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Information on EC 4.2.1.149 - crotonobetainyl-CoA hydratase and Organism(s) Escherichia coli and UniProt Accession P31551

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.149 crotonobetainyl-CoA hydratase
IUBMB Comments
The enzyme is also able to use crotonyl-CoA as substrate, with low efficiency .
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This record set is specific for:
Escherichia coli
UNIPROT: P31551
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The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Synonyms
crotonobetainyl-coa hydratase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carnithine racemase
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
L-carnitinyl-CoA hydro-lyase [(E)-4-(trimethylammonio)but-2-enoyl-CoA-forming]
The enzyme is also able to use crotonyl-CoA as substrate, with low efficiency [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
crotonobetainyl-CoA + H2O
L-carnitinyl-CoA
show the reaction diagram
-
-
-
r
crotonyl-CoA + H2O
?
show the reaction diagram
-
-
-
?
L-carnitinyl-CoA
(E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O
show the reaction diagram
-
-
-
?
L-carnitinyl-CoA
crotonobetainyl-CoA + H2O
show the reaction diagram
-
-
-
r
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
crotonobetainyl-CoA + H2O
L-carnitinyl-CoA
show the reaction diagram
-
-
-
r
crotonyl-CoA + H2O
?
show the reaction diagram
-
-
-
?
L-carnitinyl-CoA
(E)-4-(trimethylammonio)but-2-enoyl-CoA + H2O
show the reaction diagram
-
-
-
?
L-carnitinyl-CoA
crotonobetainyl-CoA + H2O
show the reaction diagram
-
-
-
r
additional information
?
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
crotonobetainyl-CoA
at pH 7.5 and 37°C
0.038
crotonyl-CoA
at pH 7.5 and 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60.3
cell-free extract, at pH 7.5 and 37°C
699.9
after 11.6fold purification, at pH 7.5 and 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
x * 27000, SDS-PAGE
32329
x * 32329, calculated from amino acid sequence
87000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose column chromatography and Resource Q column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strain K38
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Elssner, T.; Engemann, C.; Baumgart, K.; Kleber, H.P.
Involvement of coenzyme A esters and two new enzymes, an enoyl-CoA hydratase and a CoA-transferase, in the hydration of crotonobetaine to L-carnitine by Escherichia coli
Biochemistry
40
11140-11148
2001
Escherichia coli (P31551), Escherichia coli
Manually annotated by BRENDA team
Eichler, K.; Bourgis, F.; Buchet, A.; Kleber, H.P.; Mandrand-Berthelot, M.A.
Molecular characterization of the cai operon necessary for carnitine metabolism in Escherichia coli
Mol. Microbiol.
13
775-786
1994
Escherichia coli (P31551)
Manually annotated by BRENDA team