EC Number |
Reaction |
Reference |
---|
5.1.3.11 | cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose |
a carbanion-type but no keto intermediate |
2248 |
5.1.3.11 | cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose |
direct reaction at carbon-2 of the reducing moiety of the disaccharide |
2249 |
5.1.3.11 | cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose |
mechanism of reversible epimerization involving three histidine residues, overview. His390 is closely related to ring opening/closure by transferring a proton between the O5 and O1 atoms of the ligand. His259 abstracts the H2 proton of the D-mannose residue at the reducing end, and consistently forms the cis-enediol intermediate by facilitated depolarization of the 2-OH group mediated by hydrogen bonding interaction with His200. His390 subsequently donates the proton to the C2 atom of the intermediate to form a D-glucose residue. The reverse reaction is mediated by the three histidines with the inverse roles of acid/base catalysts |
727985 |
5.1.3.11 | cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose |
reaction mechansim, overview |
-, 747318 |
5.1.3.11 | cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose |
the enzyme's catalytic mechanism involvies three histidine residues, His374 and His243 act as a general acid/base catalyst, and His184 is essential for the enzyme activity, overview |
727529 |