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Information on EC 4.2.3.114 - gamma-terpinene synthase and Organism(s) Salvia officinalis and UniProt Accession O81193

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.114 gamma-terpinene synthase
IUBMB Comments
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) , Citrus unshiu (satsuma) and Origanum vulgare (oregano) . Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.
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This record set is specific for:
Salvia officinalis
UNIPROT: O81193
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Word Map
The taxonomic range for the selected organisms is: Salvia officinalis
The enzyme appears in selected viruses and cellular organisms
Synonyms
gamma-terpinene synthase, tvtps, ovtps2, ostps24, clcts, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ClcTS
-
-
-
-
OvTPS2
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = gamma-terpinene + diphosphate
show the reaction diagram
major products sabinene and gamma-terpinene are formed by a cyclization mechanism involving a 1,2-hydride shift in the alpha-terpinyl cation intermediate
SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase (cyclizing, gamma-terpinene-forming)
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) [3], Citrus unshiu (satsuma) [4] and Origanum vulgare (oregano) [5]. Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.
CAS REGISTRY NUMBER
COMMENTARY hide
68943-55-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
geranyl diphosphate + H2O
gamma-terpinene + diphosphate
show the reaction diagram
-
63% sabinene, 21% gamma-terpinene, 7% terpinolene, 6.5% limonene, and 2.5% myrcene
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0074
geranyl diphosphate
pH 7.1, 31°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
calculated
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SSS_SALOF
590
0
68942
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68490
x * 68490, calculated
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 68490, calculated
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wise, M.L.; Savage, T.J.; Katahira, E.; Croteau, R.
Monoterpene synthases from common sage (Salvia officinalis). cDNA isolation, characterization, and functional expression of (+)-sabinene synthase, 1,8-cineole synthase, and (+)-bornyl diphosphate synthase
J. Biol. Chem.
273
14891-14899
1998
Salvia officinalis (O81193)
Manually annotated by BRENDA team