EC Number |
Protein Variants |
Reference |
---|
4.2.2.8 | C297S |
site-directed mutagenesis, the mutant suppresses the dimerization and shows 70% reduced activity compared to the wild-type enzyme |
714553 |
4.2.2.8 | C352A |
87% wild-type activity (heparan sulfate), 72% wild-type activity (heparin) |
-, 701885 |
4.2.2.8 | C474A |
32% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin) |
-, 701885 |
4.2.2.8 | C577A |
98% wild-type activity (heparan sulfate), 72% wild-type activity (heparin) |
-, 701885 |
4.2.2.8 | C624A |
24% wild-type activity (heparan sulfate), below 10% wild-type activity (heparin) |
-, 701885 |
4.2.2.8 | E237A |
40.8% of wild-type activitiy |
-, 729269 |
4.2.2.8 | F423A |
42.4% of wild-type activitiy |
-, 729269 |
4.2.2.8 | G112C |
loss of activity |
747570 |
4.2.2.8 | H105A |
PCR overlap extension site-directed mutagenesis, very low expression level, no measurement of activity possible, reduced expression level |
649969 |
4.2.2.8 | H110A |
PCR overlap extension site-directed mutagenesis, reduced kcat, highly reduced Km compared to both recombinant and native wild-type enzymes, reduced expression level |
649969 |