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Information on EC 5.5.1.13 - ent-copalyl diphosphate synthase and Organism(s) Phaeosphaeria sp. and UniProt Accession O13284

for references in articles please use BRENDA:EC5.5.1.13
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EC Tree
IUBMB Comments
Part of a bifunctional enzyme involved in the biosynthesis of kaurene. See also EC 4.2.3.19 (ent-kaurene synthase)
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This record set is specific for:
Phaeosphaeria sp.
UNIPROT: O13284
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Word Map
  • 5.5.1.13
  • gibberellin
  • diterpene
  • diterpenoids
  • geranylgeranyl
  • phytohormone
  • phytoalexins
  • ent-cpp
  • ent-kaurenoic
  • phytocassanes
  • e,e,e-geranylgeranyl
  • oscps4
  • 3-oxidase
  • ga-deficient
  • labdane-related
  • ga1
  • ditpss
  • oryzalexins
  • ga20ox
  • syn-copalyl
The taxonomic range for the selected organisms is: Phaeosphaeria sp.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
ent-copalyl diphosphate synthase, oscps1, ent-cps, ent-kaurene synthase a, oscyc2, gfcps/ks, oscps2ent, ent-kaurene synthetase a, copalyl synthase/kaurene synthase, oscps1ent, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ent-kaurene synthase A
-
-
-
-
ent-kaurene synthetase A
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
intramolecular lyase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ent-copalyl-diphosphate lyase (decyclizing)
Part of a bifunctional enzyme involved in the biosynthesis of kaurene. See also EC 4.2.3.19 (ent-kaurene synthase)
CAS REGISTRY NUMBER
COMMENTARY hide
9055-64-5
in Chemical Abstracts not distinguished from EC 4.2.3.19
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
copalyl diphosphate
ent-kaurene + diphosphate
show the reaction diagram
geranylgeranyl diphosphate
copalyl diphosphate
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl-piperidine-1-carboxylate
AMO-1618, a quaternary ammonium salt, leads to total enzyme inhibition at 0.001 mM
EDTA
inhibits activity in cell free extract containing the enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00023
copalyl diphosphate
at pH 8
0.00873
geranylgeranyl diphosphate
at pH 8
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00117
copalyl diphosphate
at pH 8
0.0308
geranylgeranyl diphosphate
at pH 8
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00075
at pH 8
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
from mycelia
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
106000
calculated from amino acid sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D320A
the mutation leads to complete enzyme activity
D656A
the mutation causes a small reduction in activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cell free extract
recombinant enzyme, using Sepharose 4B glutathione affinity resin and SDS PAGE electrophoresis
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli of a recombinant GST fused enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kawaide, H.; Imai, R.; Sassa, T.; Kamiya, Y.
ent-Kaurene synthetase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis
J. Biol. Chem.
272
21706-21712
1997
Phaeosphaeria sp. (O13284)
Manually annotated by BRENDA team
Kawaide, H.; Sassa, T.; Kamiya, Y.
Functional analysis of the two interacting cyclase domains in ent-kaurene synthase from the fungus Phaeosphaeria sp. L487 and a comparison with cyclases from higher plants
J. Biol. Chem.
275
2276-2280
2000
Cucurbita maxima, Phaeosphaeria sp. (O13284)
Manually annotated by BRENDA team