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Information on EC 4.2.3.120 - (-)-beta-pinene synthase and Organism(s) Artemisia annua and UniProt Accession Q94G53

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.120 (-)-beta-pinene synthase
IUBMB Comments
Cyclase II of Salvia officinalis (sage) produces about equal parts (-)-alpha-pinene, (-)-beta-pinene and (-)-camphene, plus traces of other monoterpenoids. The enzyme, which requires Mg2+ (preferred to Mn2+), can also use (3S)-Linalyl diphosphate (preferred to (3R)-linalyl diphosphate) [1-4]. The enzyme from Abies grandis (grand fir) produces roughly equal parts of (-)-alpha-pinene and (-)-beta-pinene [6-9]. Cyclase IV from Pinus contorta (lodgepole pine) produces 63% (-)-beta-pinene, 26% 3-carene, and traces of alpha-pinene . Synthase III from Pinus taeda (loblolly pine) forms (-)-beta-pinene with traces of alpha-pinene and requires Mn2+ and K+ (Mg2+ is ineffective) . A cloned enzyme from Artemisia annua (sweet wormwood) gave (-)-beta-pinene with traces of (-)-alpha-pinene . The enzyme from Picea sitchensis (Sika spruce) forms 30% (-)-beta-pinene and 70% (-)-alpha-pinene . See also EC 4.2.3.119, (-)-alpha-pinene synthase, EC 4.2.3.117, (-)-camphene synthase, and EC 4.2.3.107 (+)-3-carene synthase.
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Artemisia annua
UNIPROT: Q94G53
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The taxonomic range for the selected organisms is: Artemisia annua
The enzyme appears in selected viruses and cellular organisms
Synonyms
(-)-(1S,5S)-pinene synthase, (-)-pinene synthase, ag9, AvPS, AvTPS1, beta-geraniolene synthase, cyclase II, EC 4.1.99.8, EC 4.2.3.14, mono-tps, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(-)-(1S,5S)-pinene synthase
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beta-geraniolene synthase
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [cyclizing, (-)-beta-pinene-forming]
Cyclase II of Salvia officinalis (sage) produces about equal parts (-)-alpha-pinene, (-)-beta-pinene and (-)-camphene, plus traces of other monoterpenoids. The enzyme, which requires Mg2+ (preferred to Mn2+), can also use (3S)-Linalyl diphosphate (preferred to (3R)-linalyl diphosphate) [1-4]. The enzyme from Abies grandis (grand fir) produces roughly equal parts of (-)-alpha-pinene and (-)-beta-pinene [6-9]. Cyclase IV from Pinus contorta (lodgepole pine) produces 63% (-)-beta-pinene, 26% 3-carene, and traces of alpha-pinene [10]. Synthase III from Pinus taeda (loblolly pine) forms (-)-beta-pinene with traces of alpha-pinene and requires Mn2+ and K+ (Mg2+ is ineffective) [11]. A cloned enzyme from Artemisia annua (sweet wormwood) gave (-)-beta-pinene with traces of (-)-alpha-pinene [5]. The enzyme from Picea sitchensis (Sika spruce) forms 30% (-)-beta-pinene and 70% (-)-alpha-pinene [12]. See also EC 4.2.3.119, (-)-alpha-pinene synthase, EC 4.2.3.117, (-)-camphene synthase, and EC 4.2.3.107 (+)-3-carene synthase.
CAS REGISTRY NUMBER
COMMENTARY hide
110637-20-2
for both EC 4.2.3.119 and 4.2.3.120
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate
(-)-beta-pinene + diphosphate
show the reaction diagram
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products are (-)-alpha-pinene and (-)-beta-pinene, in a ratio of 6:94
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
juvenile leaf
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
QH6_ARTAN
582
0
67507
Swiss-Prot
Chloroplast (Reliability: 4)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcript levels are transiently reduced following mechanical wounding or fungal elicitor treatment
under a photoperiod of 12 h/12 h (light/dark), the abundance of QH6 transcripts fluctuates in a diurnal pattern that ebbs around 3 h before daybreak, i.e. 9th h in the dark phase, and peaks after 9 h in light, i.e. 9th h in the light phase. The contents of (-)--pinene in juvenile leaves and in emitted volatiles also vary in a diurnal rhythm, correlating strongly with mRNA accumulation
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lu, S.; Xu, R.; Jia, J.W.; Pang, J.; Matsuda, S.P.T.; Chen, X-Y.
Cloning and functional characterization of a beta-pinene synthase from Artemisia annua that shows a circadian pattern of expression
Plant Physiol.
130
477-486
2002
Artemisia annua (Q94G53)
Manually annotated by BRENDA team