show all | hide all No of entries

Information on EC 4.2.1.105 - 2-hydroxyisoflavanone dehydratase and Organism(s) Glycine max and UniProt Accession Q5NUF3

for references in articles please use BRENDA:EC4.2.1.105
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.105 2-hydroxyisoflavanone dehydratase
IUBMB Comments
Catalyses the final step in the formation of the isoflavonoid skeleton. The reaction also occurs spontaneously.
Specify your search results
Select one or more organisms in this record:
This record set is specific for:
Glycine max
UNIPROT: Q5NUF3
Word Map
The taxonomic range for the selected organisms is: Glycine max
The enzyme appears in selected viruses and cellular organisms
Synonyms
2,7,4'-trihydroxyisoflavanone dehydratase, 2-hydroxyisoflavanone dehydratase, GmHID, HID, HIDH, hydroxyisoflavanone dehydratase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-hydroxyisoflavanone dehydratase
278040
-
GmHID
299
-
hydroxyisoflavanone dehydratase
299
-
SYSTEMATIC NAME
IUBMB Comments
2,7,4'-trihydroxyisoflavanone hydro-lyase (daidzein-forming)
Catalyses the final step in the formation of the isoflavonoid skeleton. The reaction also occurs spontaneously.
CAS REGISTRY NUMBER
COMMENTARY hide
166800-10-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,5,7,4'-tetrahydroxyisoflavanone
genistein + H2O
show the reaction diagram
-
-
-
?
2,7,4'-trihydroxyisoflavanone
daidzein + H2O
show the reaction diagram
activity is 40% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone
-
-
?
2,7-dihydroxy-4'-methoxyisoflavanone
formononetin + H2O
show the reaction diagram
activity is 15% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone
-
-
?
p-nitrophenyl butyrate
?
show the reaction diagram
activity is 0.6% of the activity with 2,5,7,4'-tetrahydroxyisoflavanone
-
-
?
additional information
?
-
-
2-hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus
-
-
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
2-hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus
-
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-Br-cGMP
expression of the gene of HIDH is dramatically stimulated by 8-Br-cGMP
NO
stimulation of expression
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
2,5,7,4'-tetrahydroxyisoflavanone
pH 7.5, 30C
0.114
2,7,4'-trihydroxyisoflavanone
pH 7.5, 30C
0.029
2,7-dihydroxy-4'-methoxyisoflavanone
pH 7.5, 30C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
18.1
2,5,7,4'-tetrahydroxyisoflavanone
pH 7.5, 30C
5.3
2,7,4'-trihydroxyisoflavanone
pH 7.5, 30C
1.6
2,7-dihydroxy-4'-methoxyisoflavanone
pH 7.5, 30C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
from seedling
Manually annotated by BRENDA team
-
from seedling
Manually annotated by BRENDA team
-
from seedling
Manually annotated by BRENDA team
-
unifoliate, from seedling
Manually annotated by BRENDA team
-
unifoliate and cotyledonary , from seedling
Manually annotated by BRENDA team
-
exocarp, mesocarp and endocarp
Manually annotated by BRENDA team
-
from seedling
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
from seedling
Manually annotated by BRENDA team
additional information
-
quantitative real-time RT-PCR tissue expression analysis, overview
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
HIDH_SOYBN
319
0
35138
Swiss-Prot
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
introduction of soybean HID into the hairy roots of a model legume Lotus japonicus
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Akashi, T.; Aoki, T.; Ayabe, S.
Molecular and biochemical characterization of 2-hydroxyisoflavanone dehydratase. Involvement of carboxylesterase-like proteins in leguminous isoflavone biosynthesis
Plant Physiol.
137
882-891
2005
Glycine max, Glycine max (Q5NUF3), Glycyrrhiza echinata, Glycyrrhiza echinata (Q5NUF4)
Manually annotated by BRENDA team
Shimamura, M.; Akashi, T.; Sakurai, N.; Suzuki, H.; Saito, K.; Shibata, D.; Ayabe, S.; Aoki, T.
2-Hydroxyisoflavanone dehydratase is a critical determinant of isoflavone productivity in hairy root cultures of Lotus japonicus
Plant Cell Physiol.
48
1652-1657
2007
Glycine max, Lotus japonicus
Manually annotated by BRENDA team
Suita, K.; Kiryu, T.; Sawada, M.; Mitsui, M.; Nakagawa, M.; Kanamaru, K.; Yamagata, H.
Cyclic GMP acts as a common regulator for the transcriptional activation of the flavonoid biosynthetic pathway in soybean
Planta
229
403-413
2009
Glycine max, Glycine max (Q5NUF3)
Manually annotated by BRENDA team
Livingstone, J.M.; Zolotarov, Y.; Stroemvik, M.V.
Transcripts of soybean isoflavone 7-O-glucosyltransferase and hydroxyisoflavanone dehydratase gene homologues are at least as abundant as transcripts of their well known counterparts
Plant Physiol. Biochem.
49
1071-1075
2011
Glycine max, Glycine max Williams 82
Manually annotated by BRENDA team
Select items on the left to see more content.