quality of a recombinant protein magnesium chelatase ChlH
Protein Expr. Purif.
101
61-67
2014
Chlamydomonas reinhardtii (A8I7P5), Chlamydomonas reinhardtii, Chlamydomonas reinhardtii CC124 (A8I7P5), Hordeum vulgare, Oryza sativa
Manually annotated by BRENDA team
Chekunova, E.; Yaronskaya, E.; Yartseva, N.; Averina, N.
New factors regulating magnesium chelatase in the green alga Chlamydomonas reinhardtii
Russ. J. Plant Physiol.
61
169-177
2014
Chlamydomonas reinhardtii, Chlamydomonas reinhardtii CC124
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Manually annotated by BRENDA team