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Literature summary for 5.6.1.3 extracted from

  • Zhao, Y.C.; Kull, F.J.; Cochran, J.C.
    Modulation of the kinesin ATPase cycle by neck linker docking and microtubule binding (2010), J. Biol. Chem., 285, 25213-25220.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.1.3

Cloned(Commentary)

Cloned (Comment) Organism
His tagged motor domains, amino acid residues 1-368 and 1-358, expressed in Escherichia coli B834(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
R234K motor domain amino acid residues 1-368, overall similar ATP affinity Homo sapiens
S233C/R234K motor domain amino acid residues 1-368, no ATP hydrolyzing mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P52732
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Homo sapiens ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Synonyms

Synonyms Comment Organism
kinesin-5 (Eg5/KSP) ATPase
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00045
-
 ADP motor domain (amino acid residues 1-358), competitive inhibition of 2'-(3')-O-(N-methylanthraniloyl)ATP binding in the absence of microtubles, pH 7.2, temperature not specified in the publication Homo sapiens
0.0033
-
 ADP motor domain (amino acid residues 1-368), competitive inhibition of 2'-(3')-O-(N-methylanthraniloyl)ATP binding in the absence of microtubles, pH 7.2, temperature not specified in the publication Homo sapiens
0.048
-
 ADP motor domain (amino acid residues 1-368), competitive inhibition of 2'-(3')-O-(N-methylanthraniloyl)ATP binding in the presence of microtubles, pH 7.2, temperature not specified in the publication Homo sapiens
0.054
-
 ADP motor domain (amino acid residues 1-358), competitive inhibition of 2'-(3')-O-(N-methylanthraniloyl)ATP binding in the presence of microtubles, pH 7.2, temperature not specified in the publication Homo sapiens