Information on EC 4.2.3.106 - (E)-beta-ocimene synthase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
4.2.3.106
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RECOMMENDED NAME
GeneOntology No.
(E)-beta-ocimene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
geranyl diphosphate = (E)-beta-ocimene + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
monoterpene biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
geranyl-diphosphate diphosphate-lyase [(E)-beta-ocimene-forming]
Widely distributed in plants, which release beta-ocimene when attacked by herbivorous insects.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
deduced protein does not contain the RRx8W motif; member of a subfamily of the terpene synthase family designated the Tps-g group, which lacks the RRx8W motif; memeber of TPSg group of terpene synthases
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
; deduced protein contains a modification of the conserved RRx8W motif of plant monoterpene synthases. The N-terminal sequence indicates the presence of a plastid transit peptide
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
enzyme is involved in the herbivore-induced indirect defense response of spider mite-infested Lotus japonicus via de novo formation and emission (E)-beta-ocimene
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
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-
-
?
geranyl diphosphate
(E)-beta-ocimene + diphosphate
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
presence of Mn2+ and Mg2+ required
Mn2+
presence of Mn2+ and Mg2+ required
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
(2E,6E)-farnesyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
0.0067
geranyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0048
(2E,6E)-farnesyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
0.034
geranyl diphosphate
accession Columbia, 30°C, pH not specified in the publication
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
calculated
6.2
calculated
6.3
calculated
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
N-terminal sequence indicates the presence of a plastid transit peptide
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
62000
x * 62000, calculated, x * 63000, SDS-PAGE of recombinant protein
63000
x * 62000, calculated, x * 63000, SDS-PAGE of recombinant protein
63020
x * 63020, calculated
66724
x * 66724, calculated
68800
x * 68800, calculated
69278
x * 69278, calculated
71000
x * 71200, calculated, x * 71000, SDS-PAGE
71200
x * 71200, calculated, x * 71000, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
sequence contains a plastid transit peptide
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Nicotiana tabacum
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
only slight variations in monoterpene synthase mRNA levels during the daily light/dark cycle
transcripts accumulate in leaves in response to exogenous jasmonic acid treatments, lepidopteran herbivory, and lepidopteran oral secretions
transcripts are upregulated in response to mechanical wounding and treatment with jasmonic acid, concurrent with induced emission of (E)-beta-ocimene
transcripts of LjEbOS are induced in plants infested with two-spotted spider mites, Tetranychus urticae, coinciding with increasing emissions of (E)-beta-ocimene as well as other volatiles, (Z)-3-hexenyl acetate and (E)-4,8-dimethyl-1,3,7-nonatriene, by the infested plants. Mechanical wounding of the leaves or application of alamethicin, a potent fungal elicitor of plant volatile emission, also induces transiently increased levels of transcripts, but not elevated release of (E)-beta-ocimene
treatment with the ethylene precursor, 1-aminocyclopropane-1-carboxylic acid does not cause an increase in transcripts
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
when lima bean plants that have previously been placed downwind of transgenic tobacco plants that are constitutively overexpressing (E)-beta-ocimene synthase are infested by spider mites in an open-flow tunnel, they are more defensive to spider mites and more attractive to predatory mites, in comparison to the infested plants that have been placed downwind of wild-type tobacco plants. Similarly, when the transgenic tobacco-downwind maize plants are infested with Mythimna separata larvae, this results in reduced larval growth and greater attraction of parasitic wasps Cotesia kariyai. In a greenhouse experiment, lima bean plants placed near the transgenic tobacco plants are more attractive when damaged by spider mites, in comparison to the infested plants that have been placed near the wild-type plants. Volatile organic compounds emitted from infested receiver plants affect their conspecific neighboring plants to prime indirect defenses in response to herbivory
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LINKS TO OTHER DATABASES (specific for EC-Number 4.2.3.106)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)