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Information on EC 5.3.1.1 - triose-phosphate isomerase and Organism(s) Giardia intestinalis and UniProt Accession P36186

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Giardia intestinalis
UNIPROT: P36186 not found.
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The taxonomic range for the selected organisms is: Giardia intestinalis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
triosephosphate isomerase, triose phosphate isomerase, triose-phosphate isomerase, tctim, pftim, gltim, monotim, pfutim, cp 25, cytoplasmic tpi, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Triosephosphate isomerase
-
CP 25
-
-
-
-
D-glyceraldehyde-3-phosphate ketol-isomerase
-
-
-
-
Isomerase, triose phosphate
-
-
-
-
Lactacin B inducer protein
-
-
-
-
monoTIM
-
-
-
-
PfTIM
-
-
-
-
Phosphotriose isomerase
-
-
-
-
TIM
-
-
-
-
Triose phosphate isomerase
-
-
-
-
Triose phosphate mutase
-
-
-
-
Triose phosphoisomerase
-
-
-
-
Triosephosphate isomerase
-
-
-
-
Triosephosphate mutase
-
-
-
-
vTIM
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
-
-
-
-
intramolecular oxidoreduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
D-glyceraldehyde-3-phosphate aldose-ketose-isomerase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9023-78-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
show the reaction diagram
D-glyceraldehyde 3-phosphate
dihydroxyacetone phosphate
show the reaction diagram
-
-
-
-
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-carboxyethyl methanethiosulfonate
modifies four Cys per subunit of dimeric protein and induces 97% of inactivation. Inactivation does not affect secondary structure nor induce dimer dissociation. Cys modification decreases thermal stability of the enzyme
5,5'-dithio-bis(2-nitrobenzoic acid)
derivatizes four of the five Cys per subunit of dimeric protein, resulting in inactivation and dissociation of the dimer to stable monomers
bromohydroxyacetone phosphate
suicide inhibitor
DL-glycidol phosphate
suicide inhibitor
methyl methanethiosulfonate
derivatizes three of the five Cys per subunit of dimeric protein and induces 50% of inactivation. Inactivation does not affect secondary structure nor induce dimer dissociation. Cys modification decreases thermal stability of the enzyme
2-Phosphoglycolate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.28 - 8.6
D-glyceraldehyde 3-phosphate
0.53 - 0.87
D-glyceraldehyde 3-phosphate
1.1
dihydroxyacetone phosphate
-
25°C, pH 7.4, dimeric enzyme form
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1666 - 8000
D-glyceraldehyde 3-phosphate
2450 - 4833
D-glyceraldehyde 3-phosphate
267
dihydroxyacetone phosphate
-
25°C, pH 7.4, dimeric enzyme form
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.043
2-Phosphoglycolate
-
25°C, pH 7.4, dimeric enzyme form
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
optimum is near pH 8.0
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPI1_GIAIN
257
0
27903
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
108000
-
tetramer, gel filtration
27000
55000
-
dimer, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
X-ray crystallography, only the TIM dimer is fully active
dimer
-
2 * 27000, SDS-PAGE
tetramer
-
4 * 27000, SDS-PAGE
additional information
-
tetramer results from covalent attachment of two dimers that conserve similar association constants between their constituent monomers
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
determination of intrinsic fluorescence of wild-type and mutants lacking Trp residues, interpretation based on crystal structure. Fluorescence of all Trp residues is quenched by aromatic-aromatic interactions due to the proximity and orientation of the indole groups of Trp196 and Tro162. Quenching is also due to energy transfer to the charged resiudes that surround Trp75, Trp173, and Trp196
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F12W
mutant constructed for fluorescence studies, catalytic properties similar to wild-type
W162F
mutant constructed for fluorescence studies, catalytic properties similar to wild-type
W162F/W173F/W196F
mutant constructed for fluorescence studies, catalytic properties similar to wild-type
W173F
mutant constructed for fluorescence studies, catalytic properties similar to wild-type
W196F
mutant constructed for fluorescence studies, catalytic properties similar to wild-type
W75F
mutant constructed for fluorescence quenching studies, catalytic properties similar to wild-type
W75F/W162F/W173F
mutant constructed for fluorescence studies, catalytic properties similar to wild-type
W75F/W162F/W196F
mutant constructed for fluorescence studies, catalytic properties similar to wild-type
W75F/W173F/W196F
mutant constructed for fluorescence studies, catalytic properties similar to wild-type
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli TOP10
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lopez-Velazquez, G.; Molina-Ortiz, D.; Cabrera, N.; Hernandez-Alcantara, G.; Peon-Peralta, J.; Yepez-Mulia, L.; Perez-Montfort, R.; Reyes-Vivas, H.
An unusual triosephosphate isomerase from the early divergent eukaryote Giardia lamblia
Proteins
55
824-834
2004
Giardia intestinalis
Manually annotated by BRENDA team
Hernandez-Alcantara, G.; Rodriguez-Romero, A.; Reyes-Vivas, H.; Peon, J.; Cabrera, N.; Ortiz, C.; Enriquez-Flores, S.; De la Mora-De la Mora, I.; Lopez-Velazquez, G.
Unraveling the mechanisms of tryptophan fluorescence quenching in the triosephosphate isomerase from Giardia lamblia
Biochim. Biophys. Acta
1784
1493-1500
2008
Giardia intestinalis (P36186), Giardia intestinalis
Manually annotated by BRENDA team
Enriquez-Flores, S.; Rodriguez-Romero, A.; Hernandez-Alcantara, G.; De la Mora-De la Mora, I.; Gutierrez-Castrellon, P.; Carvajal, K.; Lopez-Velazquez, G.; Reyes-Vivas, H.
Species-specific inhibition of Giardia lamblia triosephosphate isomerase by localized perturbation of the homodimer
Mol. Biochem. Parasitol.
157
179-186
2008
Giardia intestinalis (P36186), Giardia intestinalis
Manually annotated by BRENDA team
Wierenga, R.K.; Kapetaniou, E.G.; Venkatesan, R.
Triosephosphate isomerase: a highly evolved biocatalyst
Cell. Mol. Life Sci.
67
3961-3982
2010
Entamoeba histolytica (O02611), Oryctolagus cuniculus (P00939), Gallus gallus (P00940), Saccharomyces cerevisiae (P00942), Geobacillus stearothermophilus (P00943), Trypanosoma brucei brucei (P04789), Escherichia coli (P0A858), Giardia intestinalis (P36186), Thermotoga maritima (P36204), Leishmania mexicana (P48499), Moritella marina (P50921), Trypanosoma cruzi (P52270), Helicobacter pylori (P56076), Homo sapiens (P60174), Pyrococcus woesei (P62003), Mycobacterium tuberculosis (P9WG43), Plasmodium falciparum (Q07412), Caenorhabditis elegans (Q10657), Methanocaldococcus jannaschii (Q58923), Bartonella henselae (Q8L1Z5), Tenebrio molitor (Q8MPF2), Thermoproteus tenax (Q8NKN9), Mycobacterium tuberculosis H37Rv (P9WG43)
Manually annotated by BRENDA team