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Information on EC 4.2.1.104 - cyanase and Organism(s) Synechocystis sp. and UniProt Accession Q55367

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EC Tree
     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.104 cyanase
IUBMB Comments
This enzyme, which is found in bacteria and plants, is used to decompose cyanate, which can be used as the sole source of nitrogen [6,7]. Reaction (1a) can be considered an equivalent of 'cyanate + H2O = carbamate', where the water molecule is provided by the dehydration of bicarbonate to carbon dioxide , and hence the enzyme is classified as a hydrolase.
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This record set is specific for:
Synechocystis sp.
UNIPROT: Q55367
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The taxonomic range for the selected organisms is: Synechocystis sp.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
hide
cyanate
+
HCO3-
+
2
H+
=
NH3
+
2
CO2
+
+
2
=
+
2
cyanate
+
HCO3-
+
H+
=
carbamate
+
CO2
+
+
=
+
cyanate
+
HCO3-
+
2
H+
=
NH3
+
2
CO2
+
+
2
=
+
2
Synonyms
cyanase, cyanate hydratase, atcyn, cyanate lyase, oscyn, slr0899, spcyns, cyanate aminohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyanase
cyanate aminohydrolase
-
-
-
-
cyanate C-N-lyase
-
-
-
-
cyanate hydratase
-
-
-
-
cyanate hydrolase
-
-
-
-
cyanate lyase
-
-
-
-
EC 4.3.99.1
-
formerly
Hydrolase, cyanate
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cyanate + HCO3- + 2 H+ = NH3 + 2 CO2
show the reaction diagram
the enzyme requires bicarbonate as a cofactor. Its mechanism is to catalyse the attack of bicarbonate on cyanate, with elimination of carbon dioxide, thus catalysing hydration of the cyanate to carbamate. The carbamate spontaneously hydrolyses to ammonia and carbon dioxide
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carbon-nitrogen lyase reaction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
carbamate hydro-lyase
This enzyme, which is found in bacteria and plants, is used to decompose cyanate, which can be used as the sole source of nitrogen [6,7]. Reaction (1a) can be considered an equivalent of 'cyanate + H2O = carbamate', where the water molecule is provided by the dehydration of bicarbonate to carbon dioxide [2], and hence the enzyme is classified as a hydrolase.
CAS REGISTRY NUMBER
COMMENTARY hide
37289-24-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
NCO- + HCO3- + H+
NH3 + CO2
show the reaction diagram
NCO- + HCO3-
NH4+ + CO2
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NCO- + HCO3- + H+
NH3 + CO2
show the reaction diagram
utilization of exogenous cyanate as a niche source of C and N in cyanobacteria
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NH4+
-
negative regulation, represses enzyme expression
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bicarbonate
-
-
NtcA
-
transcription factor, global nitrogen regulator of cyanobacteria, required for expression
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
cyanase activity shown in whole-cell extracts of Synechocystis, high level of cyanase activity when grown with nitrate as N source but unable to utilize exogenous cyanate to support O2 evolution in the light
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain PCC6803
SwissProt
Manually annotated by BRENDA team
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, cyanase gene cynS not arranged in a three-gene cluster
gene cynS, tightly clustered with 4 putative molybdenum cofactor biosynthesis genes located downstream
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molecular biology
analyses of conditions to metabolize exogenously supplied cyanate
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Harano, Y.; Suzuki, I.; Maeda, S.; Kaneko, T.; Tabata, S.; Omata, T.
Identification and nitrogen regulation of the cyanase gene from the cyanobacteria Synechocystis sp. strain PCC 6803 and Synechococcus sp. strain PCC 7942
J. Bacteriol.
179
5744-5750
1997
Synechococcus sp., Synechocystis sp.
Manually annotated by BRENDA team
Espie, G.S.; Jalali, F.; Tong, T.; Zacal, N.J.; So, A.K.
Involvement of the cynABDS operon and the CO2-concentrating mechanism in the light-dependent transport and metabolism of cyanate by cyanobacteria
J. Bacteriol.
189
1013-1024
2007
Synechococcus elongatus, Synechococcus elongatus (Q59948), Synechococcus elongatus PCC 7942, Synechococcus elongatus PCC 7942 (Q59948), Synechococcus sp., Synechococcus sp. (Q5N0J1), Synechococcus sp. UTEX625, Synechocystis sp. (Q55367)
Manually annotated by BRENDA team