Literature summary for 6.6.1.1 extracted from

Mueller, A.H.; Sawicki, A.; Zhou, S.; Tabrizi, S.T.; Luo, M.; Hansson, M.; Willows, R.D.
Inducing the oxidative stress response in Escherichia coli improves the quality of a recombinant protein magnesium chelatase ChlH (2014), Protein Expr. Purif., 101, 61-67 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
GUN4 protein	-	Hordeum vulgare
GUN4 protein	-	Oryza sativa
GUN4 protein	-	Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Hordeum vulgare
expressed in Escherichia coli BL21(DE3) cells	Oryza sativa
expressed in Escherichia coli BL21(DE3) cells	Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + protoporphyrin IX + Mg2+ + H2O	Hordeum vulgare	-	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?
ATP + protoporphyrin IX + Mg2+ + H2O	Oryza sativa	-	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?
ATP + protoporphyrin IX + Mg2+ + H2O	Chlamydomonas reinhardtii	-	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?
ATP + protoporphyrin IX + Mg2+ + H2O	Chlamydomonas reinhardtii CC124	-	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	A8I7P5	-	-
Chlamydomonas reinhardtii CC124	A8I7P5	-	-
Hordeum vulgare	-	cultivar Svalöfs Bonus	-
Oryza sativa	-	subspecies Oryza sativa japonica, cultivar Nipponbare	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography and Superdex 200 gel filtration	Hordeum vulgare
HisTrap column chromatography and Superdex 200 gel filtration	Oryza sativa
HisTrap column chromatography and Superdex 200 gel filtration	Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + protoporphyrin IX + Mg2+ + H2O	-	Hordeum vulgare	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?
ATP + protoporphyrin IX + Mg2+ + H2O	-	Oryza sativa	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?
ATP + protoporphyrin IX + Mg2+ + H2O	-	Chlamydomonas reinhardtii	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?
ATP + protoporphyrin IX + Mg2+ + H2O	-	Chlamydomonas reinhardtii CC124	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?

Subunits

Subunits	Comment	Organism
?	x * 150000, subunit ChlH, SDS-PAGE	Hordeum vulgare
?	x * 150000, subunit ChlH, SDS-PAGE	Oryza sativa
?	x * 150000, subunit ChlH, SDS-PAGE	Chlamydomonas reinhardtii

Synonyms

Synonyms	Comment	Organism
ChlH	subunit	Hordeum vulgare
ChlH	subunit	Oryza sativa
ChlH	subunit	Chlamydomonas reinhardtii
Mg chelatase	-	Hordeum vulgare
Mg chelatase	-	Oryza sativa
Mg chelatase	-	Chlamydomonas reinhardtii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Hordeum vulgare
ATP	-	Oryza sativa
ATP	-	Chlamydomonas reinhardtii

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Release 2023.1 (January 2023)