geraniol is the main product from geranyl diphosphate in the presence of either Mn2+ or Mg2+, minor products are myrcene, limonene, gamma-terpinene, cis-ocimene, trans-beta-ocimene, terpinolene, linalool, and alpha-terpineol
bicyclogermacrene is the major product in the presence of Mn2+, minor products are (E)-beta-caryophyllene, alpha-humulene, alloaromadendrene, and an unknown sesquiterpene
the content of fruit bicyclogermacrene increases from 85 DAB to 212 DAB. A similar trend is also observed for transcript levels of CmTPS1 during fruit ripening
for leaves, transcripts of CmTPS1 are detected at significantly low levels in young leaves, followed by an increase to mature stage leaves. Significant high concentration of bicyclogermacrene is detected in mature leaves when compared to young leaves
sesquiterpene synthase CmTPS1, EC 4.2.3.150, is associated with bicyclogermacrene formation in Citrus medica fruits, leaves, and flowers, The enzyme level correlates with the concentration of bicyclogermacrene in the tissues
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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
CmTPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, transient recombinant expression in transgenic Citrus seedlings or of GFP-tagged enzyme in Nicotiana benthamiana leaf cytosol via Agrobacterium tumefaciens strain GV3101 transfection method
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
significant increase in bicyclogermacrene is observed in mature leaves after treatment with melatonin for 48 h, accompanying with significant accumulation of transcript levels of CmTPS1
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4.2.3.100 bicyclogermacrene synthase
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