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Information on EC 4.2.1.33 - 3-isopropylmalate dehydratase and Organism(s) Methanocaldococcus jannaschii and UniProt Accession Q58673

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.33 3-isopropylmalate dehydratase
IUBMB Comments
Forms part of the leucine biosynthesis pathway. The enzyme brings about the interconversion of the two isomers of isopropylmalate. It contains an iron-sulfur cluster.
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Methanocaldococcus jannaschii
UNIPROT: Q58673
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Word Map
The taxonomic range for the selected organisms is: Methanocaldococcus jannaschii
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
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Synonyms
isopropylmalate isomerase, leucd, alpha-isopropylmalate isomerase, 3-isopropylmalate dehydratase, ipmi ssu1, isopropylmalate dehydratase, ipm isomerase, mj0499, alpha-ipm isomerase, leu1s, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-isopropylmalate isomerase
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isopropylmalate isomerase
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Alpha-IPM isomerase
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-
-
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alpha-isopropylmalate isomerase
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-
-
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beta-Isopropylmalate dehydratase
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-
-
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dehydratase,beta-isopropylmalate
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-
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IPM dehydratase
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-
-
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IPMI
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-
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isopropylmalate isomerase
MJ0499
gene name, large subunit of 3-isopropylmalate dehydratase
SOI10
-
-
-
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Superoxide-inducible protein 10
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
(2R,3S)-3-isopropylmalate hydro-lyase (2-isopropylmaleate-forming)
Forms part of the leucine biosynthesis pathway. The enzyme brings about the interconversion of the two isomers of isopropylmalate. It contains an iron-sulfur cluster.
CAS REGISTRY NUMBER
COMMENTARY hide
37290-72-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R,3S)-3-isopropylmalate
2-isopropylmaleate + H2O
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2R,3S)-3-isopropylmalate
2-isopropylmaleate + H2O
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron-sulfur cluster
the structure of the protein has unbound Fe-S clusters and contains a fourth cysteine in the active site
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18000
x * 46000 + x * 18000, SDS-PAGE
46000
x * 46000 + x * 18000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 46000 + x * 18000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 25% (w/v) polyethylene glycol monomethyl ether 2000 and 0.1 MTris-HCl (pH 7.9)
sitting-drop vapour-diffusion methodand hanging-drop vapour diffusion at 20°C, structures of oxidized and reduced forms of the large subunit of isopropylmalate isomerase (ox-MJ0499 and red-MJ0499, respectively) are reported at 1.8 and 2.7 A resolution, respectively. Significant large conformational changes are observed in the active site of red-MJ0499 when compared with ox-MJ0499
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
nickel-affinity column chromatography and Superdex 200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli BL21(DE3) Star cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lee, E.H.; Lee, K.; Hwang, K.Y.
Structural characterization and comparison of the large subunits of IPM isomerase and homoaconitase from Methanococcus jannaschii
Acta Crystallogr. Sect. D
70
922-931
2014
Methanocaldococcus jannaschii (P81291), Methanocaldococcus jannaschii, Methanocaldococcus jannaschii DSM 2661 (P81291)
Manually annotated by BRENDA team
Lee, E.H.; Cho, Y.W.; Hwang, K.Y.
Crystal structure of LeuD from Methanococcus jannaschii
Biochem. Biophys. Res. Commun.
419
160-164
2012
Methanocaldococcus jannaschii (Q58673), Methanocaldococcus jannaschii
Manually annotated by BRENDA team