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Information on EC 5.4.99.24 - 23S rRNA pseudouridine955/2504/2580 synthase and Organism(s) Escherichia coli and UniProt Accession P0AA39

for references in articles please use BRENDA:EC5.4.99.24
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IUBMB Comments
The enzyme converts uridines at position 955, 2504 and 2580 of 23S rRNA to pseudouridines.
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This record set is specific for:
Escherichia coli
UNIPROT: P0AA39
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
23S rRNA uridine955/uridine2504/uridine2580
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23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
Synonyms
pseudouridine synthases RluC, ribosomal large subunit pseudouridine synthase C, RLuc, YceC, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pseudouridine synthases RluC
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ribosomal large subunit pseudouridine synthase C
UniProt
SYSTEMATIC NAME
IUBMB Comments
23S rRNA-uridine955/2504/2580 uracil mutase
The enzyme converts uridines at position 955, 2504 and 2580 of 23S rRNA to pseudouridines.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
show the reaction diagram
23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
show the reaction diagram
-
-
-
?
23S rRNA uridine955/uridine2504/uridine2580
23S rRNA pseudouridine955/pseudouridine2504/pseudouridine2580
show the reaction diagram
-
-
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
RluC is associates with a pre-50S ribosomal particle
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
malfunction
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
analytical ultracentrifugation sedimentation velocity experiments show that RluC(92-319) is monomeric in solution
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a proteolytically derived fragment of the enzyme consisting of residues 89-319 retains catalytic activity. Crystals of this fragment, grown by precipitation with sodium acetate at pH 8.0, belong to space group P321, with unit-cell dimensions a = b = 97.1, c = 86.3 A and have two molecules in the crystallographic asymmetric unit. Crystals diffract X-rays to at least 2.3 A resolution and appear suitable for crystal structure determination
crystals of the catalytic domain RluC(92-319) are grown at 20°C using the hanging drop method
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D139N
site-directed mutagenesis of the chimeric enzyme mutant RluCD, the mutant is catalytically inactive
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant expression of chimeric enzyme mutants RluCD in Escherichia coli strains M15 and CD204, expression of chimeric pseudouridine synthase RluCD, under control of arabinose inducible araBAD promoter, interferes with ribosome formation in wild-type M15 strain and strain CD204 lacking RluC and RluD, while cells transformed with catalytically inactive RluCD (RluCD D139N) and empty vector (pQE60), used as a controls, represent a normal ribosomal profile in sucrose density gradient. Strain CD204 contains a mutant form of RF-2 (D131Y) that suppresses ribosome assembly defect caused by the deletion of the RluD. Strain rluD114 where yfiI (RluD) gene has been disrupted with Km cassette is used as a parent strain to construct strain CD204
overexpressed in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Corollo, D.; Blair-Johnson, M.; Conrad, J.; Friedler, T.; Sun, D.; Wang, L.; Ofengand, J.; Fenna, R.
Crystallisation and characterization of a fragment of pseudouridine synthase RluC from Escherichia coli
Acta Crystallogr. Sect. D
55
302-304
1999
Escherichia coli (P0AA39)
-
Manually annotated by BRENDA team
Toh, S.M.; Mankin, A.S.
An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors
J. Mol. Biol.
380
593-597
2008
Escherichia coli
Manually annotated by BRENDA team
Huang, L.; Ku, J.; Pookanjanatavip, M.; Gu, X.; Wang, D.; Greene, P.J.; Santi, D.V.
Identification of two Escherichia coli pseudouridine synthases that show multisite specificity for 23S RNA
Biochemistry
37
15951-15957
1998
Escherichia coli
Manually annotated by BRENDA team
Mizutani, K.; Machida, Y.; Unzai, S.; Park, S.Y.; Tame, J.R.
Crystal structures of the catalytic domains of pseudouridine synthases RluC and RluD from Escherichia coli
Biochemistry
43
4454-4463
2004
Escherichia coli (P0AA39), Escherichia coli
Manually annotated by BRENDA team
Jiang, M.; Sullivan, S.M.; Walker, A.K.; Strahler, J.R.; Andrews, P.C.; Maddock, J.R.
Identification of novel Escherichia coli ribosome-associated proteins using isobaric tags and multidimensional protein identification techniques
J. Bacteriol.
189
3434-3444
2007
Escherichia coli
Manually annotated by BRENDA team
Conrad, J.; Sun, D.; Englund, N.; Ofengand, J.
The rluC gene of Escherichia coli codes for a pseudouridine synthase that is solely responsible for synthesis of pseudouridine at positions 955, 2504, and 2580 in 23 S ribosomal RNA
J. Biol. Chem.
273
18562-18566
1998
Escherichia coli (P0AA39)
Manually annotated by BRENDA team
Leppik, M.; Liiv, A.; Remme, J.
Random pseuoduridylation in vivo reveals critical region of Escherichia coli 23S rRNA for ribosome assembly
Nucleic Acids Res.
45
6098-6108
2017
Escherichia coli (P0AA39)
Manually annotated by BRENDA team