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Information on EC 4.2.2.8 - heparin-sulfate lyase

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.8 heparin-sulfate lyase
IUBMB Comments
Does not act on N,O-desulfated glucosamine or N-acetyl-O-sulfated glucosamine linkages.
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This record set is specific for:
UNIPROT: Q89YS4
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Word Map
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
heparitinase, heparinase iii, heparitinase i, hep i, hepiii, heparin lyase iii, heparan sulfate lyase, heparitin-sulfate lyase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heparan sulfate lyase
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heparin lyase III
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heparin sulfate eliminase
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heparin-sulfate eliminase
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heparitin sulfate lyase
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heparitin-sulfate lyase
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heparitinase I
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heparitinase II
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lyase, heparin sulfate
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
elimination
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elimination of sulfate
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
heparin-sulfate lyase
Does not act on N,O-desulfated glucosamine or N-acetyl-O-sulfated glucosamine linkages.
CAS REGISTRY NUMBER
COMMENTARY hide
37290-86-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
desulfated N-sulfated heparosan
desulfated N-sulfated heparosan disaccharide
show the reaction diagram
-
-
-
?
heparan sulfate
heparan disaccharide
show the reaction diagram
-
-
-
?
heparin
heparin disaccharide
show the reaction diagram
-
-
-
?
heparosan
heparosan disaccharide
show the reaction diagram
-
-
-
?
N-desulfated, N-acetylated heparin
N-desulfated, N-acetylated heparin disaccharide
show the reaction diagram
-
-
-
?
N-sulfoheparosan
N-sulfoheparosan disaccharide
show the reaction diagram
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-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.593
heparan sulfate
pH 7.5, 23°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q89YS4_BACTN
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)
666
0
77547
TrEMBL
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
structures of two crystal forms at resolution 1.8 and 2.4 A. Tyr301 is the main catalytic residue
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H431A
complete loss of activity
N247A
complete loss of activity
Y301F
complete loss of activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ulaganathan, T.; Shi, R.; Yao, D.; Gu, R.X.; Garron, M.L.; Cherney, M.; Tieleman, D.P.; Sterner, E.; Li, G.; Li, L.; Linhardt, R.J.; Cygler, M.
Conformational flexibility of PL12 family heparinases structure and substrate specificity of heparinase III from Bacteroides thetaiotaomicron (BT4657)
Glycobiology
27
176-187
2017
Bacteroides thetaiotaomicron (Q89YS4), Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron DSM 2079 (Q89YS4)
Manually annotated by BRENDA team