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Information on EC 4.2.3.15 - myrcene synthase and Organism(s) Abies grandis and UniProt Accession O24474

for references in articles please use BRENDA:EC4.2.3.15
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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.15 myrcene synthase
IUBMB Comments
A recombinant enzyme (also known as a monoterpene synthase or cyclase) from the grand fir (Abies grandis) requires Mn2+ and K+ for activity. Mg2+ is essentially ineffective as the divalent metal ion cofactor.
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This record set is specific for:
Abies grandis
UNIPROT: O24474
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Word Map
  • 4.2.3.15
  • terpene
  • synthases
  • monoterpene
  • sesquiterpene
  • herbivore
  • limonene
  • emit
  • lepidopteran
  • enemies
  • gymnosperm
  • herbivore-induced
  • scent
  • jasmonate-induced
  • parasitoids
  • olfactometer
  • cotesia
  • e-beta-ocimene
  • carvone
  • carbocation
  • e-beta-farnesene
  • tepals
  • neryl
  • pinene
The taxonomic range for the selected organisms is: Abies grandis
The enzyme appears in selected viruses and cellular organisms
Synonyms
tps10, myrcene synthase, aatps2, more
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination, C-O bond cleavage
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase (myrcene-forming)
A recombinant enzyme (also known as a monoterpene synthase or cyclase) from the grand fir (Abies grandis) requires Mn2+ and K+ for activity. Mg2+ is essentially ineffective as the divalent metal ion cofactor.
CAS REGISTRY NUMBER
COMMENTARY hide
197462-59-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
myrcene + diphosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
myrcene + diphosphate
show the reaction diagram
induced by stem wounding, enhances resistance to insects
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
required, optimum concentration of 0.5 M
Mg2+
ineffective
Mn2+
required
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
grand fir
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPSD2_ABIGR
627
0
72479
Swiss-Prot
Chloroplast (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
72500
calculated MW of recombinant protein expressed in Escherichia coli
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bohlmann, J.; Steele C.L.; Croteau, R.
Monoterpene synthases from grand fir (Abies grandis): cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase
J. Biol. Chem.
272
21784-21792
1997
Abies grandis (O24474), Abies grandis
Manually annotated by BRENDA team