EC Number |
Protein Variants |
Reference |
---|
5.4.99.39 | C262S |
mutation in region responsible for product differentiation. Like wild-type, mutant produces beta-amyrin as sole product |
683581 |
5.4.99.39 | C486A |
mutant shows 46% activity compared to wild-type (100%), Km similar to wild-type, kcat decreased |
729707 |
5.4.99.39 | C564A |
mutant shows 1.6% activity compared to wild-type (100%), Km increased compared to wild-type, kcat highly decreased |
729707 |
5.4.99.39 | D485E |
mutant shows no activity |
729707 |
5.4.99.39 | D485N |
mutant shows no activity |
729707 |
5.4.99.39 | F413A |
site-directed mutagenesis, functional analysis, the mutant shows slightly reduced activity compared to thre wild-type enzyme |
748812 |
5.4.99.39 | F413H |
site-directed mutagenesis, functional analysis, the mutant shows slightly increased activity compared to thre wild-type enzyme |
748812 |
5.4.99.39 | F413M |
site-directed mutagenesis, functional analysis, the mutant shows slightly reduced activity compared to thre wild-type enzyme |
748812 |
5.4.99.39 | F413S |
site-directed mutagenesis, functional analysis, the mutant shows slightly increased activity compared to thre wild-type enzyme |
748812 |
5.4.99.39 | F413T |
site-directed mutagenesis, functional analysis, the mutant shows slightly increased activity compared to thre wild-type enzyme |
748812 |