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The taxonomic range for the selected organisms is: Pinus taeda
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
4-coumarate:coa ligase, 4-coumarate:coenzyme a ligase, 4-coumarate-coa ligase, 4-coumarate coa ligase, 4-coumarate coenzyme a ligase, at4cl1, 4-coumaroyl-coa ligase, os4cl, pl4cl1, pl4cl2,
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4-Coumarate:coenzyme A ligase
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4-coumaroyl-CoA synthase
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-
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4-Coumaryl-CoA synthetase
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-
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Caffeolyl coenzyme A synthetase
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-
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Feruloyl CoA ligase
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Feruloyl coenzyme A synthetase
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-
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Hydroxy-cinnamate:CoA ligase
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Hydroxycinnamate:CoA ligase
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Hydroxycinnamoyl CoA synthetase
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p-Coumaroyl CoA ligase
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p-Coumaryl coenzyme A synthetase
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p-Coumaryl-CoA ligase
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p-Coumaryl-CoA synthetase
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p-Hydroxycinnamic acid:CoA ligase
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p-Hydroxycinnamoyl coenzyme A synthetase
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Sinapoyl coenzyme A snthetase
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Synthetase, p-coumaroyl coenzyme A
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Acid-thiol ligation
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-, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -
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4-coumarate:CoA ligase (AMP-forming)
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ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
ATP + caffeate + CoA
AMP + diphosphate + caffeoyl-CoA
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-
-
?
ATP + cinnamate + CoA
AMP + diphosphate + cinnamoyl-CoA
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-
-
?
ATP + ferulate + CoA
AMP + diphosphate + feruloyl-CoA
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-
-
?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
ATP + caffeate + CoA
AMP + diphosphate + caffeoyl-CoA
ATP + cinnamate + CoA
AMP + diphosphate + cinnamoyl-CoA
ATP + ferulate + CoA
AMP + diphosphate + feruloyl-CoA
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
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-
-
?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
preferred substrate
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-
?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
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?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
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-
?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
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-
-
?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
preferred substrate
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-
?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
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plant phenylpropanoid metabolism
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?
ATP + caffeate + CoA
AMP + diphosphate + caffeoyl-CoA
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-
?
ATP + caffeate + CoA
AMP + diphosphate + caffeoyl-CoA
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?
ATP + caffeate + CoA
AMP + diphosphate + caffeoyl-CoA
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same activity as with 4-coumarate
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?
ATP + cinnamate + CoA
AMP + diphosphate + cinnamoyl-CoA
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-
?
ATP + cinnamate + CoA
AMP + diphosphate + cinnamoyl-CoA
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-
-
?
ATP + ferulate + CoA
AMP + diphosphate + feruloyl-CoA
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-
?
ATP + ferulate + CoA
AMP + diphosphate + feruloyl-CoA
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?
ATP + ferulate + CoA
AMP + diphosphate + feruloyl-CoA
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same activity as with 4-coumarate
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?
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ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
preferred substrate
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-
?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
preferred substrate
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-
?
ATP + 4-coumarate + CoA
AMP + diphosphate + 4-coumaroyl-CoA
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plant phenylpropanoid metabolism
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?
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0.0302
4-coumarate
isoform 4CL1, pH and temperature not specified in the publication
0.0296
caffeate
isoform 4CL1, pH and temperature not specified in the publication
1.0803
cinnamate
isoform 4CL1, pH and temperature not specified in the publication
0.0522
ferulate
isoform 4CL1, pH and temperature not specified in the publication
0.01097
4-coumarate
isoform 4CL3, pH and temperature not specified in the publication
0.0423
caffeate
isoform 4CL3, pH and temperature not specified in the publication
0.8875
cinnamate
isoform 4CL3, pH and temperature not specified in the publication
0.0849
ferulate
isoform 4CL3, pH and temperature not specified in the publication
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4.15
4-coumarate
isoform 4CL1, pH and temperature not specified in the publication
1.6
caffeate
isoform 4CL1, pH and temperature not specified in the publication
5.22
cinnamate
isoform 4CL1, pH and temperature not specified in the publication
14.99
ferulate
isoform 4CL1, pH and temperature not specified in the publication
0.19
4-coumarate
isoform 4CL3, pH and temperature not specified in the publication
0.13
caffeate
isoform 4CL3, pH and temperature not specified in the publication
0.27
cinnamate
isoform 4CL3, pH and temperature not specified in the publication
0.15
ferulate
isoform 4CL3, pH and temperature not specified in the publication
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137.3
4-coumarate
isoform 4CL1, pH and temperature not specified in the publication
54.1
caffeate
isoform 4CL1, pH and temperature not specified in the publication
4.8
cinnamate
isoform 4CL1, pH and temperature not specified in the publication
287.4
ferulate
isoform 4CL1, pH and temperature not specified in the publication
17.3
4-coumarate
isoform 4CL3, pH and temperature not specified in the publication
3.1
caffeate
isoform 4CL3, pH and temperature not specified in the publication
0.3
cinnamate
isoform 4CL3, pH and temperature not specified in the publication
1.8
ferulate
isoform 4CL3, pH and temperature not specified in the publication
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loblolly pine
UniProt
brenda
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brenda
highest expression in root tip
brenda
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brenda
highest expression in root tip
brenda
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differentiating
brenda
additional information
isoform 4CL3 is absent in xylem
brenda
additional information
isoform 4CL3 is absent in xylem
brenda
additional information
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isoform 4CL3 is absent in xylem
brenda
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4CL_PINTA
537
0
58591
Swiss-Prot
other Location (Reliability: 2)
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His-Bind purification kit
His-Bind purification kit
recombinant protein using His-tag
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expressed in Escherichia coli Rosetta 2 (DE3) cells
60-66% sequence identity with 4-coumarate:CoA ligase sequences from herbaceous angiosperms
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expressed in Escherichia coli as His-tag fusion protein
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expressed in Escherichia coli Rosetta 2 (DE3) cells
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Zhang, X.H.; Chiang, V.L.
Molecular cloning of 4-coumarate:coenzyme A ligase in loblolly pine and the roles of this enzyme in the biosynthesis of lignin in compression wood
Plant Physiol.
113
65-74
1997
Pinus taeda
brenda
Voo, K.S.; Whetten, R.W.; O'Malley, D.M.; Sederoff, R.R.
4-Coumarate:coenzyme A ligase from loblolly pine xylem. Isolation characterization, and complementary DNA cloning
Plant Physiol.
108
85-97
1995
Pinus taeda
brenda
Harding, S.A.; Leshkevich, J.; Chiang, V.L.; Tsai, C.J.
Differential substrate inhibition couples kinetically distinct 4-coumarate: coenzyme A ligases with spatially distinct metabolic roles in quaking aspen
Plant Physiol.
128
428-438
2002
Pinus taeda, Populus tremuloides
brenda
Chen, H.Y.; Babst, B.A.; Nyamdari, B.; Hu, H.; Sykes, R.; Davis, M.F.; Harding, S.A.; Tsai, C.J.
Ectopic expression of a loblolly pine class II 4-coumarate CoA ligase alters soluble phenylpropanoid metabolism but not lignin biosynthesis in Populus
Plant Cell Physiol.
55
1669-1678
2014
Pinus taeda (P41636), Pinus taeda (U5MY99), Pinus taeda
brenda
Transporter Classification Database (TCDB):
4.C.1.1.7
6.2.1.12 4-coumarate-CoA ligase
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The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). 
