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Literature summary for 4.2.3.100 extracted from

  • Xu, Y.; Wu, B.; Cao, X.; Zhang, B.; Chen, K.
    Citrus CmTPS1 is associated with formation of sesquiterpene bicyclogermacrene (2017), Sci. Hortic., 226, 133-140 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
CmTPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, transient recombinant expression in transgenic Citrus seedlings or of GFP-tagged enzyme in Nicotiana benthamiana leaf cytosol via Agrobacterium tumefaciens strain GV3101 transfection method Citrus medica

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
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Citrus medica 5829
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Citrus medica
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bicyclogermacrene + diphosphate
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?

Organism

Organism UniProt Comment Textmining
Citrus medica A0A1L5JYS1 var. sarcodactylis
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Source Tissue

Source Tissue Comment Organism Textmining
flower during flower development, considerable higher levels are observed for bicyclogermacrene in bloom stage, accompanying higher expression of CmTPS1 Citrus medica
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fruit the content of fruit bicyclogermacrene increases from 85 DAB to 212 DAB. A similar trend is also observed for transcript levels of CmTPS1 during fruit ripening Citrus medica
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leaf for leaves, transcripts of CmTPS1 are detected at significantly low levels in young leaves, followed by an increase to mature stage leaves. Significant high concentration of bicyclogermacrene is detected in mature leaves when compared to young leaves Citrus medica
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
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Citrus medica bicyclogermacrene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
CmTPS1
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Citrus medica
sesquiterpene synthase 1 UniProt Citrus medica

Expression

Organism Comment Expression
Citrus medica significant increase in bicyclogermacrene is observed in mature leaves after treatment with melatonin for 48 h, accompanying with significant accumulation of transcript levels of CmTPS1 up

General Information

General Information Comment Organism
additional information sesquiterpene synthase CmTPS1, EC 4.2.3.150, is associated with bicyclogermacrene formation in Citrus medica fruits, leaves, and flowers, The enzyme level correlates with the concentration of bicyclogermacrene in the tissues Citrus medica