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Information on EC 5.1.3.20 - ADP-glyceromanno-heptose 6-epimerase and Organism(s) Burkholderia thailandensis and UniProt Accession Q2SY18

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IUBMB Comments
Requires NAD+.
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This record set is specific for:
Burkholderia thailandensis
UNIPROT: Q2SY18
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The taxonomic range for the selected organisms is: Burkholderia thailandensis
The enzyme appears in selected viruses and cellular organisms
Synonyms
hp0859, adp-l-glycero-d-mannoheptose-6-epimerase, adp-l-glycero-d-manno-heptose 6-epimerase, adp-l-glycero-d-manno-heptose-6-epimerase, adp-l-glycero-d-mannoheptose 6-epimerase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ADP-L-glycero-D-manno-heptose 6-epimerase
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Adenosine diphosphoglyceromannoheptose 6-epimerase
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ADP-glyceromanno-heptose 6-epimerase
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ADP-L-glycero-D-manno-heptose-6-epimerase
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ADP-L-glycero-D-manno-heptose-epimerase, Aquifex aelicis gene rfaD
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ADP-L-glycero-D-mannoheptose 6-epimerase
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ADP-L-glycero-D-mannoheptose 6-epimerase (Neisseria gonorrhoeae gene gme)
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ADP-L-glycero-D-mannoheptose-6-epimerase
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ADP-L-glycero-D-mannoheptose-6-epimerase (Escherichia coli strain K-12 substrain MG1655 clone EC19-98 gene rfaD)
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Epimerase, adenosine diphosphoglyceromannoheptose 6-
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Epimerase, adenosine diphosphoglyceromannoheptose 6- (Escherichia coli clone pCG50 reduced)
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Epimerase, adenosine diphosphoglyceromannoheptose 6- (Neisseria gonorrhoeae gene lis-6)
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Genbank AE000440-derived protein GI 1790049
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GenBank AE000684-derived protein GI 2983012
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP-D-glycero-D-manno-heptose = ADP-L-glycero-D-manno-heptose
show the reaction diagram
catalytic one-base reaction mechanism from docking study, overview. The key point in the catalytic process of AGME is the inversion at the C6'' stereocentre of ADP-D,D-Hep mediated by the reversible redox reactions of NADP+/NADPH
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
epimerization
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
ADP-L-glycero-D-manno-heptose 6-epimerase
Requires NAD+.
CAS REGISTRY NUMBER
COMMENTARY hide
130527-81-0
epimerase, adenosine diphosphoglyceromannoheptose 6- (Escherichia coli clone pCG50 reduced) /ADP-L-glycero-D-mannoheptose-6-epimerase (Escherichia coli strain K-12 substrain MG1655 clone EC19-98 gene rfaD) /genBank AE000440-derived protein GI 1790049
167617-20-1
epimerase, adenosine diphosphoglyceromannoheptose 6- (Neisseria gonorrhoeae gene lis-6), ADP-L-glycero-D-mannoheptose 6-epimerase (Neisseria gonorrhoeae gene gme)
204790-62-5
ADP-L-glycero-D-manno-heptose-epimerase, Aquifex aelicis gene rfaD /GenBank AE000684-derived protein GI 2983012
85030-75-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-D-glycero-D-manno-heptose
ADP-L-glycero-D-manno-heptose
show the reaction diagram
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r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP-D-glycero-D-manno-heptose
ADP-L-glycero-D-manno-heptose
show the reaction diagram
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r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADP+
the enzyme contains a catalytic triad involved in catalyzing hydride transfer with the aid of NADP+
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene rfaD
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
x * 38000, recombinant His6-tagged enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 38000, recombinant His6-tagged enzyme, SDS-PAGE
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, mixing of 200 nl of 8.8 mg/ml protein in 0.43 M NaCl, 20 mM Tris-HCl, pH 8.0, with 200 nl of reservoir solution containing 35% PEG 200, 0.1 M bis-tris-HCl, pH 5.5, and equilibration against 0.05 ml of reservori solution, method optimization, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular replacement
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E210G
site-directed mutagenesis, structure compared to the wild-type enzyme
K178M
site-directed mutagenesis, structure compared to the wild-type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene rfaD, sequence compariosns, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kim, M.; Lim, A.; Yang, S.; Park, J.; Lee, D.; Shin, D.
Structure and in silico substrate-binding mode of ADP-L-glycero-D-manno-heptose 6-epimerase from Burkholderia thailandensis
Acta Crystallogr. Sect. D
69
658-668
2013
Burkholderia thailandensis (Q2SY18), Burkholderia thailandensis, Burkholderia thailandensis E444 (Q2SY18)
Manually annotated by BRENDA team