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Information on EC 5.1.3.11 - cellobiose epimerase and Organism(s) Bacteroides fragilis and UniProt Accession Q5LH66
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5.1.3.11 cellobiose epimeraseIUBMB Comments
The enzyme catalyses the interconversion between D-glucose and D-mannose residues at the reducing end of beta-1,4-linked disaccharides by epimerizing the hydroxyl group at the C-2 position of the glucose moiety.
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Word Map
- 5.1.3.11
- synthesis
-
epimerization
- lactulose
- caldicellulosiruptor
- saccharolyticus
-
isomerization
- d-glucose
- ruminococcus
- albus
-
prebiotic
- d-mannose
- marinus
- turgidum
-
rhodothermus
- nutrition
-
dictyoglomus
- mannans
- food industry
- n-acyl-d-glucosamine
-
epimerizes
-
isomerizes
- cello-oligosaccharides
-
spirochaeta
- pharmacology
The taxonomic range for the selected organisms is: Bacteroides fragilis
The expected taxonomic range for this enzyme is: Bacteria, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
cellobiose 2-epimerase, mannobiose 2-epimerase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Epimerase, cellobiose
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose
reaction mechansim, overview
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
epimerization
-
-
-
-
PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
cellobiose 2-epimerase
The enzyme catalyses the interconversion between D-glucose and D-mannose residues at the reducing end of beta-1,4-linked disaccharides by epimerizing the hydroxyl group at the C-2 position of the glucose moiety.
CAS REGISTRY NUMBER
COMMENTARY
37318-37-9
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose
beta-D-mannopyranosyl-(1->4)-D-glucose
-
-
-
?
beta-mannobiose
4-O-beta-D-mannopyranosyl-D-glucose
-
putative product
-
r
globotriose
O-alpha-D-galactopyranosyl-(1,4)-O-beta-D-galactopyranosyl-(1,4)-D-mannose
-
putative product
-
r
additional information
?
-
recombinant enzyme catalyzes a hydroxyl stereoisomerism at the C2-positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides: lactose, beta-mannobiose and globotriose
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-
?
additional information
?
-
-
recombinant enzyme catalyzes a hydroxyl stereoisomerism at the C2-positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides: lactose, beta-mannobiose and globotriose
-
-
?
additional information
?
-
the enzyme catalyzes the epimerization of a D-glucose residue at the reducing end of cellobiose to a D-mannose residue, i.e. cellobiose is converted to beta-D-glucopyranosyl-(1->4)-D-mannose. Enzyme cellobiose 2-epimerase, CE, is highly specific for disaccharides
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-mannobiose
4-O-beta-D-mannopyranosyl-D-glucose
-
putative product
-
r
globotriose
O-alpha-D-galactopyranosyl-(1,4)-O-beta-D-galactopyranosyl-(1,4)-D-mannose
-
putative product
-
r
additional information
?
-
recombinant enzyme catalyzes a hydroxyl stereoisomerism at the C2-positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides: lactose, beta-mannobiose and globotriose
-
-
?
additional information
?
-
-
recombinant enzyme catalyzes a hydroxyl stereoisomerism at the C2-positions of the reducing terminal glucose and at the mannose moiety of cello-oligosaccharides: lactose, beta-mannobiose and globotriose
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5.51
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose
pH 7.5, 30°C, recombinant enzyme
3.7 - 5
cellobiose
recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C
6.56
lactose
recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
104
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose
pH 7.5, 30°C, recombinant enzyme
67.6
cellobiose
recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C
79.5
lactose
recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
18.9
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose
pH 7.5, 30°C, recombinant enzyme
18.1
cellobiose
recombinant enzyme, 100 mM sodium phosphate, 20 mM cellobiose, pH 7.5, 30°C
12.1
lactose
recombinant enzyme, 100 mM sodium phosphate, 20 mM lactose, pH 7.5, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
58.3
recombinant enzyme after first purification step on a DEAE Sepharose FF column
61.8
recombinant enzyme after second purification step on a hydroxyapatite column, substrate is cellobiose
76.78
recombinant enzyme activity in vivo, substrate lactose, pH 7.0, 10°C
79.7
recombinant enzyme after second purification step on a hydroxyapatite column, substrate is lactose
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
45
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function of cellobiose 2-epimerase in carbohydrate metabolism, overview
additional information
enzyme structure-function relationship, overview
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
41100
determined by SDS-PAGE, the mass discrepancy putatively results from partial hydrolysis at the C-terminus during expression or in the purification steps
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by anion exchange chromatography, enzyme is further purified on a hydroxyapatite column
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene bfce, sequence comparisons and phylogenetic analysis, recombinant expression of codon-optimized His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue, the recombinant strain produces lactulose
sequence comparisons and phylogenetic tree, recombinant expression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
application of the enzyme for production of functional oligosaccharides, overview
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Senoura, T.; Taguchi, H.; Ito, S.; Hamada, S.; Matsui, H.; Fukiya, S.; Yokota, A.; Watanabe, J.; Wasaki, J.; Ito, S.
Identification of the cellobiose 2-epimerase gene in the genome of Bacteroides fragilis NCTC 9343
Biosci. Biotechnol. Biochem.
73
400-406
2009
Bacteroides fragilis (Q5LH66), Bacteroides fragilis, Ruminococcus albus (P0DKY4)
Saburi, W.
Functions, structures, and applications of cellobiose 2-epimerase and glycoside hydrolase family 130 mannoside phosphorylases
Biosci. Biotechnol. Biochem.
80
1294-1305
2016
Bacteroides fragilis (Q5LH66), Bacteroides fragilis ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343 (Q5LH66), Caldicellulosiruptor saccharolyticus (A4XGA6), Caldicellulosiruptor saccharolyticus ATCC 43494 / DSM 8903 / Tp8T 6331 (A4XGA6), Cellvibrio vulgaris, Dictyoglomus turgidum (B8DZK4), Dictyoglomus turgidum Z-1310 / DSM 6724 (B8DZK4), Dyadobacter fermentans (C6VW66), Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 (C6VW66), Flavobacterium johnsoniae (A5FA14), Flavobacterium johnsoniae ATCC 17061 / DSM 2064 / UW101 (A5FA14), Herpetosiphon aurantiacus (A9AYF3), Herpetosiphon aurantiacus ATCC 23779 / DSM 785 (A9AYF3), Pedobacter heparinus (C6XVU9), Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 (C6XVU9), Rhodothermus marinus (F8WRK9), Ruminococcus albus (P0DKY4), Saccharophagus degradans (Q21NF7), Saccharophagus degradans 2-40 / ATCC 43961 / DSM 17024 (Q21NF7), Spirochaeta thermophila (E0RU15), Spirochaeta thermophila (G0GBL0), Spirochaeta thermophila ATCC 49972 / DSM 6192 / RI 19.B1 (E0RU15), Spirochaeta thermophila ATCC 700085 / DSM 6578 / Z-1203 (G0GBL0), Spirosoma linguale (D2QDA5), Spirosoma linguale ATCC 33905 / DSM 74 / LMG 10896 (D2QDA5), Teredinibacter turnerae (C5BUF0), Teredinibacter turnerae (K7ZLA5), Teredinibacter turnerae ATCC 39867 / T7901 (C5BUF0), [Eubacterium] cellulosolvens (B3XZI5)
Kuschel, B.; Seitl, I.; Glueck, C.; Mu, W.; Jiang, B.; Stressler, T.; Fischer, L.
Hidden reaction mesophilic cellobiose 2-epimerases produce lactulose
J. Agric. Food Chem.
65
2530-2539
2017
Bacteroides fragilis (Q5LH66), Bacteroides fragilis DSM 2151 (Q5LH66), Butyrivibrio sp. AE2015, Dyadobacter fermentans (C6VW66), Dyadobacter fermentans ATCC 700827 / DSM 18053 / NS114 (C6VW66), Firmicutes bacterium (R7AVK7), Firmicutes bacterium CAG:534 (R7AVK7), Rhodothermus marinus (D0MF61), Rhodothermus marinus (F8WRK9), Rhodothermus marinus ATCC 43812 / DSM 4252 / R-10 (D0MF61), Ruminococcus albus (P0DKY4), Ruminococcus albus DSM 20455 (P0DKY4)
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