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Information on EC 5.6.1.3 - plus-end-directed kinesin ATPase

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EC Tree
IUBMB Comments
Kinesins are a family of motor proteins that move unidirectionally along microtubules as they hydrolyse ATP. The enzymes described here move towards the plus end of the microtubule, in contrast to EC 5.6.1.2, dynein ATPase and EC 5.6.1.4, minus-end-directed kinesin ATPase. They are involved in organelle movement in mitosis and meiosis, and also power vesicular trafficking toward the synapse in neurons. The motor domain, which contains the ATP- and microtubule-binding activities, is located at the N-terminus while the C-terminus links to the cargo being transported.
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UNIPROT: P52732
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Word Map
The enzyme appears in viruses and cellular organisms
Synonyms
kinesin, kinesin-1, kif3a, kinesin-5, kif1a, kif11, kif4a, kifc1, kinesin-2, kif5b, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
kinesin Eg5
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kinesin spindle protein
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kinesin-5 (Eg5/KSP) ATPase
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kinesin-like protein
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mitotic kinesin-5
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kinesin
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of phosphoric ester
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-
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SYSTEMATIC NAME
IUBMB Comments
kinesin ATP phosphohydrolase (plus-end-directed)
Kinesins are a family of motor proteins that move unidirectionally along microtubules as they hydrolyse ATP. The enzymes described here move towards the plus end of the microtubule, in contrast to EC 5.6.1.2, dynein ATPase and EC 5.6.1.4, minus-end-directed kinesin ATPase. They are involved in organelle movement in mitosis and meiosis, and also power vesicular trafficking toward the synapse in neurons. The motor domain, which contains the ATP- and microtubule-binding activities, is located at the N-terminus while the C-terminus links to the cargo being transported.
CAS REGISTRY NUMBER
COMMENTARY hide
9000-83-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + H2O + a kinesin associated with a microtubule at position n
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
show the reaction diagram
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-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + H2O + a kinesin associated with a microtubule at position n
ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
show the reaction diagram
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-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methanamine
-
(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)(1-methylpiperidin-3-yl)methanone
-
(2R)-2-amino-1-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-3-methylbutan-1-one
-
(2R,4aS,5R,10bS)-2-((1H-1,2,4-triazol-1-yl)methyl)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(2S)-2-amino-1-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-3-methylbutan-1-one
-
(4aS,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,10bS)-9-(tert-butyl)-5-(3-chlorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,10bS)-9-(tert-butyl)-5-(3-fluorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,10bS)-9-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline-9-carbonitrile
-
(4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline-9-carboxylic acid
-
(4aS,5R,10bS)-5-phenyl-9-(trifluoromethoxy)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-5-phenyl-9-propyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-7,9-dimethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-7-fluoro-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-8,10-dimethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-8,9-dimethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-8-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-8-chloro-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(pentafluoro-lambda6-sulfanyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-(1H-imidazol-2-yl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-(2-fluorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-(3-chlorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-(3-fluoro-4-methoxyphenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-(3-fluorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-(4-fluorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-(pyridin-3-yl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-(thiazol-2-yl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-chloro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-ethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-isopropyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-9-methyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
(4aS,5R,10bS)-N,N-dimethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-9-amine
-
(4aS,5S,10bS)-9-(tert-butyl)-5-cyclohexyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
-
1-((2R,4aS,5R,10bS)-5-(4-fluorophenyl)-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)-N-methylmethanamine
-
1-((2R,4aS,5R,10bS)-9-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)-N-methylmethanamine
-
1-((2R,4aS,5R,10bS)-9-bromo-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)-N-methylmethanamine
-
1-((2R,4aS,5R,10bS)-9-cyclopropyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)-N-methylmethanamine
-
1-(1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)ethanone
i.e. tetrahydro-beta-carboline
1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-3-(piperidin-1-yl)propan-1-one
-
1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)ethanone
-
1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
1-(2-(dimethylamino)ethyl)-3-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)urea
-
1-(3-hydroxypropyl)-3-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)urea
-
1-(4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenyl)ethanone
-
1-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-2-(piperidin-4-yl)ethanone
-
2-((((2R,4aS,5R,10bS)-9-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)(ethyl)amino)ethanol
-
2-((((2R,4aS,5R,10bS)-9-chloro-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)amino)ethanol
-
2-((2R,4aS,5R,10bS)-9-(tert-butyl)-2-((methylamino)methyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)benzenethiol
-
2-((4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-9-yl)acetonitrile
-
2-((4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-9-yl)ethanol
-
2-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)-4-chlorobenzenethiol
-
2-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)benzenethiol
-
2-(dimethylamino)ethyl (((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)carbamate
-
2-(methylamino)-N-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)ethanesulfonothioamide
-
2-amino-1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)ethanone
-
3-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)aniline
-
3-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)benzenethiol
-
3-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenol
-
3-(2-((2-aminoethyl)sulfonyl)-6-chloro-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indol-1-yl)phenol
-
3-(dimethylamino)-N-(((2R,4aS,5R,10bS)-9-isopropyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)propane-1-sulfonamide
-
3-amino-1-(1-(2-aminopyridin-4-yl)-6-chloro-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(1-(3-fluorophenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(1-(3-hydroxyphenyl)-6-(trifluoromethoxy)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(1-(3-hydroxyphenyl)-6-isopropyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(1-(3-hydroxyphenyl)-6-phenyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(1-(3-methoxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(6-bromo-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(6-chloro-1-cyclohexyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(6-ethyl-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(6-fluoro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(6-hydroxy-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-1-(6-methyl-1-phenyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
-
3-amino-N-methyl-N-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)propane-1-sulfonothioamide
-
4-((2R,4aS,5R,10bS)-2-((methylamino)methyl)-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenol
-
4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)-2-fluorophenol
-
4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)benzene-1,2-diol
-
4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenol
-
4-((4aS,5S,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)butan-1-ol
-
4-(2-[1-(2,4,6-trifluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
-
4-(2-[1-(2,4-difluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
-
4-(2-[1-(2,6-difluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
-
4-(2-[1-(2-fluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
-
4-(2-[1-(4-chlorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
-
4-(2-[1-(4-fluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
treatmebnt of cells results in inhibition of proliferation and induction of caspase 3 activity with EC50 values of 0.0017 and 0.0011 mM, respectively. Compound induces monoastral spindles in A2780 cells when incubated at concentrations above that required to induce a full mitotic arrest
4-amino-1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)butan-1-one
-
4-[2-(1-phenylcyclopropyl)-1,3-thiazol-4-yl]pyridine 1-oxide
-
5-(2-(3-aminopropanoyl)-6-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indol-1-yl)benzo[d]oxazol-2(3H)-one
-
5-[2-(1-phenylcyclopropyl)-1,3-thiazol-4-yl]pyridine-2-carbonitrile
-
N-(((2R,4aS,5R,10bS)-9-bromo-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)-4-(dimethylamino)butanamide
-
N-(((2R,4aS,5R,10bS)-9-chloro-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)-2-(dimethylamino)ethanesulfonamide
-
N-(2-((((2R,4aS,5R,10bS)-9-bromo-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)amino)ethyl)acetamide
-
N-(2-aminoethyl)-4-methyl-N-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)benzamide
-
N-(3-(2-(3-aminopropanoyl)-6-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indol-1-yl)phenyl)methanesulfonamide
-
N-(3-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-3-oxopropyl)methanesulfonamide
-
N-(4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenyl)acetamide
-
N1-phenyl-N1-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)ethane-1,2-diamine
-
PVZB1194
allosteric inhibitor, biphenyl-type inhibitor, binding structure of the inhibitor in complex with the Eg5 motor domain: inhibitor PVZB1194 binds to the alpha4/alpha6 allosteric pocket 15 A from the ATP-binding pocket, which differs from conventional allosteric inhibitors that bind to the allosteric L5/alpha2/alpha 3 pocket of Eg5. Binding of the inhibitor is involved in the neck-linker conformation and also causes conformational changes around the ATP-binding pocket through Tyr104 to affect the interaction of ATP with the pocket. Folding rearrangement of enzyme Eg5 induced by PVZB1194 in the absence of nucleotides and microtubules. Residue Tyr104 is involved in ATP-competitive inhibition by PVZB1194
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00045 - 0.054
ADP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00002
((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methanamine
Homo sapiens
pH and temperature not specified in the publication
0.0009
(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)(1-methylpiperidin-3-yl)methanone
Homo sapiens
pH and temperature not specified in the publication
0.0081
(2R)-2-amino-1-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-3-methylbutan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.000006
(2R,4aS,5R,10bS)-2-((1H-1,2,4-triazol-1-yl)methyl)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00076
(2S)-2-amino-1-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-3-methylbutan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.01
(4aS,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.01
(4aS,10bS)-9-(tert-butyl)-5-(3-chlorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.00087
(4aS,10bS)-9-(tert-butyl)-5-(3-fluorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.0003
(4aS,10bS)-9-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.01
(4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.01
(4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline-9-carbonitrile
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.01
(4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline-9-carboxylic acid
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.0037
(4aS,5R,10bS)-5-phenyl-9-(trifluoromethoxy)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00011
(4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00036
(4aS,5R,10bS)-5-phenyl-9-propyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.01
(4aS,5R,10bS)-7,9-dimethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.00009
(4aS,5R,10bS)-7-fluoro-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.01
(4aS,5R,10bS)-8,10-dimethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.00125
(4aS,5R,10bS)-8,9-dimethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.01
(4aS,5R,10bS)-8-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.00054
(4aS,5R,10bS)-8-chloro-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00091
(4aS,5R,10bS)-9-(pentafluoro-lambda6-sulfanyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.01
(4aS,5R,10bS)-9-(tert-butyl)-5-(1H-imidazol-2-yl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.00017
(4aS,5R,10bS)-9-(tert-butyl)-5-(2-fluorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.0068
(4aS,5R,10bS)-9-(tert-butyl)-5-(3-chlorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.01
(4aS,5R,10bS)-9-(tert-butyl)-5-(3-fluoro-4-methoxyphenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.00035
(4aS,5R,10bS)-9-(tert-butyl)-5-(3-fluorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00027
(4aS,5R,10bS)-9-(tert-butyl)-5-(4-fluorophenyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.0011
(4aS,5R,10bS)-9-(tert-butyl)-5-(pyridin-3-yl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00053
(4aS,5R,10bS)-9-(tert-butyl)-5-(thiazol-2-yl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00012
(4aS,5R,10bS)-9-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00072
(4aS,5R,10bS)-9-chloro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00013
(4aS,5R,10bS)-9-ethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00013
(4aS,5R,10bS)-9-isopropyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.00042
(4aS,5R,10bS)-9-methyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
pH and temperature not specified in the publication
0.0053
(4aS,5R,10bS)-N,N-dimethyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-9-amine
Homo sapiens
pH and temperature not specified in the publication
0.01
(4aS,5S,10bS)-9-(tert-butyl)-5-cyclohexyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinoline
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.000058
1-((2R,4aS,5R,10bS)-5-(4-fluorophenyl)-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)-N-methylmethanamine
Homo sapiens
pH and temperature not specified in the publication
0.000004
1-((2R,4aS,5R,10bS)-9-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)-N-methylmethanamine
Homo sapiens
pH and temperature not specified in the publication
0.000003
1-((2R,4aS,5R,10bS)-9-bromo-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)-N-methylmethanamine
Homo sapiens
pH and temperature not specified in the publication
0.000011
1-((2R,4aS,5R,10bS)-9-cyclopropyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)-N-methylmethanamine
Homo sapiens
pH and temperature not specified in the publication
0.0025
1-(1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)ethanone
Homo sapiens
i.e. tetrahydro-beta-carboline, pH and temperature not specified in the publication
0.0001
1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-3-(piperidin-1-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.0002
1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)ethanone
Homo sapiens
pH and temperature not specified in the publication
0.00046
1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.000008
1-(2-(dimethylamino)ethyl)-3-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)urea
Homo sapiens
i.e. EMD 534085, pH and temperature not specified in the publication
0.00001
1-(3-hydroxypropyl)-3-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)urea
Homo sapiens
pH and temperature not specified in the publication
0.01
1-(4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenyl)ethanone
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.00086
1-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-2-(piperidin-4-yl)ethanone
Homo sapiens
pH and temperature not specified in the publication
0.000019
2-((((2R,4aS,5R,10bS)-9-(tert-butyl)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)(ethyl)amino)ethanol
Homo sapiens
pH and temperature not specified in the publication
0.000007
2-((((2R,4aS,5R,10bS)-9-chloro-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)amino)ethanol
Homo sapiens
pH and temperature not specified in the publication
0.000005
2-((2R,4aS,5R,10bS)-9-(tert-butyl)-2-((methylamino)methyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)benzenethiol
Homo sapiens
pH and temperature not specified in the publication
0.0005
2-((4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-9-yl)acetonitrile
Homo sapiens
pH and temperature not specified in the publication
0.01
2-((4aS,5R,10bS)-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-9-yl)ethanol
Homo sapiens
pH and temperature not specified in the publication
0.0003
2-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)-4-chlorobenzenethiol
Homo sapiens
pH and temperature not specified in the publication
0.000025
2-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)benzenethiol
Homo sapiens
pH and temperature not specified in the publication
0.000014
2-(dimethylamino)ethyl (((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)carbamate
Homo sapiens
pH and temperature not specified in the publication
0.000004
2-(methylamino)-N-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)ethanesulfonothioamide
Homo sapiens
pH and temperature not specified in the publication
0.000049
2-amino-1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)ethanone
Homo sapiens
pH and temperature not specified in the publication
0.0023
3-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)aniline
Homo sapiens
pH and temperature not specified in the publication
0.00012
3-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)benzenethiol
Homo sapiens
pH and temperature not specified in the publication
0.00004
3-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenol
Homo sapiens
pH and temperature not specified in the publication
0.000186
3-(2-((2-aminoethyl)sulfonyl)-6-chloro-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indol-1-yl)phenol
Homo sapiens
pH and temperature not specified in the publication
0.000002
3-(dimethylamino)-N-(((2R,4aS,5R,10bS)-9-isopropyl-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)propane-1-sulfonamide
Homo sapiens
pH and temperature not specified in the publication
0.0066
3-amino-1-(1-(2-aminopyridin-4-yl)-6-chloro-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.0001
3-amino-1-(1-(3-fluorophenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.00063
3-amino-1-(1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.0002
3-amino-1-(1-(3-hydroxyphenyl)-6-(trifluoromethoxy)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.025
3-amino-1-(1-(3-hydroxyphenyl)-6-isopropyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
above 0.025 mM, pH and temperature not specified in the publication
0.00002 - 0.000058
3-amino-1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
0.0017
3-amino-1-(1-(3-hydroxyphenyl)-6-phenyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.011
3-amino-1-(1-(3-methoxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.000027
3-amino-1-(6-bromo-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.000039
3-amino-1-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.00023
3-amino-1-(6-chloro-1-cyclohexyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.000011
3-amino-1-(6-ethyl-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.00076
3-amino-1-(6-fluoro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.0045
3-amino-1-(6-hydroxy-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.000175
3-amino-1-(6-methyl-1-phenyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)propan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.000007
3-amino-N-methyl-N-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)propane-1-sulfonothioamide
Homo sapiens
pH and temperature not specified in the publication
0.000004
4-((2R,4aS,5R,10bS)-2-((methylamino)methyl)-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenol
Homo sapiens
pH and temperature not specified in the publication
0.000017
4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)-2-fluorophenol
Homo sapiens
pH and temperature not specified in the publication
0.000095
4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)benzene-1,2-diol
Homo sapiens
pH and temperature not specified in the publication
0.000034
4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenol
Homo sapiens
pH and temperature not specified in the publication
0.01
4-((4aS,5S,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)butan-1-ol
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.00023
4-(2-[1-(2,4,6-trifluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
Homo sapiens
-
0.00033
4-(2-[1-(2,4-difluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
Homo sapiens
-
0.00028
4-(2-[1-(2,6-difluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
Homo sapiens
-
0.0003
4-(2-[1-(2-fluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
Homo sapiens
-
0.00029
4-(2-[1-(4-chlorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
Homo sapiens
-
0.00054
4-(2-[1-(4-fluorophenyl)cyclopropyl]-1,3-thiazol-4-yl)pyridine
Homo sapiens
pH 6.8, 22°C
0.000086
4-amino-1-(1-(3-hydroxyphenyl)-6-methyl-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)butan-1-one
Homo sapiens
pH and temperature not specified in the publication
0.00047
4-[2-(1-phenylcyclopropyl)-1,3-thiazol-4-yl]pyridine 1-oxide
Homo sapiens
-
0.025
5-(2-(3-aminopropanoyl)-6-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indol-1-yl)benzo[d]oxazol-2(3H)-one
Homo sapiens
above 0.025 mM, pH and temperature not specified in the publication
0.000532
5-[2-(1-phenylcyclopropyl)-1,3-thiazol-4-yl]pyridine-2-carbonitrile
Homo sapiens
-
0.000005
N-(((2R,4aS,5R,10bS)-9-bromo-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)-4-(dimethylamino)butanamide
Homo sapiens
pH and temperature not specified in the publication
0.000002
N-(((2R,4aS,5R,10bS)-9-chloro-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)-2-(dimethylamino)ethanesulfonamide
Homo sapiens
pH and temperature not specified in the publication
0.000006
N-(2-((((2R,4aS,5R,10bS)-9-bromo-7-fluoro-5-phenyl-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)amino)ethyl)acetamide
Homo sapiens
pH and temperature not specified in the publication
0.000065
N-(2-aminoethyl)-4-methyl-N-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)benzamide
Homo sapiens
pH and temperature not specified in the publication
0.025
N-(3-(2-(3-aminopropanoyl)-6-methyl-2,3,4,9-tetrahydro-1H-pyrido[3,4-b]indol-1-yl)phenyl)methanesulfonamide
Homo sapiens
above 0.025 mM, pH and temperature not specified in the publication
0.000155
N-(3-(6-chloro-1-(3-hydroxyphenyl)-3,4-dihydro-1H-pyrido[3,4-b]indol-2(9H)-yl)-3-oxopropyl)methanesulfonamide
Homo sapiens
pH and temperature not specified in the publication
0.01
N-(4-((4aS,5R,10bS)-9-(tert-butyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-5-yl)phenyl)acetamide
Homo sapiens
above 0.01 mM, pH and temperature not specified in the publication
0.000019
N1-phenyl-N1-(((2R,4aS,5R,10bS)-5-phenyl-9-(trifluoromethyl)-3,4,4a,5,6,10b-hexahydro-2H-pyrano[3,2-c]quinolin-2-yl)methyl)ethane-1,2-diamine
Homo sapiens
pH and temperature not specified in the publication
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22
assay at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
paclitaxel-stabilized microtubules are used for activity assays
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme is a member of the kinesin-5 family
malfunction
inhibition of Eg5 causes cell cycle arrest in mitosis with the irregular formation of monopolar spindles and subsequent apoptotic cell death
physiological function
additional information
analysis of the molecular mechanism responsible for regulating the motor activity of kinesins, it plays an essential role in centrosome separation and bipolar mitotic spindle formation during the early stage of mitosis
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KIF11_HUMAN
1056
0
119159
Swiss-Prot
Secretory Pathway (Reliability: 4)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant Eg5 motor domain, residues 1-369, in complex with inhibitor PVZB1194, sitting drop vapor diffusion method, at 4°C, mixing of 500 nl of 6 mg/ml protein in 50 mM PIPES-NaOH, pH 6.8, 250 mM NaCl, 2 mM MgCl2, 1 mM EGTA-NaOH, 1 mM TCEP-HCl, and 10% w/v sucrose, and inhibitor PVZB1194 (ratio 1:3 or 1:4 with enzyme) solution with 500 nl of the reservoir solution containing 19% w/v PEG 3350, 0.1 M MES-NaOH, pH 6.0, and 200 mM NaNO3, 1 week, followed by microseeding, X-ray diffraction structure determination and analysis at 2.8 A resolution
study of the discovery and optimization of hexahydr-2H-pyranol[3,2-c]quinolines, HHPQs as inhibitors. Crystallographic data demonstrate that these potent and selectve inhibitors bind in an allosteric pocket of kinesin-5 distant from the nucleotide and microtubule binding sites
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R234K
motor domain amino acid residues 1-368, overall similar ATP affinity
S233C/R234K
motor domain amino acid residues 1-368, no ATP hydrolyzing mutant
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography (Ni2+)
recombinant C-terminally His6-tagged wild-type Eg51-369 and mutants from Escherichia coli BL21(DE3)pLysS by nickel affinity and cation exchange chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene KIF11, recombinant expression of C-terminally His6-tagged wild-type Eg51-369 and mutants in Escherichia coli BL21(DE3)pLysS
His tagged motor domains, amino acid residues 1-368 and 1-358, expressed in Escherichia coli B834(DE3)
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
inhibition of mitotic spindles is a pharmaceutically validated strategy for cancer therapeutics, human Eg5 in addition to microtubules represents an attractive target molecule for novel clinical therapies in the treatment of human malignancies, kinesin spindle protein Eg5 is a target for anticancer therapies
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Rickert, K.W.; Schaber, M.; Torrent, M.; Neilson, L.A.; Tasber, E.S.; Garbaccio, R.; Coleman, P.J.; Harvey, D.; Zhang, Y.; Yang, Y.; Marshall, G.; Lee, L.; Walsh, E.S.; Hamilton, K.; Buser, C.A.
Discovery and biochemical characterization of selective ATP competitive inhibitors of the human mitotic kinesin KSP
Arch. Biochem. Biophys.
469
220-231
2008
Homo sapiens (P52732), Homo sapiens
Manually annotated by BRENDA team
Schiemann, K.; Finsinger, D.; Zenke, F.; Amendt, C.; Knoechel, T.; Bruge, D.; Buchstaller, H.P.; Emde, U.; Staehle, W.; Anzali, S.
The discovery and optimization of hexahydro-2H-pyrano[3,2-c]quinolines (HHPQs) as potent and selective inhibitors of the mitotic kinesin-5
Bioorg. Med. Chem. Lett.
20
1491-1495
2010
Homo sapiens (P52732), Homo sapiens
Manually annotated by BRENDA team
Barsanti, P.A.; Wang, W.; Ni, Z.J.; Duhl, D.; Brammeier, N.; Martin, E.; Bussiere, D.; Walter, A.O.
The discovery of tetrahydro-beta-carbolines as inhibitors of the kinesin Eg5
Bioorg. Med. Chem. Lett.
20
157-160
2010
Homo sapiens (P52732)
Manually annotated by BRENDA team
Zhao, Y.C.; Kull, F.J.; Cochran, J.C.
Modulation of the kinesin ATPase cycle by neck linker docking and microtubule binding
J. Biol. Chem.
285
25213-25220
2010
Homo sapiens (P52732), Homo sapiens
Manually annotated by BRENDA team
Yokoyama, H.; Sawada, J.; Katoh, S.; Matsuno, K.; Ogo, N.; Ishikawa, Y.; Hashimoto, H.; Fujii, S.; Asai, A.
Structural basis of new allosteric inhibition in kinesin spindle protein Eg5
ACS Chem. Biol.
10
1128-1136
2015
Homo sapiens (P52732)
Manually annotated by BRENDA team