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Information on EC 5.4.99.39 - beta-amyrin synthase and Organism(s) Panax ginseng and UniProt Accession O82140

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EC Tree
     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.39 beta-amyrin synthase
IUBMB Comments
Some organism possess a monofunctional beta-amyrin synthase [3,4,6-11], other have a multifunctional enzyme that also catalyses the synthesis of alpha-amyrin (EC 5.4.99.40) or lupeol (EC 5.4.99.41) .
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This record set is specific for:
Panax ginseng
UNIPROT: O82140
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Word Map
The taxonomic range for the selected organisms is: Panax ginseng
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-amyrin synthase, beta-as, mdosc1, gsas1, gsas2, esbas, cbbas, multifunctional osc, beta-amyrin cyclase, obas1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
(3S)-2,3-epoxy-2,3-dihydrosqualene mutase (cyclizing, beta-amyrin-forming)
Some organism possess a monofunctional beta-amyrin synthase [3,4,6-11], other have a multifunctional enzyme that also catalyses the synthesis of alpha-amyrin (EC 5.4.99.40) [5] or lupeol (EC 5.4.99.41) [6].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,3-oxidosqualene
beta-amyrin
show the reaction diagram
-
-
-
?
(S)-2,3-oxidosqualene
beta-amyrin
show the reaction diagram
-
-
-
-
?
2,3-oxidosqualene
beta-amyrin
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
hairy root
Manually annotated by BRENDA team
-
adventitious roots
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BAMS1_PANGI
763
0
87994
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
88000
x * 88000, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 88000, calculated
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C262S
-
mutation in region responsible for product differentiation. Like wild-type, mutant produces beta-amyrin as sole product
M258I/W259L
-
mutation in region responsible for product differentiation. Mutant produces 40.5% beta-amyrin, 53.4% lupeol, 3.6% butyrospermol and 2.5% germanicol
W259L
-
mutation in region responsible for product differentiation. Mutant produces 30.3% beta-amyrin, 54.6% lupeol, 3.6% butyrospermol and 3.4% germanicol
Y261H
-
mutation in region responsible for product differentiation. Mutant produces 2.4% lupeol, 13.6% germanicol and 84% of dammara-18(E),21-dien-3beta-ol, dammara-18(Z),21-dien-3beta-ol and dammara-18(28),21-dien-3beta-ol
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisae
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kushiro, T.; Shibuya, M.; Ebizuka, Y.
beta-Amyrin synthase - cloning of oxidosqualene cyclase that catalyzes the formation of the most popular triterpene among higher plants
Eur. J. Biochem.
256
238-244
1998
Panax ginseng (O82140), Panax ginseng
Manually annotated by BRENDA team
Kushiro, T.; Shibuya, M.; Masuda, K.; Ebizuka, Y.
Mutational studies on triterpene synthases: engineering lupeol synthase into beta-amyrin synthase
J. Am. Chem. Soc.
122
6816-6824
2000
Panax ginseng
-
Manually annotated by BRENDA team
Yendo, A.C.; de Costa, F.; Gosmann, G.; Fett-Neto, A.G.
Production of plant bioactive triterpenoid saponins: elicitation strategies and target genes to improve yields
Mol. Biotechnol.
46
94-104
2010
Medicago truncatula, Gypsophila vaccaria, Centella asiatica, Panax ginseng, Aster sedifolius, Gentiana straminea
Manually annotated by BRENDA team